Liquid Chromatography (LC/HPLC)

This month, Chromatography Online's Technology Forum looks at the topic of HPLC/TLC and the trends and issues surrounding it. Joining us for this discussion are Eike Reich from Camag, Gerda Morlock, from University of Hohenheim, and Colin poole, from Wayne University.

Polymer-based columns still are widely used in size-exclusion chromatography (SEC) and ion chromatography-ion-exchange chromatography - they have always been used in these modes, even back in 1987.

The unique features of silica hydride-based columns are described with a focus on how these columns can expand a laboratory's capabilites in HPLC and LC-MS. Of particular interest is the ability to retain both polar and nonpolar compounds and provide an orthogonal method of analysis for currently existing procedures.

Shimadzu Scientific Instruments (Columbia, Maryland) and Groton Biosystems (Boxborough, Massachusetts) recently announced a collaboration effort to interface Groton’s online sampling monitors with Shimadzu’s high performance liquid chromatography (HPLC) system to form a closed-loop solution for online analysis.

Gilson, Inc. (Middleton, Wisconsin), an industry leader in purification systems for high performance liquid chromatography (HPLC), solid phase extraction (SPE), and gel permetation chromatography (GPC) clean-up, now offers direct support and sales in the United States.

Thermo Fisher Scientific Inc., a leader in the separation sciences and chromatography industries, announces the launch of its enhanced partner program. The program is designed to support the expansion of the companies' informatics activity via its partners around the globe, the world class program will utilize business partners to better meet customer needs in multiple industries. Thermo Scientific?s informatics offerings include laboratory information management systems, chromatography data systems, and spectroscopy software.

This article describes the factors that affect the selection of columns for two-dimensional (2D) LCÃ-LC separations. The maximum increase in peak capacity compared with single-dimension (1D) separations is obtained by using "orthogonal" systems employing various combinations of separation mechanisms to provide as different separation selectivities as possible for the sample compounds in the first and in the second dimension systems. To obtain best results, matching the chemistry of the stationary phase, column dimensions and mobile phases in the first and in the second dimensions is essential for successful separations, especiall for comprehensive LCÃ-LC.

Getting started on the right foot is important for efficient method development.

How to avoid an expensive shot in the dark.

An ultrahigh-pressure liquid chromatography (UHPLC) method was developed to separate paroxetine from several of its related compounds using a systematic screening protocol that monitors combinations of selectivity factors including column chemistry, organic modifier, and pH. When the best combination of these factors was selected, the method was optimized by varying gradient slope and temperature.

HPLC 2007 was held in Ghent, Belgium in June. Last month, columnist Ron Majors summarized some the important column developments as well as other Symposium highlights. This month, he winds up coverage with additional highlights in the areas of technology and applications. Among the topics covered are stationary phase preparation and characterization, multi-dimensional and comprehensive LC, temperature studies, detectors and an application overview.

This report shows the steps involved in the development of a new polymethyl methacrylate (PMMA) calibration standard for polar organic GPC applications.

The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, an event that features the newest applications and technologies in the chromatography industry, have announced the 2008 Pittsburgh Conference Memorial National College Grants (PCMNCG) Program.

Eksigent, a developer of advanced low-flow fluid delivery liquid chromatography technologies, announced distribution agreements with three companies in Europe for sales and service of the company's high performance liquid chromatography (HPLC) and liquid chromatography (LC) product lines.

Leipziger Messe, organisers of L.A.B. - the trade fair and conference devoted to analysis, bio and laboratory equipment - have announced that the show will host 88 exhibitors from countries including the UK, US, France, Italy, Finland, the Netherlands, and Germany. L.A.B. takes place from 2-4 October 2007 at ExCeL London.

Ronald E. Majors, LCGC North America columnist and Senior Chemist for the Columns and Supplies Division of Agilent Technologies, Inc., has been awarded the 2007 Martin Gold Medal by the Chromatographic Society in the United Kingdom for outstanding contributions to the chromatography field.

Waters Corporation (Milford, Massachusetts) and Lambda Therapeutic Research Ltd (Mumbai, India) recently announced a joint cooperative agreement that provides innovative analytical laboratory equipment designed to enhance research quality service and business solutions for Lambda and its clients.

Science Daily, a website that features news revolving around the sciences, has published an article that describes new chromatographic techniques for the analysis of pesticides and antibiotics in food and beverage samples.

Applied Biosystems (Foster City, California) recently announced three new value-added services for laboratories that are utilizing Applied Biosystems/MDS SCIEX mass spectrometry (MS) instrument systems as part of an overall workflow solution.

Nanostream, provider of products and services that increase productivity in laboratories at pharmaceutical and biotech companies, extends its global reach to Europe by establishing a sales and support office in The Netherlands.

Biodiesel Magazine has recently posted an article that gives an overview of biodiesel test methods, which includes gas chromatography (GC), infrared spectrometry (IR), and inductively coupled plasma (ICP).

The Victoria Advocate (Victoria, TX) published an article about the random testing of high school student-athletes in the Texas public school system. The article is entitled "No Steroid Test is Foolproof" and focuses on the process of drug testing using gas chromatography-mass spectroscopy (GC-MS).

The Montreal Gazette published an article about the use of analytical techniques in the wine industry. The author discusses the difference between "natural" wines and "industrial" wines that are created with a certain taste profile in mind and with the assistance of gas chromatography.

AC Analytical Controls developed a new chromatographic solution that complies with all ASTM & EN chromatographic test methods listed in biodiesel specifications: the AC all-in-one Biodiesel analyser. Analysis results demonstrate that the AC analyser complies with EN 14103, EN 14105, EN 14106, EN 14110 & ASTM D6584 methods.

Different methods require different strategies.

Cleaning validation is a major analytical application in the pharmaceutical industry. Here, high performance liquid chromatography (HPLC) with charged aerosol detection is compared and contrasted to HPLC with UV detection showing comparable performance and several advantages for charged aerosol detection, especially for analytes that do not contain a chromophore.

In the fourth installment in a series on method development for liquid chromatography (LC), with an emphasis on devloping trouble-free methods quickly. John Dolan started out by considering some of the goals we might have and some method development strategies.

Columnist Ron Majors covers some of the highlights of HPLC 2007 including honorary sessions, best poster awards, and the latest developments in HPLC column technology.

Liquid chromatography (LC) separations performed at elevated temperatures offer the following advantages.