Application Notes: General

PSG® Biotech offers a comprehensive portfolio of pumps, sensors and flow meters engineered to protect media integrity and product quality while producing the highest yields – from laboratory to commercial scale.

The latest release of Thermo Scientific™ Chromeleon™ CDS redefines the standards for software performance with solid system stability, industry-leading ease of use, and speedy operation.

Once APIs move into later phases of development, an advanced impurity control strategy is vital and demands a comprehensive analytical methods approach.

There is an ever increasing demand for bespoke laboratory space for science and technology companies like yours due to the individual nature of your research and operations. Bruntwood SciTech makes acquiring custom laboratory space easy, reliable and affordable, so your business can form, scale and grow.

A breakthrough for adsorbable and extractable organic fluorine (AOF and EOF) and how different SPE phases are used for accurate determination of PFAS.

Adsorbable organically bound fluorine (AOF) is used to screen water for PFASs. DIN 38409-59 outlines AOF analysis with pyrohydrolytic combustion and ion chromatography.

HDX-MS with online EC reduction enables successful epitope mapping of mAbs to challenging S-S bonded protein targets, e.g., VEGF. The biophysical characterization of VHH-IR4, a BBB-crossing single-domain antibody (sdAb) is described for the first time and was only possible using EC reduction.

High-Performance Anion Exchange Chromatography in combination with PAD or MS detection is the method of choice for the analysis of all classes of carbohydrates including glycans. A novel anion-exchange stationary phase SweetSep™ has been developed that allows for fast and high resolution separation.

Shodex collaborated with Claros Technologies using HILICpakTM VT-50 2D Shodex column to study PFAS in industrial and ground water samples. Per and polyfluoroalkyl substances (PFAS) have been in industrial use since the 1960’s and are commonly found in the environment.

Different classes of antibodies, including IgG1, 2 and 4, are immediately reduced down to light and heavy chain without the need of enzymes, denaturing or reducing agents using inline EC-LCMS. The workflow can be used for IdeS digested mAbs and does not affect the quantitation of acid labile species such as sialylated N-glycans.

Process and Quantitate All xC/UV/MS Data in a Single Interface with Software Compatible with Major Vendor Formats.

The AEX200 column demonstrate (a) fast separation of mono-, di- and trisaccharides in various food sample matrices, (b)sensitive quantification of carbohydrates down to femtomole levels, (c) high-resolution separation of high-molecular weight oligosaccharides with a degree of polymerization up DP of 60.

Separation of all 6 glycan monosaccharides of interest and 2-Deoxy-D-Glucose (glycosylation inhibitor) was achieved within 7 minutes using the new SweetSep™ AEX20 column under isocratic elution conditions. Furthermore, profiling of released N-glycans from glycoproteins without the need for sample derivatization is shown using HPAEC-MS.

Read how the purchase of a new UPLC instrument motivated Takeda to simplify analytical data processing for their Process and Medicinal chemists by deploying Spectrus Processor.

This application note discusses the use of the LC Simulator module to accurately model retention and peak width of proteins and peptides in analytical scale reversed phase and ion exchange chromatography.

Read about how AutoChrom supported high quality, consistent separations, and helped finalize a method in 2 days that the Dow AgroSciences team had spent months trying to optimize.

Using the structure verification and knowledge management capabilities in MS Structure ID Suite, Medtronic has created a data-handling workflow for chemical characterization and database curation.

The retention time prediction capabilities of ACD/Chromgenius are outlined, and a suggestion of how these can be incorporated into a typical investigative water monitoring workflow is also included.

Retention modeling has proven to be a successful technique in accelerating method development and optimization. This case study gives a more detailed insight into how scientists at Merck use computer-assisted modeling to develop robust methods faster.

The new Revident LC/Q-TOF system is leveraged for the screening of pesticides in complex matrix utilizing data independent acquisition (DIA). Confident identification is made through a combination of high-resolution, extended dynamic range, stable accurate mass, and isotopic fidelity which can be quickly and easily analyzed via the customizable LC Screener Tool, simplifying the processing of complex screening data. Along with this screening capability, new automated software, Intelligent Reflex, uses the results and allows for the reinjection and targeted confirmation of questionable and/or identified residues. This reduces the amount of time from sample introduction to confident characterization.

Explores the ways in which tech bio is transforming the life science industry. Hear from Dr Kath Mackay, Director of Life Sciences at Bruntwood SciTech and leaders from NorthWest EHealth, Elixir Software and Cellular Highways as they share their perspectives on how tech bio has advanced their businesses.

Complementarity in chromatography ensures accurate purity. Daicel offers non-chiral stationary phases compatible in different modes for cyclic peptide analysis.