Liquid Chromatography (LC/HPLC)

A wide range of instruments, columns and media are available for LC purification, including Flash and HPLC. Are these techniques interchangeable? How do they compare?

Mark Woodruff of Fortis Technologies talks about the background, advantages and applications of hydrophilic interaction chromatography and where its future lies.

The authors discuss an LC method for the direct analysis of diquat and paraquat in lake water.

A change in the mobile phase strength can be a powerful tool to move peaks in a separation.

Whether organic produce provides any actual health benefits is still controversial, and a recent study of organic carrots, onions and potatoes has claimed that the process may add nothing at all.

KNAUER has donated a high-quality analyser to the Hamburg University of Technology (TUHH) for use in education and training in the chemical engineering department.

Agilent Technologies has announced the opening of a new life sciences instrument manufacturing facility in Singapore. The facility will produce the company's LC-MS instruments for the global market.

The advantages of HPLC-Chip-MS workflow over conventional methods of N-glycan analysis

Direct-Electron Ionization is a useful tool in LC–MS. This article discusses the potential of this technique as a universal detector for small molecule analysis and how to deal with matrix effects.

If something doesn't look right you need to investigate!

A simple and rapid HPLC method was established to simultaneously determine the active ingredients of red clover.

John Dolan presents some practical pointers to get the most out of your UHPLC system.

This article highlights a number of recent developments in HPLC for polyphenol analysis.

The guest authors describe an optimization procedure to achieve the highest plate count in a given analysis time.

Accurate and sensitive all-ions quantification using ultra-high resolution LC-MS

Ultra high performance liquid chromatography (UHPLC) systems, also referred to as fast LCs, are designed to take full advantage of smaller particles (sub 2 ?m), which yield higher resolution and increased sensitivity.

Speaking to Konik-Tech about the development of a new system combining LC and GC technology.

Using SRM and H-SRM on an Integrated UHPLC-MS Platform for Sensitive, Accurate and Reproducible Quantification of Perfluorinated Compounds in Human Breast Milk.

Practical HPLC, HILIC and RP-LC methods of analysing common food and beverages containing polyphenols are presented.

John Dolan discusses an inquiry he received from a reader complaining about the difficulty he was having meeting system suitability for an LC method that had been transferred into his lab.

The principles and attributes of today's HPLC detectors are discussed, and the advantages and disadvantages of the various detectors are compared and contrasted.

Incognito provides a retrospective account on subjects visited throughout the course of the year and asks if any problems have been addressed and what new problems have arisen.

A study published in the Journal of Separation Science has looked at using the racemization of amino acids as a proxy for DNA preservation, giving archaeologists a rapid screening method for samples too badly damaged to extract.

A simple rapid HPLC method for simultaneously determiing formononetin, biochanin A, daidzen and genistein in Red Clover

Guest author Stefan Brand and John Dolan take a look at how to speed up gradient separations while avoiding common errors.