Liquid Chromatography (LC/HPLC)

The use of UHPLC has triggered a remarkable increase in efficiency and throughput of LC and LC –MS methods. The fastest growing market segment in the pharmaceutical industry is biopharmaceuticals, with an increased interest in applying UHPLC to biopharmaceutical analysis.

Achieving a high quality sample separation is critical to the success of UHPLC analyses. Numerous practical factors must be considered to achieve this.

A novel method for analysing mercury in sediment is described.

From the invention of eluent suppression to today's "just add water" concept, pivotal developments over the last 40 years are chronologically highlighted from a chemical and instrumental viewpoint.

The first instalment of the Perspectives in Modern HPLC column highlights some of the new HPLC systems, accessories and related technology introduced at Pittcon this year.

Multiple fractions can be tested in parallel with this fast method.

There are many factors that influence the performance of a high performance liquid chromatography (HPLC) stationary phase, of which the chemical nature of the bonded phase ligand is important, but by no means all encompassing. Minor manufacturing parameters such as the method of electropolishing the internal surface of the column can also have an effect on the selectivity and efficiency produced by a particular column.

Incognito found a 2D LC solution to a tricky separation problem but this technique did not meet the client's requirements. The magnanimous columnist admits to a rare defeat on this occasion but praises the thrill of seeking a solution to a perplexing method development problem. Could any readers suggest a more straightforward solution?

In honor of LCGC's celebration of 30 years covering the latest developments in separation science, we asked a panel of experts (listed in the sidebar) to assess the current state of the art of liquid chromatography (LC) column technology, and to try to predict how the technology will develop in the future. This article is part of a special group of articles covering the state of the art in sample preparation, gas chromatography (GC) columns, GC instrumentation, LC columns, and LC instrumentation.

Automated column and mobile phase screening is the current trend in the pharmaceutical industry to develop chiral methods. This study presents comprehensive strategies for automated rapid chiral HPLC method development including normal-phase, reversed-phase and polar organic phase separation modes.

Some of the newer hydrophilic interaction liquid chromatography (HILIC) columns are reviewed, and experiments performed to categorize columns for optimized separations are described.

Monolithic columns feature both high column efficiency and high column permeability. This article discusses their preparation, applications, and future prospects.

A simple and sensitive high performance liquid chromatography (HPLC) method with ultraviolet (UV) detection was developed to analyse phenylurea herbicides: – monuron, diuron, linuron, metazachlor and metoxuron – in three soft drink brands and tap water.

A mysterious autosampler problem is solved.

A systematic way to develop HPLC methods consists of building up and running a useful set of experiments (DOE, design of experiments) to evaluate the influence of several factors or method conditions on the selectivity of a chromatogram. The crucial part here is the peak tracking between the chromatograms.

The results of a recent survey on high performance liquid chromatography (HPLC) columns shows some interesting changes.

The practical aspects of hollow-fiber liquid-phase microextraction in the three-phase mode (HF3LPME) are described.

A review of HPLC 2011, which took place in Budapest, Hungary in June this year.

The GPC/SEC column is the key component for the separation of macromolecules by size. It enables a conventional HPLC instrument to be used for GPC/SEC analysis.

Flash chromatography is a type of preparative liquid chromatography commonly used in the separation of organic compounds.

The 2011 Manfred Donike award for scientific excellence in sports-doping testing has been presented to Simon Beuck and Nicolas Leuenberger.

Waters has welcomed the Proteomics Core Facility at the Duke University School of Medicine, Durham, North Carolina, USA, into its Centers of Innovation programme.

In this review, practical principles and guidelines for designing LC?LC methods are given.

Incognito draws similarities between advances in car technology and HPLC systems. Better end products have come at the cost of knowledge about how it actually works and can be maintained.

The Singapore Eye Research Institute will advance biomarker candidates that it has identified for chronic inflammatory eye conditions into large-scale clinical trials for validation using AB SCIEX technology.