Application Notes: General

Identification and quantification of the Raffinose family of oligosaccharides allow a more thorough understanding of an individual’s gut health.

The measurement of adenosine nucleotides represents important diagnostic measures concerning metabolic standing for the determination of various pathological states.

Modified nucleosides, either natural or synthetic, often provide RNA with distinctive properties useful in diagnostics or therapeutics.

Ion pairing agent aided separation of oligonucleotides from their potential failure sequences, highlighting the Hamilton family of reversed-phase HPLC columns.

Whether the application is wet, dry, or cryogenic, consistent and viable representation of sample preparation leads to more accurate and repeatable results for analysis.

This note summarises a procedure developed by the US Food and Drug Administration to measure 16 PFAS in food using a QuEChERS sample preparation approach and LC–MS/MS analysis.  

Purification of peptide-peptoid hybrids can be obtained by reversed phase chromatography with excellent recovery and resolution.

The ability to collect compounds is directly related to the ability to detect them. Many compounds possess weak or non-existent chromophores which causes UV detection to exhibit poor yields.

The development of comprehensive liquid chromatography–high resolution accurate mass workflows for confident identification and mapping of oligonucleotides.

PFAS are known to be persistent in the environment. Detecting low levels of PFAS in drinking water is made easier using an Activated Carbon Delay Column.

Sampling and analysis of a challenging range of trace-level volatile & semi-volatile vapours of per- & polyfluorinated alkyl substances (PFAS) in air using TD–GC–MS

The effect of various ion pairing reagents (amines and acidic counterions) on the LC–UV and LC–MS analysis of oligonucleotides (ONs) has been studied.

Use software to deconvolute LC–MS spectra from complex samples and identify unknown components.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions. 

The Agilent MassHunter Optimizer software for GC Triple Quad (GC/TQ) can help generate multiple reaction monitoring transitions for newly added compounds.