Peer-Reviewed Articles

A method based on GC–MS coupled with magnetic SPE with multi-walled carbon-nanotube-coated Fe3O4 as adsorbent was developed for the analysis of four organophosphate esters (OPEs) in ambient water samples. The method was validated for its comparable sensitivity and potential application prospect.

The UHPLC–MS/MS method can accurately determine the presence of these illegal feed additives in swine tissues.

Improved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.

Py–GC–MS has advantages for the analysis of environmental microplastic samples compared to other leading analytical methods, including spectroscopic techniques.

HS-SPME-GC–MS was combined with OPLS-DA data analysis to tentatively identify eight chemical markers to differentiate the geographical origins of cigar leaf samples.

GC and TLC methods are demonstrated for quantification of stigmasterol 3-O-β-D-glucopyranoside (S3G), the main active component in the herbal nutraceutical Balanites aegyptiaca, an antihyperglycemic in Egyptian folk medicine.

This article discusses the development of a series of applications that will allow for the determination of a number of nitrosamines that have been identified by the FDA as genotoxins to monitor, initially showing how to perform a separation of these compounds from the API and then looking at how MS can be applied to the analysis to ensure that the required detection limits can be reached.

Ensuring complete overlapping (coelution) of analyte and internal standard peaks can be critical for eliminating matrix effects in LC–MS/MS analysis.

This exploration into commercially available light scattering detection systems for size-exclusion chromatography (SEC) emphasizes new technologies and will help users understand the options.

Decomposing animal tissue releases volatile organic compounds (VOCs), of interest in forensic science. We describe the use of GC×GC–qMS/FID retrofitted with a reverse fill/flush (RFF) flow modulator for analyzing these VOCs in a tropical climate.

We assess recent advances in cleaning verification methods for small-molecule pharmaceuticals, and propose a workflow.

A UHPLC–MS/MS method is described for rapid quantification of five major bioactive alkaloids in rat urine. The results obtained help lay the foundation for the clinical application and safety evaluation of the bioactive ingredients of menispermi rhizoma, used in herbal medicines.

Recent advances in stationary phases for hydrophobic interaction chromatography (HIC) permit HIC–MS analysis of intact antibodies and other proteins using direct flow to the mass spectrometer.

A method is described using a triple quadrupole LC–MS instrument with isotopic dilution to obtain the highest accuracy and confidence for analysis of per- and polyfluoroalkyl substances (PFAS) in water. Excellent method spike recoveries and robustness were found in wastewater.

We investigate a phenomenon observed by many practitioners of reversed- phase LC: a dramatic loss in retention volume or time after the column flow is stopped and resumed when using highly aqueous mobile phases with certain columns.

In many applications, LC–MS approaches using HILIC, SFC, SEC, IEC, and HIC offer advantages over conventional reversed-phase LC–MS. The examples presented here should inspire you to consider these options for your analyses.

In this study, a method was developed for extraction and preconcentration of trace amounts of organophosphorus pesticides (OPPs), including fenitrothion, chlorpyrifos-methyl, and chlorpyrifos in environmental water.

Low-pressure gas chromatography (LPGC)–MS should be the first option in many GC–MS applications to provide fast, sensitive, and robust analyses; and the installation, instrumentation, and implementation of LPGC is possible in any GC–MS system.

Quantification of European Union (EU)-priority polycyclic aromatic hydrocarbons (PAHs) in plant matrices is a crucial task. Various methods for enrichment and preconcentration, such as the preloaded-pipette tip solid‑phase extraction (SPE) (1), are available. Nevertheless, analyte recovery as a result of homogenization, sample preparation, and extraction are rarely discussed in the field of phytopharmacy. This study deals with the recovery in dry plant extracts, which are typically used in phytopharmaceuticals and reflect the actual polycyclic aromatic hydrocarbon content in the commercially available end product (2). The aim of this study was to monitor benzo[a]pyrene, benzo[a]anthracene, chrysene, and benzo[b]fluoranthene loss of spiked samples as a result of commonly-used sample pretreatment, extraction, filtering, and evaporating techniques in 1:1 (v/v) cyclohexane–ethyl acetate primulae flos and sambuci flos dry extracts. Results showed that improper sample preparation can lead to false results. In the case of benzo[a]pyrene with a deviation of 155% from the theoretical true value.

A simple LC–MS method has been developed and validated for the simultaneous determination of 18 synthetic food additives and caffeine in soft and energy drinks, and in various alcoholic beverages. Nine food colours (tartrazine, sunset yellow FCF, azorubine, ponceau 4R, allura red AC, patent blue V, brilliant blue FCF, green S, brilliant black BN), two preservatives (sorbic and benzoic acid) and seven sweeteners (acesulfame K, aspartame, cyclamic acid, saccharin, sucralose, neohesperidin DC, neotame) were targeted food additives. The method employs reversed-phase ultra-high performance liquid chromatography (UHPLC) for analyte separation and a single quadrupole mass spectrometer for their detection. The limits of quantification were low enough to enable a reliable control of maximum limits set for some additives (Regulation [EC] No. 1333/2008). The method was applied for analysis of a wide range of samples collected at a typical supermarket: 14 soft drinks, 19 energy drinks, and 43 alcoholic beverages.

Gas chromatography–mass spectrometry (GC–MS), reversed-phase LC with stop-flow fluorescence (FL), and constant energy synchronous fluorescence spectroscopy (CESFS) are explored to determine PAH isomers in three combustion-related standard reference materials.

Clinical metabolomics requires analytical methods that provide both high resolution and high throughput. In this review, we assess the promise of a variety of techniques-including HILIC, SFC, multidimensional LC, ion-mobility mass spectrometry, and data-independent acquisition mass spectrometry methods-to meet those needs.

This integrated analytical approach combines HPLC–MS/MS and GC–MS/MS to detect 36 PFAS migrating into food simulants from packaging.

With these equations, you can predict how solute dilution will affect sensitivity.

The versatile size-exclusion ultrahigh-performance liquid chromatography (SE-UHPLC) platform method described here provides superior separation for bispecific monoclonal antibody formats compared to a previous method.

The sum of ranking differences (SRD) is a useful statistical tool for comparing methods, models, columns, or samples. It is also simple and straightforward.

Biomimetic HPLC retention data can be used to measure how a compound will bind to proteins and phospholipids in vivo.

We explore the developments that have made it possible to produce sub-minute, and, more recently, even sub-second enantiomeric separations. We also look at the questions that remain unanswered.

The series continues with a crucial scientific and regulatory necessity in ensuring reliable information-the second-person review.

The theoretical plate concept forms the basis of chromatographic theory. Here is where it came from.