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Click the title above to open The Column November 23, 2015 North American issue, Volume 11, Number 21, in an interactive PDF format.

LCGC Blogger Tony Taylor offers some simple visual methods to assess column performance.

They say a picture paints a thousand words… This month I’ve taken inspiration from a recent webcast, presented at www.chromacademy.com, in which I presented real data from our work that represents some ‘classic’ GC problems.

In this video, Robert T. Kennedy of the University of Michigan discusses the role of liquid chromatography–mass spectrometry (LC–MS) in the rapid development of metabolomics and the challenge of dealing with all the data. He then goes onto talk about the methods used in a recent study on glucose metabolism to analyse complex metabolic pathways and the challenges of using smaller samples.

Column overload is a very commonly encountered issue in GC for beginners. Changes in peak symmetry, generally observed as peak fronting, can be subtle in the sharp peaks generated by GC, but the result can be significant shifts in retention times, loss of resolution, and error in peak integration.

The characterization of paint in famous artworks is valuable for investigating the painting techniques used by artists, for conservation purposes and to help repair damaged paintings. By combining HPLC–ESI-QTOF-MS with traditional analytical techniques, researchers were able to clarify the composition of the oil components and hypothesize the botanical origin of the lipid materials used by Edvard Munch.

Kevin Schug discusses why we need a comprehensive study of unconventional oil and natural gas (UOG) extraction processes.

Click the title above to open The Column November 06, 2015 Europe & Asia issue, Volume 11, Number 20, in an interactive PDF format.

Click the title above to open The Column November 06, 2015 North American issue, Volume 11, Number 20, in an interactive PDF format.

The second part of the review on the two-day symposium focusing on the characterization of biopharmaceutical molecules at AstraZeneca’s MedImmune site in Cambridge, UK.

A new method for the simultaneous determination of emerging and persistent organic pollutants (POPs) in fish bile samples has been developed using gas chromatography–mass spectrometry (GC–MS).

Accounting for the inherent heterogeneity of monoclonal antibodies is essential to ensure production of consistent and safe therapeutics and, in light of this, there is an array of chromatographic and supporting regulatory systems. Robust characterization and analysis of monoclonal antibodies is becoming a fast and straightforward process thanks to the myriad technological advances underpinning this rapidly evolving area.

Incognito offers his rules for a chromatographer’s life.

Ron Majors’s personal recollections on the first 50 years of column development-with perspectives on what was going on behind the scenes, particularly during the exciting early days-including a summary of his early publications that contributed to development of HPLC column technology.

Ron Majors’s personal recollections on the first 50 years of column development-with perspectives on what was going on behind the scenes, particularly during the exciting early days-including a summary of his early publications that contributed to development of HPLC column technology.

The term dead volume often comes up in chromatography discussions and literature. This month we address the nature of this phenomenon, when it can become a problem that affects chromatographic results, and how to understand and take control of it.

Primary record is a term that was defined by the MHRA (Medicines and Healthcare products Regulatory Agency, the UK drug regulator) in data integrity guidance issued in 2015. In this instalment of Questions of Quality we explore what this term means in practice, and compare it with raw data in the European Union Good Manufacturing Practices (EU GMPs) and complete data in US Food and Drug Administration (FDA) GMPs. Why can’t we have harmonization of terms?

A summary of the results from a survey of 14 leading HPLC–UHPLC column experts is presented, covering the state of sub-2-?m porous particles, superficially porous (core–shell) particles, silica monoliths, and polymeric monoliths.

This article presents a method for comparing the levels of baseline interference arising from common laboratory mobile phase contamination sources and assesses different approaches for removing dissolved contaminants to generate interference-free chromatogram baselines. The authors demonstrate that recirculating mobile phase through a semi-preparative scale column using a reagent delivery pump has advantages over previously published mobile phase decontamination methods.

In gas chromatography (GC) split injection systems, as much as 99% or more of the injected sample never enters the column: It is released downstream of the inlet where it can encounter and possibly perturb precision gas control devices. The split-vent trap acts to prevent or at least moderate such effects. This month’s instalment addresses the operation and maintenance of split-vent traps.

Are your solvent costs too high? Here are some ideas on how to decrease the amount of mobile phase you use.

Throughout his career, Ron Majors always had his finger on the pulse of the latest chromatography developments, and he kept readers in the know too.

An unbiased summary of the state of the art up to mid-1994, which actually marked the first 27 years of HPLC.

Hamish Small spoke to AndrО Striegel about the secrets of his success in separation science, including the development of ion chromatography, and the value of vague thoughts in scientific progress.

An excerpt from LCGC’s e-learning tutorial on UV detection for HPLC at CHROMacademy.com

Paul R. Loconto shares his insights into the principles and practice of implementing and improving upon the original method to quantitate cyanide at trace concentration levels in human blood matrices using automated cryogenic trapping isotope dilution static headspace gas chromatography-mass spectrometry (GC-MS).