Application Notes: General

Characterizing intact biotherapeutics such as monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) under physiological conditions is essential for understanding their critical quality attributes. This paper will examine several advanced chromatographic and detection solutions—including SEC, HIC, affinity chromatography, MALS, and HRMS—to support structural analysis, molecular weight determination, and DAR quantification.

The first charged aerosol detector (CAD) was introduced in 2005. Nearly twenty years later, this near-universal detector has become an indispensable tool in the analytical chemist’s quantitative arsenal. This paper explores the CAD’s origin story, its adoption into everyday use by separation scientists, and its comparison to evaporative light scattering detector (ELSD). Technical highlights will include how parameters such as evaporation temperature influence application versatility, and how the power function impacts linearity and response behavior.

Ultrashort-chain PFAS (C1–C4) are important due to their widespread presence and here is an application solution for this analysis.

Step inside the world of forensic investigations with “From Sample to Verdict,” a LCGC International digital e-book sponsored by Shimadzu. “From Sample to Verdict” highlights recent advancements in analytical techniques for forensic science, particularly involving chromatography and mass spectrometry.

Slalom chromatography separates nucleic acids >3 kbp in <6 minutes using shear-based molecular stretching for high-resolution, gel-free analysis.

he United States Pharmacopeia (USP) has designations for all columns stationary phases used in the monograph methods. These designations outline the stationary phase type, i.e. fully porous or solid-core, and any ligand attachments, i.e. C18 or Phenyl to be used.1 However, beyond that no column specifics are given. With a multitude of columns that fit into the different designations, understanding that not all columns are the same is vital when selecting a stationary phase for a monograph method. This application note examines three columns that all fit into the L1 designation when analyzing paracetamol impurities. Selectivity differences between the columns are considered in relation to the impurities.

Explore how wide-pore SEC delivers sharp, reproducible separation of megadalton-sized DNA vectors and plasmids with minimal secondary interactions.

VUV’s GC–VUV platform with ASTM D8267 enables rapid, automated measurement of total, mono-, and diaromatic content in jet fuel with superior precision and reproducibility compared to FIA and HPLC. This direct-injection method requires no sample prep or calibration curves, delivers results in 14 minutes, and significantly lowers cost per analysis

VUV’s GC–VUV platform with ASTM D8071 enables fast, automated PIONA and multi-parameter gasoline analysis in a single run. It delivers superior precision and reproducibility compared to traditional methods, requires no sample prep or calibration curves, and consolidates multiple regulatory tests into one cost-efficient workflow

VUV detection extends HPLC sensitivity into 150–290 nm, enabling direct detection of underivatized amino acids with higher signal and linearity than PDA, eliminating the need for derivatization

Demonstrate reliable measurement of water at trace levels and beyond in acetonitrile using gas chromatography (GC) combined with the LUMA Multi-Channel Vacuum Ultraviolet (VUV) Absorbance detector. Additionally, we will demonstrate the ability of LUMA to detect water and multiple solvents in a single analysis.

HPLC columns are the critical component of any SEC/GPC experiments. Selecting the right column, compatible with your mobile phase, temperature range, particle size, and pore size, is essential for accurate results. Using this Column Selection Guide simplifies this process, helping you choose the ideal column for your analysis with confidence.

This article demonstrates the suitability of ultrapure water from a Milli-Q® water system equipped with VOC-Pak® point-of-use cartridge for sensitive VOC analysis. This water is free of 58 VOCs evaluated, making it optimal for Purge and Trap GC-MS analysis of these compounds.

Polymers, generally referred to as plastics or resins, are used for a variety of purposes and have become deeply entrenched in modern life. As the petrochemical industry that produces polymers continues to grow, polymers with new structures and specialized functions continue to be developed and manufactured. Size exclusion chromatography (SEC) is a widely used technique for assessing the molecular mass of these polymers. This handbook introduces the basic principles of SEC, along with methods of analysis and examples of analysis of real samples.

GPC method development, optimization, and data interpretation can be complex and time-consuming. To support you in overcoming these challenges, we have compiled a collection of application notes covering the GPC analysis of a wide range of polymers. This application notebook provides practical insights, real-world examples, and proven methods to help you improve accuracy, streamline your workflow, and get the most from your GPC data.

When coupled with gel permeation/size exclusion chromatography, light scattering detection allows the measurement of true – or “absolute” – molecular weight (MW) of macromolecules, as opposed to the relative values obtained from conventional column calibration methods. The LenS3 MALS detector is a static multi-angle light scattering detector compatible with any UHPLC, HPLC, SEC, or GPC systems, designed to determine molecular weight and size within the range of 200-108 g/mol and 2-300 nm. This guide describes the technology, features, and functions of the LenS3 MALS Detector, along with application data examples. Learn how easy it is to utilize MALS in your polymer characterization.

Static light scattering detectors can be coupled to SEC/GPC systems for the determination of true molecular weight and size of polymers or biomolecules-independent of column calibration. This book is an essential resource for scientists and analysts using SEC or GPC, offering clear explanations of light scattering fundamentals, traditional methodologies, and the latest advancements in the field. Whether you're looking to deepen your understanding or improve your analytical accuracy, this guide offers both theory and practical insights.

As pharmaceutical laboratories face increasing pressure to improve efficiency, sustainability, and analytical precision, the demand for highly repeatable, resource-efficient instrumentation is on the rise. Capillary-scale liquid chromatography (LC) is reshaping expectations, enabling labs to achieve high-impact results without added overhead.

Identification of polyphenolic catechin compounds for health and industrial quantification and quality control of tea products.

A novel HIC column featuring innovative particle technology is benchmarked for separation of high DAR species ADCs, against a reference column with conventional chemistry.

PROTACs are protein degraders that harness the cell’s disposal machinery to remove specific targets. Now advancing into clinical trials, they show high potency at nanomolar levels. To support safe and effective drug development, sensitive and selective assays are essential for reliable detection and quantification.

Join us to see how SCIEX OS software helps you meet regulatory requirements with confidence. This webinar highlights new features and tools that strengthen data integrity, improve quality control, and save valuable lab time.

This infographic explores the benefits of Acoustic Ejection Mass Spectrometry (AEMS)- based workflows for efficient small and large molecule screening.

Metrohm’s MiPCT-ME technique enables trace-level determination of anions in N-methylpyrrolidone, ensuring lithium-ion battery quality, stability, and safety.

In this issue of “Hot Topics in Mass Spectrometry,” we highlight the role of ion mobility mass spectrometry (IM-MS) in addressing challenges in omics research, such as resolving isomeric ambiguity and enhancing sensitivity for low-abundance analytes, how selected ion flow tube mass spectrometry (SIFT-MS) is being used to conduct objective odor analysis in food and environmental samples, and the importance of sample preparation in quantitative bioanalysis.

We evaluated the accuracy of lipid quantitation in human plasma by LC-MS using single-point or multipoint calibration curves with authentic or surrogate standards.