Application Notes: General

Accurate purity assessment of oligonucleotide mixtures can be challenging due to their complexity. Daicel offers several solutions that provide unique selectivity.

Complementarity in chromatography ensures accurate purity. Daicel offers non-chiral stationary phases compatible in different modes for cyclic peptide analysis.

This short video provides an overview of a complete, end-to-end oligonucleotide workflow solutions

Characterization of oligonucleotides requires robust analytical instrumentation and methods as well as ease-of-use data analysis tools. Biocompatibility mitigates non-specific sample binding to flow path and it ensures the integrity of biomolecules and robustness of the system. In this study, two workflows, the Target Plus Impurities (TPI) and Sequence Confirmation workflows in Agilent MassHunter BioConfirm software, were carried out to characterize two oligonucleotide samples.

In this application note, the determination of oligo sequence confirmation using HILIC LC and high-resolution MS/MS data is described. As with the previous studies, an InfinityLab Poroshell 120 HILIC-Z column was used along with an Agilent 6545XT AdvanceBio LC/Q-TOF mass spectrometer.

Xylazine has emerged as a prevalent adulterant in illicit drugs. A robust SPE and LC-MS/MS method for analyzing xylazine, fentanyl, and other adulterants is presented.

In this application note, LC separation and MS1 mass identification of a variety of oligos without the use of ion‑pairing reagents is demonstrated. The LC separation allows subsequent positive mode use with little to no flushing or hardware changes. This HILIC-based method uses an Agilent InfintyLab Poroshell 120 HILIC-Z column and MS-friendly ammonium acetate-based mobile phases. The samples were analyzed on an Agilent 1290 Infinity II LC system and a 6545XT AdvanceBio quadrupole time-of-flight mass spectrometer (LC/Q-TOF).

End-to-End Workflow Solutions for Oligonucleotide Analysis - From research discovery to production QA/QC

UV-Vis spectrophotometers have been used widely for nucleic acid quantification and quality control (QC) utilizing the fact that nucleic acids have a maximum absorbance at 260 nm (1). The concentration of nucleic acids can be easily estimated using the absorbance at 260 nm and the established absorption coefficient. Often a background correction is also performed, for example collecting a baseline using a solution containing everything but the nucleic acid or by measuring the absorbance at a wavelength that nucleic acids do not absorb. Double stranded nucleic acids are bound by hydrogen bonds between the base pairs. The temperature at which double stranded nucleic acids denature to become single stranded depends on the: – sequence and length of the nucleic acid – the pH and buffer conditions – and any mismatches in base pairs between the two strands As such, the melting temperature is very useful analytical tool and can be studied by monitoring the absorbance at 260 nm as temperature is increased or decreased. As the temperature is increased, the hydrogen bonds between the strands are broken and the double stranded nucleic acid separates into two separate strands. When the strands separate, the absorbance at 260 nm increases. The transition temperature is called “melting temperature” (Tm) (1).

Despite the utility of the essential oil mixture, it has been shown that allergies can arise from some of the individual components.

Automated, high-sensitivity analysis of residual fumigants for food safety testing by multi-step enrichment–headspace–trap (MSE–HS–trap) with GC–MS.

Determine down to 8x10^9 AAV capsids and separate impurities of AAV samples by separation using a mixed-bed SEC column & highly sensitive MALS detection.

The CDS 8500 Purge and Trap electronic pressure control (EPC) is used for automated preparation of calibration curves.

The CDS 8500 Purge and Trap system is used to perform automated sample dilution to prepare calibration curves.

This application note demonstrates a novel Empore 8270 Disk to extract 125 semi-volatile organic compounds from water samples per EPA 8270E method.

This application note introduces a sample-stacking technique to improve sensitivity for low concentration polymer samples.

A rapid, automated polymer degradation study of polymers containing UV stabilizers under ultraviolet (UV) light in a temperature-controlled environment.

Empore StageTips was widely used in peptides and proteins desalting in Proteomics. The sample loading amount on total number of protein identifications was evaluated.

This application note presents data on a rapid, automated polymer degradation study of rubber under ultraviolet (UV) light in air.

SweetSep is a new line of HPAEC columns from Antec Scientific for superior separation of all classes of carbohydrates including N-glycans using PAD or MS detection.

Reliable and reproduceable liquid handling results depend crucially on the regular test and calibration of the instruments. The globally most accepted International Standardization Organization (ISO) standard that details requirements for producing and in-use control of piston-operated volumetric apparatus (POVA) is the ISO 8655. The aim of this white paper is to present the requirements for gravimetric calibration and testing of piston-operated volumetric apparatus (POVA) according to part 6 and part 7 of the ISO 8655 revised in 2022. This white paper presents the differences between calibration and testing according to chapters 6 and 7 , describes the testing method as well as testing environment, testing equipment and reporting requirements.

Bulb and graduated pipettes made of glass or plastic are common in the laboratory, especially when pipetting volumes greater than 1,000 µL. One example of graduated pipettes are serological pipettes. Plastic (disposable) serological pipettes are widely used in cell culture applications. There are multiple reasons to replace serological pipettes with Sartorius pipettes. Discover the three main reasons to switch to a Sartorius pipette.

ISO 8655:2022 contains updated requirements for producing and in-use control of piston-operated volumetric apparatus (POVA), replacing the 1st edition from 2002. ISO 8655:2022 consists of nine parts in total. Here, we describe the main points about Part 2: Pipette

A disadvantage of modern LIBs is instability of the electrolytes. Learn about the use of IC for analysis of decomposition products to evaluate electrolyte degradation.

Pipettes and pipette accessories are staples in most laboratory environments, from biochemistry to pharmaceuticals to beverage quality control. Because different assays have different needs in terms of equipment and technique, it is important to assess the particular set of needs required in your lab. Key requirements for most modern-day applications include: Accuracy, minimizing human errors, speed, easy-to-clean-instruments, precision, ability to decontaminate and avoiding contamination.

In this guide, Sartorius explores some factors to consider when choosing a manual or electronic pipette for microbiological analyses. Requirements for liquid handling in a microbiology lab might be different from other analytical laboratories. Here are some points to consider before committing to purchasing a pipette

Thermo Scientific ProPac 3R SAX columns provide excellent separation of Protein G charge variants using a salt gradient. Its unique design delivers high resolution, robust performance, and excellent reproducibility needed for charge variant analysis of proteins.

Designed with bio-inert materials, the new Thermo Scientific ProPac 3R columns support the delivery of reproducible, high resolution and robust strong anion and strong cation exchange chromatography separations for protein and monoclonal antibody analysis.