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Following on our previous Dedicated Dialogue which discussed lab water contaminants and purification technologies, this Dedicated Dialogue will consider specific ways to handle and manage a lab water purification system and the product water from these systems in order to ensure consistent and reliable results.

Characterization of macromolecules and colloids is an area of considerable interest. Asymmetrical flow field-flow fractionation (AF4) has become a well-established method, but many potential users possess limited knowledge of its capabilities, or how it can provide additional information or serve as validation to the traditional analytical techniques. This review article highlights several practical applications where AF4 should be given special consideration, and discusses benefits and drawbacks of the different methods.

The market for lactose-free products is growing rapidly and constantly and Europe is a worldwide leader in the lactose‑free market. Between 2012 and 2016 the sales of lactose-free products are expected to increase by 75%.

How to get started in the process of identifying the problem source for column-related problems.

Following on our previous Dedicated Dialogue which discussed lab water contaminants and purification technologies, this Dedicated Dialogue will consider specific ways to handle and manage a lab water purification system and the product water from these systems in order to ensure consistent and reliable results.

Researchers at Zhejiang University, China, have developed a new method combining liquid chromatography-mass spectrometry (LC-MS) and off-line nuclear magnetic resonance (NMR) to analyze the constituents within traditional Chinese medicines (TCMs).

A new screening procedure has been developed for the detection of common “non banned” drugs used by athletes. The method targets multiple pharmacologically active compounds by liquid chromatography-electrospray ionization coupled to tandem mass spectrometry (LC-ESI-MS-MS).

Click the title above to open The Column November 23, 2015 Europe & Asia issue, Volume 11, Number 21, in an interactive PDF format.

Click the title above to open The Column November 23, 2015 North American issue, Volume 11, Number 21, in an interactive PDF format.

They say a picture paints a thousand words… This month I’ve taken inspiration from a recent webcast, presented at www.chromacademy.com, in which I presented real data from our work that represents some ‘classic’ GC problems.

In this video, Robert T. Kennedy of the University of Michigan discusses the role of liquid chromatography–mass spectrometry (LC–MS) in the rapid development of metabolomics and the challenge of dealing with all the data. He then goes onto talk about the methods used in a recent study on glucose metabolism to analyse complex metabolic pathways and the challenges of using smaller samples.

Column overload is a very commonly encountered issue in GC for beginners. Changes in peak symmetry, generally observed as peak fronting, can be subtle in the sharp peaks generated by GC, but the result can be significant shifts in retention times, loss of resolution, and error in peak integration.

The characterization of paint in famous artworks is valuable for investigating the painting techniques used by artists, for conservation purposes and to help repair damaged paintings. By combining HPLC–ESI-QTOF-MS with traditional analytical techniques, researchers were able to clarify the composition of the oil components and hypothesize the botanical origin of the lipid materials used by Edvard Munch.

Kevin Schug discusses why we need a comprehensive study of unconventional oil and natural gas (UOG) extraction processes.

“A Toolbox for LC–MS Protein Analysis” from EMD Millipore will be held on 17 and 18 November 2015.

Click the title above to open The Column November 06, 2015 Europe & Asia issue, Volume 11, Number 20, in an interactive PDF format.

Click the title above to open The Column November 06, 2015 North American issue, Volume 11, Number 20, in an interactive PDF format.

The second part of the review on the two-day symposium focusing on the characterization of biopharmaceutical molecules at AstraZeneca’s MedImmune site in Cambridge, UK.

A new method for the simultaneous determination of emerging and persistent organic pollutants (POPs) in fish bile samples has been developed using gas chromatography–mass spectrometry (GC–MS).

Accounting for the inherent heterogeneity of monoclonal antibodies is essential to ensure production of consistent and safe therapeutics and, in light of this, there is an array of chromatographic and supporting regulatory systems. Robust characterization and analysis of monoclonal antibodies is becoming a fast and straightforward process thanks to the myriad technological advances underpinning this rapidly evolving area.

Incognito offers his rules for a chromatographer’s life.

Ron Majors’s personal recollections on the first 50 years of column development-with perspectives on what was going on behind the scenes, particularly during the exciting early days-including a summary of his early publications that contributed to development of HPLC column technology.

Ron Majors’s personal recollections on the first 50 years of column development-with perspectives on what was going on behind the scenes, particularly during the exciting early days-including a summary of his early publications that contributed to development of HPLC column technology.

The term dead volume often comes up in chromatography discussions and literature. This month we address the nature of this phenomenon, when it can become a problem that affects chromatographic results, and how to understand and take control of it.

Primary record is a term that was defined by the MHRA (Medicines and Healthcare products Regulatory Agency, the UK drug regulator) in data integrity guidance issued in 2015. In this instalment of Questions of Quality we explore what this term means in practice, and compare it with raw data in the European Union Good Manufacturing Practices (EU GMPs) and complete data in US Food and Drug Administration (FDA) GMPs. Why can’t we have harmonization of terms?

A summary of the results from a survey of 14 leading HPLC–UHPLC column experts is presented, covering the state of sub-2-?m porous particles, superficially porous (core–shell) particles, silica monoliths, and polymeric monoliths.