Application Notes: General

This poster demonstrates the utilization of a highly sensitive and accurate LC-MS/MS method for the quantitation of select endogenous steroids using dried blood spots (DBS) serum separator cards.

In this study, the automatized Agilent 1290 Infinity II system was used for purification of native single stranded oligonucleotides (ONs). The separations were performed with IP-RPLC using dibutylamine (DBA) and tris(hydroxymethyl) aminomethane (TRIS). The combined separation methods with instrumental setup with columns, allowed for the target compound to be collected faster and with higher purity, compared to conventional methods.

The work illustrates a single LCMS method for the analysis of AZBT and AMBBT in five sartan drug substances, with full separation of all sartans and azido impurities achieved in only 8.5 minutes.

LabSolutions DB/CS software offers powerful support for ensuring compliance with data integrity requirements for all types of analytical instruments, and can be optimized for a wide range of laboratory sizes and operating styles. Download this file to learn more.

In this poster, the 40 PFAS from EPA method 1633 were used to generate a library from a single mixture, and then were confirmed to function for targeted library screening and untargeted identification in groundwater.

This work demonstrates the benefits of adding a compact single quad LCMS into an existing workflow for API screening and analysis.

Demonstrated in this work is the use of bioinert UHPLC and LC-MS to analyze oligonucleotides accurately and reproducibly, allowing for faster development and quality control.

In this poster, a triple quad LC-MS was used to quantify per- and poly-fluoroalkylated substances (PFAS) to meet the requirements set by the EPA 1633 draft method, which describes the analysis of forty PFAS in wastewater, solids, biosolids, and tissue samples.

This poster evaluates the performance of the end-to-end workflow for the extraction, screening, identification, and quantitation of over 80 sphingolipids from 10 classes in plasma, a method using Agilent 1290 Infinity II LC coupled to an Agilent 6495 triple quadrupole LC/MS system.

This poster exhibits how combining XF and LC/MS technologies can provide deeper insight into the cellular and molecular metabolic response to drug treatment for cancer research.

This poster illustrates the outstanding quantification performance for large panel pesticide screening analysis in a known difficult matrix such as cinnamon, using a novel LC/TQ platform, including the Agilent 1290 Infinity II Bio LC system coupled to the 6495 Triple Quadrupole LC/MS with the Jet Stream Technology Ion Source (AJS).

This study assesses a workflow developed for PFAS analysis in wastewater, based on the existing EPA draft Method 1633 with additional PFAS of varying size and functional group. This workflow contains off-line solid phase extraction (SPE) and a clean-up step, followed by LC-MS/MS analysis, and automatic reporting using SLIMS. The workflow demonstrates a reliable solution for the targeted analysis of PFAS in complex matrices with high robustness.

This study demonstrates the 6495 LC/TQ (G6495D) is suitable for comprehensive protein quantification assays in complex matrix.

This poster demonstrates how the HILIC polar metabolite workflow is enhanced with the new 4th generation 6495 LC/TQ, and robust quantitation is enabled with new 13C metabolite transitions.

This poster shows that over 200 pesticides screened for using a DIA mode of acquisition from 0.625 ppb to 100 ppb​,​ good Mass Accuracy, Low RSD’s, and Scored Coelution of Fragment Ions​ are demonstrated.

This poster demonstrates that the Agilent 6495 triple quadrupole LC/MS system shows increased analytical sensitivity of nitrosamine impurities at the low concentration levels specified by regulatory requirements.

PSG® Biotech offers a comprehensive portfolio of pumps, sensors and flow meters engineered to protect media integrity and product quality while producing the highest yields

In an ever-evolving cannabis industry where regulatory compliance and accurate testing are paramount, staying ahead requires a deep understanding of how to build an efficient testing lab that meets compliance standards:

Calibrating detectors and monitoring devices with accurate and traceable gas mixtures in air monitoring applications is important for human and environmental health.

This article explores how to use this powerful, free, simulation tool to simplify and speed up LC–MS/MS method development.

This application note provides insight into how solvents can be efficiently dried down using a nitrogen blowdown evaporator and a guide on the best place to start.

Accurate purity assessment of oligonucleotide mixtures can be challenging due to their complexity. Daicel offers several solutions that provide unique selectivity.

Complementarity in chromatography ensures accurate purity. Daicel offers non-chiral stationary phases compatible in different modes for cyclic peptide analysis.

This short video provides an overview of a complete, end-to-end oligonucleotide workflow solutions

Characterization of oligonucleotides requires robust analytical instrumentation and methods as well as ease-of-use data analysis tools. Biocompatibility mitigates non-specific sample binding to flow path and it ensures the integrity of biomolecules and robustness of the system. In this study, two workflows, the Target Plus Impurities (TPI) and Sequence Confirmation workflows in Agilent MassHunter BioConfirm software, were carried out to characterize two oligonucleotide samples.