Liquid Chromatography (LC/HPLC)

The analysis of clozapine and norclozapine in plasma using automated on-line sample preparation and LC–MS/MS

Deterioration of the separation can result from many causes. This month's column looks at some techniques to isolate the problem source.

UHPLC is fast becoming mainstream in many laboratories. With increased data, however, comes a directly proportional increase in data integration.

Weikang Bio-Technology Group, a Chinese manufacturer and marketer of traditional Chinese medicines, is launching a new project to research and develop liquorice flavonoids for use in therapeutics. For the study, the company reports that it intends to use supercritical carbon dioxide extraction technology to produce superior quality herbal extracts, column chromatography to isolate and purify compounds and HPLC to separate, identify and quantify compounds based on their idiosyncratic polarities.

Global health and safety concerns now play a key role in driving innovation of liquid chromatography systems and the uptake of advanced LC technology, such as UHPLC.

John Dolan looks at a case in which the peak doesn't seem to belong with the neighboring peaks in the chromatogram.

The basics of IC are reviewed and recent advances that may extend this technology to new applications are discussed.

SFC is now regarded as a modern normal phase LC technique prompting a re-examination of the role of bare silica as a useful stationary phase.

A broad peak where it doesn't belong is a classic sign of late elution.

Atmospheric pressure gas chromatography is a technique that allows rapid switching between UPLC-MS and GC-MS applications.

We focus on the characterization of reversed-phase columns with particular emphasis on the hydrophobic-subtraction model

A relatively small set of columns can be used on a routine basis in different modes to meet modern analytical needs.

We focus on the characterization of reversed-phase columns with particular emphasis on the hydrophobic-subtraction model

This is John Dolan's 300th "LC Troubleshooting" column. Here, he takes a look through the archives.

John Dolan reaches a milestone with his 300th installment of "LC Troubleshooting" and takes a look back through the years.

This article aims to demonstrate a novel accelerated method development approach to develop HPLC and/or UPLC methods

This month Incognito reflects on his own laboratory's strategies for column care and shares their working practices for increasing the longevity of each column and preventing degradation.

In recent conversations at Pittcon 2010, it became clear to the author that sometimes we get too focused on the details of the method without backing up and determining how the fit into the big picture.

Michael Swartz presents his first annual review of HPLC systems and accessories at Pittcon 2010.

Part one focusses on ways of reducing and replacing solvents commonly used as mobile phases

Pat Sandra and his team will discuss how green chromatography works in practice

The separation of polar and hydrophilic compounds can be a challenge. Hydrophilic interaction liquid chromatography has proven to be a solid alternative for such applications.

As I travelled the miles of poster boards and sat through the various lectures, it became apparent that many of us still don?t know how to choose or optimize buffers for liquid phase separations.

Incognito comes up with a collection of rules of thumb for the relationships and mathematics behind web-based translators and calculators used for deriving results.

Oxytocin acetate samples were analysed successfully for both the peptide and acetate with a single method using a mixed-mode weak-anion-exchange column.