Application Notes: General

Automated MHC-I is associated peptide enrichment using the Agilent AssayMAP Bravo platform with large-capacity Protein A cartridges for immunopeptidomics analysis.

The AssayMAP Bravo was used to purify, digest, and clean up antibodies from 96 cell culture supernatants with high reproducibility and minimal labor prior to LC–MS.

Intact and subunit antibody mass determination was streamlined using automated purification and enzymatic cleavage and deglycosylation of immobilized antibodies.

Automated sample prep and LC–MS peptide mapping approach to characterize and quantify multiple monoclonal antibody (mAb) critical quality attributes (CQAs)

Automated phosphopeptide enrichment from cell lysates with Fe(III)-NTA enables the identification of thousands phosphopepides with high reproducibly and selectivity.

Automated phosphopeptide enrichment and ion mobility mass spectrometry (IMS-MS) enables detailed analysis of structural effects of site-specific phosphorylation.

Automated Antibody Drug Conjugate (ADC) purification and deglycosylation increases reproducibility and simplifies drug-to-antibody ratio (DAR) determination

Field-flow fractionation with online static and dynamic light scattering constitutes a powerful and versatile system for nano-pharmaceutical characterization.

This application note presents a reversed-phase LC method capable of separating all relevant reduction-induced LMW species of the NISTmAb.

Get a better understanding of how combustion ion chromatography is an effective and sensitive analytical technique for AOF.

Easily implement a robust, reliable, and reproducible workflow solution for the analysis and quantitation of nine HAAs, bromate, and dalapon in water using IC–MS/MS.

This application note will illustrate how a new integrated pump controller is giving users automated control of pump operations in critical biopharma applications.

This application note reveals the Vanquish Neo offers maximal MS utilization for direct injection workflows and versatility to separate peptides at low flow rates.

This application note shows a micro-flow LC–MS method developed on the Vanquish Neo UHPLC is suitable for high-throughput proteomic analyses of large sample cohorts.

The hydrolysis and SPE steps were combined for designer and pharmaceutical benzodiazepines from urine along with a fast quantitative LC–MS/MS method.

A low-level extraction of steroids using Novum PRO SLE is performed and compared with another SLE product for the baseline cleanliness.

ZipChip CE-MS offers a fast and easy method for native charge variant analysis and product monitoring workflows that are often challenging with traditional LC-MS.