Liquid Chromatography (LC/HPLC)

Accelerating pharmaceutical studies by using LC-MS ion trap technology to conduct comprehensive metabolism profiling assays

The need for better methods for vitamin D analysis in a wide variety of foods is a good showcase for the advantages that ultrahigh-pressure liquid chromatography (UHPLC) can provide. The availability of systems that can operate as high as 1200 bar and an extensive range of UHPLC column choices provide the flexibility needed to rapidly develop fast vitamin D separations that can be adapted to meet the challenges of testing a diversity of food types

This article will demonstrate the feasibility of analysing complex mixtures without any prior sample preparation by making use of the high resolving power of both UHPLC and mass spectrometry.

The latest innovations in separation science will be revealed at Pittcon 2010 from 28 February–5 March 2010 in Florida, USA

John Dolan examines some questions he recently received concerning the changes that took place in the 2009 edition of the United States Pharmacopoeia.

Participants in this discussion are Deanna Hurum of Dionex, Michael Dong of Genentech, and William Foley of Waters Corp.

This month's installment of "Innovations in HPLC" discusses the use of HPLC for these recent threats to health and safety.

What should you do when a peak is too small or too large?

A rapid and environmentally friendly LC method for the simultaneous determination of 12 UV filters in cosmetic samples using ethanol as the mobile phase.

John Dolan looks at ways to get to the root of some of the most common problems encountered in the lab.

In the inaugural installment of his new column, LCGC veteran Michael Swartz provides an overview of some of the newer LC technology used in contemporary LC laboratories.

This article describes methods to quantitatively analyse genotoxic and potentially genotoxic impurities in pharmaceutical ingredients

A fast clean and simple method to determine three organotin compounds in environmental samples is described

Kevin Schug talks to Harold McNair about his illustrious career in chromatography

Guest author Uwe Neue develops the principle of kinetic plots from scratch to make them more accessible to a wider audience.

The authors present the first installment in their examination of the basics of method validation.

In this installment of "Column Watch," Ron Majors looks at the results of a recent HPLC column survey conducted by LCGC North America.

Dionex Corporation (Sunnyvale, California) will be hosting a series of free seminars on liquid chromatography throughout the United States and Canada from September 9?October 29, 2009.

In Part II, we review MS ionization suppression; column, pH, and temperature selection; and system peaks and column equilibration issues associated with the use of five volatile perfluorocarboxylic acid reagents in LC.

What's the best way to integrate a peak?

Advion BioServices (Ithaca, New York) announced the expansion of its existing LC–MS Biomarker Services Group, which was launched in 2008 to develop and deliver customized assays for peptide, amino acid, lipid, neurotransmitter, and nucleotide biomarkers.

A novel way of separation for polyolefins.

How to determine PFCs in water by SPE-LC–MS.

John Dolan looks at the signal-to-noise ratio and its relationship to uncertainty in a measurment.

An optimization strategy to obtain the best possible performance in the shortest analysis time for comprehensive off-line 2D-LC.