Application Notes: General

Shandong Loncom Pharmaceutical Co. implemented NuGenesis LMS to help with generation of reliable and compliant production data, address electronic data management challenges, achieve accurate and efficient sample tracking, and manage increasing amounts of data. Learn more about how NuGenesis™ LMS enables labs to collect, record, and analyze experimental data for improved operational efficiency and data accuracy.

This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.

Now there's an single-purpose HPLC with a simplified UI that makes it possible for anyone to perform HPLC testing with a sinple one-button pass/fail test function.

Welcome to our comprehensive selection of High-Performance Liquid Chromatography (HPLC) cartridges. Designed for precision and reliability, our cartridges cater to diverse analytical needs, ensuring optimal performance in your laboratory processes.

The solution is an automated reaction monitoring system capable of unattended sampling, identification, and quantification of reaction components via HPLC. Axcend system is the only separations Process Analytical Technology (PAT) system that’s small enough to be placed in a hood or immediately adjacent to the reaction vessel.

Application Notebook highlighting Liquid Chromatography techniques commonly used for monitoring Critical Quality Attributes (CQAs) in Bioprocessing Applications

This application note demonstrates how THF mobile phases can be replaced to implement greener, safer, and less toxic SEC methods for polymer characterization.

Agilent and Scitara are collaborating not only to increase the efficiency of laboratory processes, but to enable customers to leverage the value of the data for various stakeholders. This paper will present an ecosystem designed to support enterprises in accelerating data workflows and improving data availability for researchers, lab analysts, and data scientists.

This study demonstrates high-throughput analysis of BTEX compounds from several matrices (air, water and soil). Detection limits in the single-digit part-per-billion concentration range (by volume) are readily achievable within seconds using SIFT-MS because sample analysis is achieved without chromatography, pre-concentration, or drying. We also present a calibration approach that enables speciation of ethylbenzene from the xylenes in real-time.

This application note summarizes key SIFT-MS results presented in a peer reviewed article entitled “Unreported VOC Emissions from Road Transport Including from Electric Vehicles.” Learn how the Wolfson Atmospheric Chemistry Laboratory at the University of York used SIFT-MS for VOC analysis in its platform to experimentally verify that motor vehicle screen wash is a significant unreported source of VOC emissions (especially for ethanol and methanol).

Method detection limits (MDLs) have been determined for the newly regulated HON (Hazardous Organic NESHAP (National Emission Standards for Hazardous Air Pollutants)) compounds, which validate selected ion flow tube mass spectrometry (SIFT-MS) as an effective solution for measuring these toxic volatile organic compounds (VOCs) and other environmental pollutants in ambient air, whether at the fenceline or in a mobile setting. SIFT-MS offers unparalleled speed, sensitivity and ease of use for environmental monitoring applications.

A comparison made between a stainless steel hardware column and an INERT column using the same lot of HALO 90 Å C18, 2.7µm results in tailing factor and area improvement using the HALO®INERT column.

Using HALO®Elevate C18 and a high pH mobile phase obtains a great separation of drugs of abuse and metabolites without the use of a specialized stationary phase.

A 16 mer ssDNA poly dT oligonucleotide was analyzed under UV conditions on a HALO®OLIGO C18 column. The results revealed smaller peaks before and after the primary peak of interest.

The positively charged surface chemistry of HALO® PCS Peptide delivers narrower peak width, higher signal response and double the peak capacities compared to standard uncharged peptide materials

Over the course of 5000 injections, the HALO®Elevate C18 1.5 mm ID column demonstrates stability and robustness in the stationary phase and the column hardware, with no significant changes to the retention time, peak shape, and back pressure.

A mix of four antidepressants is separated using the HALO®PCS (positive charged surface) C18 column demonstating the improved tailing factor and efficiency when compared to a traditional (uncharged) C18 stationary phase.

The HALO® OLIGO C18 2.7 µm column outperformed an FPP Oligo C18 column in oligonucleotide separations, achieving faster separations while maintaining efficiencies comparable to a 1.9 µm FPP with more than half the backpressure.

A separation of ultra-short and long chain PFAS (C1-C18) is performed on a HALO®PCS Phenyl-Hexyl column along with a HALO®PFAS Delay column which demonstrates excellent retention for both hydrophilic and hydrophobic analytes.

Laboratories continually work to increase the capacity of their equipment, improve turnaround times and gain confident and detailed insights without generating additional burden on their operators. Discover how adopting the simplification strategy of Industry 4.0 with the GC 2400™ Platform can enhance GC workflows to increase efficiency, data quality, and business sustainability

Sustainability strategies are being integrated into a growing number of businesses and their operations. Laboratories are no exception. Although the necessity to reduce the environmental impact of laboratory operations is recognized, it cannot be disengaged from the economic viability of labs. This article shares ways in which laboratories can improve both their business and environmental sustainability and discusses how the GC 2400™ Platform is helping laboratories flourish in these areas

The potential of smart technologies has evolved into a operational necessity - businesses were faced with the need for remote and automated operations bringing substantial improvements in productivity, efficiency, and operating costs.

More and more regulations regarding PFAS are being implemented with action limits that continue to decrease. Having a harmonized method to accurately determine the PFAS content in food, as well as other matrices, is important to ensuring long-term detection and regulation. The solvent extraction of PFAS from these varied sample matrices can be challenging given the susceptibility to contamination and the low levels in which these compounds are present. This poster, presented at RAFA 2024, examines the PFAS extraction several different food samples as well as food packing matrices.

PFAS have been shown to cause health issues in humans, which means monitoring environmental solid samples, such as soil and tissue, is critical. This application note details the extraction of 40 spiked PFAS compounds from soil and tissue following EPA Method 1633. The automated extraction was less than 10 minutes per sample and yielded acceptable recoveries and RSDs without carryover in the system. The EDGE PFAS is an ideal option for laboratories that want to automate their PFAS extractions of solid samples.

Existing solvent extraction techniques are predominantly manual methods that are not rapid, simple, or efficient. The EDGE PFAS™, an automated solvent extraction system, provides an automated, sequential approach to PFAS extraction. This system performs an efficient extraction of PFAS compounds from challenging food and food packaging matrices in one simple process. Upon completion, the final extract is filtered and ready for any minimal cleanup required, as well as analysis. Excellent recovery with tight reproducibility has been found. The EDGE PFAS method offers a rapid, simple, and efficient solvent extraction solution for PFAS testing.

There are several established PFAS standard methods that specify the use of Liquid Chromatography/Mass Spectrometry (LC/MS); however, LC/MS is unable to analyze all PFAS compounds for various reasons. With its ability to analyze these compounds in complex matrices and simplified sample prep, Head-Space Solid Phase Microextraction GC/MS is pivotal as a complementary technique to LC/MS. Download this application for details on a method using GCMS for PFAS identification and quantitation.