Application Notes: LC-MS

Learn how proper water purification eliminates PFAS background, enabling ultra sensitive LC MS/MS analysis with confidence using EPA 1633 ready ultrapure water outputs.

A practical guide to LC–MS calibration, comparing external, surrogate and in sample approaches to control matrix effects and support accurate, regulator aligned bioanalysis.

This application note highlights a new multi-modal LC-MS/MS approach enabling robust analysis of polar and ionic metabolites without derivatization or ion-pairing.

Discover how Arium® Comfort I UV system supports precise PFAS detection, reducing contaminants to sub-ppt levels while improving sustainability and cost efficiency.

Separations of Oligonucleotides using HALO 1000 Å OLIGO C18 show improved peak capacity, narrower peaks, and lower backpressure vs. 300 Å FPP columns.

HALO 1000 Å OLIGO C18 enables high-resolution impurity analysis of 90-mer ssDNA, outperforming competitors in peak capacity and separation efficiency.

Larger pore columns improve efficiency and reduce band dispersion in oligonucleotide separations-especially at high flow rates and longer chain lengths.

Longer columns paired with scaled gradients enhance oligonucleotide resolution especially for complex mixtures up to 100 nucleotides in length.

Discover how HALO® OLIGO C18 columns improve DNA/RNA separation with optimized ion pairing, pH control, and particle morphology for faster, sharper results.

Improve validated HPLC methods by switching from fully porous to CORTECS™ Premier C18 5 µm Columns to boost throughput, cut solvent use, and enhance method greenness.

This application note describes the separation of the 20 standard PFAS according to Directive (EU) 2020/2184 in only eight minutes.

n this application note, a USP monograph is modernized to a shorter column using 5 µm particles, which can be allowable under USP <621> as long as the N value is within the guidelines. By using highly efficient CORTECS 5 μm particles, this type of modernization is possible and reductions in solvent usage and run times are achieved

In this work the development of two key attributes of the 5um CORTECS Columns is examined. First column efficiency is compared across CORTECS and other solid-core 5 μm columns. Next scalability from sub-2 μm to 5 μm particles is examined between the CORTECS Column lines and competitive column lines. It was found that CORTECS columns have higher efficiency compared to other solid-core columns and that CORTECS particles are fully scalable.

Ultrashort-chain PFAS (C1–C4) are important due to their widespread presence and here is an application solution for this analysis.

Slalom chromatography separates nucleic acids >3 kbp in <6 minutes using shear-based molecular stretching for high-resolution, gel-free analysis.

he United States Pharmacopeia (USP) has designations for all columns stationary phases used in the monograph methods. These designations outline the stationary phase type, i.e. fully porous or solid-core, and any ligand attachments, i.e. C18 or Phenyl to be used.1 However, beyond that no column specifics are given. With a multitude of columns that fit into the different designations, understanding that not all columns are the same is vital when selecting a stationary phase for a monograph method. This application note examines three columns that all fit into the L1 designation when analyzing paracetamol impurities. Selectivity differences between the columns are considered in relation to the impurities.

Explore how wide-pore SEC delivers sharp, reproducible separation of megadalton-sized DNA vectors and plasmids with minimal secondary interactions.

We evaluated the accuracy of lipid quantitation in human plasma by LC-MS using single-point or multipoint calibration curves with authentic or surrogate standards.

This SEC-MS method demonstrates the precise separation of a single-chain variable fragment from its drug-conjugate.

In this application note the charge variant characterisation of IgG4-based mAbs was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.

This application note provides you an optimum RP method for the DAR determination of a site-specific ADC using a widepore YMC-Triart Bio C4 column.

This application with data from Roche Diagnostics confirms superior performance of bioinert-coated YMC Accura BioPro IEX SF columns for the IEX-MS analysis of mAbs.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.

With this AEX method a mix of six oligonucleotides can be separated in under two minutes.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.

This application with data from Roche Diagnostics confirms superior performance of bioinert-coated YMC Accura BioPro IEX SF columns for the IEX-MS analysis of mAbs.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.