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The vast majority of macromolecules exhibit a molar mass distribution that is often described by the polydispersity index (PDI). This Tips & Tricks instalment offers practical advice to consider when analyzing macromolecules using liquid chromatography (LC).

The degree and strength of self-association are critical quality attributes for insulin and its analogues. In this article we present the label-free determination of these quantities using composition-gradient multi-angle light scattering (CG-MALS), enabling the formulation of engineered insulin optimized for stability and efficacy.

A preview of the 8th International Symposium on the Separation and Characterization of Natural and Synthetic Macromolecules (SCM-8).

The ability of size-exclusion chromatography (SEC) to measure critical protein characteristics, such as molecular weight and size, makes the technique valuable from the early stages of novel protein research and development through to formulation and manufacturing support, especially in oligomeric purity and aggregation studies. However, informational output, ease of analysis, and sample requirements all vary considerably depending on whether a system has been truly optimized for protein characterization. This article examines how SEC works and considers how to maximize the productivity, sensitivity, and value of an SEC setup for biopharmaceutical development.

Selected highlights of innovative chromatography products.

This article looks at the practical benefits of microflow liquid chromatography–mass spectrometry (LC–MS) in bioanalysis applications.

Click the title above to open the LCGC Europe December 2016 regular issue, Vol 29, No 12, in an interactive PDF format.

Click the title above to open the LCGC North America December 2016 regular issue, Vol 34 No 12, in an interactive PDF format.

Click the title above to open The Column November 23, 2016 Europe & Asia issue, Volume 12, Number 21, in an interactive PDF format.

Click the title above to open The Column November 23, 2016 North American issue, Volume 12, Number 21, in an interactive PDF format.

This article looks at the practical benefits of microflow liquid chromatography–mass spectrometry (LC–MS) in bioanalysis applications.

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Research on nasal spray formulation has yielded an improved in vitro analytical test method utilizing thin layer chromatography (TLC). The research used simulated nasal mucus with varying properties and a TLC apparatus to assess the dripping behaviour of nasal formulations to improve the nasal spray development process.Nasal Spray Evaluation Using Thin Layer Chromatography

Rudolf Krska of the University of Natural Resources and Life Sciences (BOKU), in Vienna, Austria, has been awarded the biennial B.R.A.I.N award at the Biomin scientific conference held in Vancouver, Canada.

Phenomenex’s CEO Fasha Mahjoor has announced $12 million in bonuses for the company’s staff following the sale of Phenomenex Inc. to Danaher Corporation in October 2016.

Professor James Jorgenson of the University of North Carolina at Chapel Hill, in North Carolina, USA, has received the Csaba Horváth Medal and Scroll at the Csaba Horváth Memorial Award Symposium, Yale West Campus Conference Center.

Incognito defines the difference between a chromatographer and a chromatography user and elaborates on why we should care.

Although ultrahigh-pressure liquid chromatography (UHPLC) method development is fast and cost efficient, many of the analytical methods used in quality control laboratories are still conventional high performance LC (HPLC) separations. Transferring these HPLC methods to UHPLC and validating them is a time-consuming and labour-intensive task. Nevertheless, switching methods from HPLC to UHPLC equipment and vice versa is a powerful tool to increase laboratory efficiency. Modern systems with two flow lines in one system aim to simplify the transition between the two techniques.

Faster analyses, better separations, and lower consumption of sample and mobile phase are the primary drivers of size-exclusion chromatography with sub-2-µm beads and ultrahigh-pressures (UHP-SEC). The flip side of these benefits is higher sensitivity to column calibration errors and drift. There is also a relatively small selection of column chemistries available for eliminating non-ideal sample-column interactions. UHP-SEC can be combined with on-line multi-angle light scattering (UHP-SEC-MALS) to overcome these limitations and provide absolute molar mass and size of biomacromolecules, independently of retention time. UHP-SEC-MALS is also necessary for UHP-SEC characterization of proteins and biotherapeutics that have no appropriate reference standards, such as glycoproteins and PEGylated proteins.

As we enter the Generalization phase of the industrialization of Analytical Science, we find ourselves striving for the generic in as many areas as possible.