Gas Chromatography (GC)

For GC, how do data systems both assist and hinder us in obtaining maximum information from chromatograms? We explain how a chromatogram can provide a wealth of information about an individual analyte in a sample, about the sample itself, and about how well a GC instrument is performing.

Judicious initial choices of gas chromatography (GC) column dimensions, and even a change of column dimension during method development, can lead to significant improvements in resolution.

As we approach the holiday season, in what has a been the most challenging of years both inside and outside of the laboratory, I wanted to produce a more light-hearted yet inspiring review of 2020 within the Arch Sciences Group laboratories.

How can GC overcome the complexity of dioxin separation and analysis?

This instalment explores how the data system controls the functions of the GC instrument. Drawing on classical electronics and instrument designs, the article describes the evolution of instrument controls from knobs and gauges on the front panel of the instrument to computer control and current web-based systems.

Computers control all aspects of modern GC instrument operation, from temperature to valve actuation. We look under the hood to see how this works.

Capillary GC is renowned for being a ”high efficiency” technique, meaning that we typically see very narrow peaks within our chromatograms. This leads to the ability to separate many components in a reasonable amount of time, which is of course analytically advantageous.

In the present research, similar chromatography fingerprints were obtained using finely-tuned cryogenic-modulation (CM) and flow-modulation (FM) comprehensive two-dimensional gas chromatography–mass spectrometry (GC×GC–MS) experimental conditions.

Using the flame ionization detector (FID) as an example, we explain how the detector in a GC system generates a signal and how it is processed into chromatograms, and explore modern aspects of storing and processing digital data.

In gas chromatography, heating the sample in the inlet can lead to sample losses and loss of quantitative reproducibility, but these problems can be avoided using cold sample introduction. This article describes various types of cold injection and how they can benefit the analyst.

Optimizing gas chromatography (GC) separations typically involves making some informed choices around stationary-phase chemistry and column temperature programs

There are many potential causes of reduced peak size in gas chromatography (GC), and an inexperienced GC user may not know where to begin the troubleshooting process. Here, we review potential causes for reduced peak size in GC systems.

We are frequently asked about issues with reduced peak size in gas chromatography (GC), and I’m guessing this is related to just how difficult this problem is to troubleshoot. There are so many potential causes that an inexperienced GC user may not know where to begin the troubleshooting process. Fear not. What follows is our logical guide to locating and fixing the issues with loss of sensitivity, and we’ve tried to cover as many of the instrument and application issues that we can think of.

The Zebron ZB-PAH-EU and ZB-PAH-SeleCT offer unparalleled performance of targeted selectivity for PAHs in food, environmental, electronics, and fuel industries.

In this application, we look at purgeable volatile organic compounds by GC–MS using Zebron™ ZB-624PLUS™ GC columns.

In this application, we look at residual solvents per USP 467 using Zebron™ ZB-624PLUS™ and ZB-WAXPLUS™ GC columns.

How to reduce FAME run times by up to 75% and improve the separation of cis/trans FAME isomers while remaining compatible with AOAC, AOCS, and IOC regulatory methods.

In this application, we look at the fast analysis of alcohol-based sanitizers by GC–FID using Zebron™ ZB-WAXPLUS™ GC columns.

ZB-WAXplus offers performance nothing short of extraordinary – from 100 % aqueous stability to exceptional inertness.

Inert GC inlet liners for sample protection are easy to install. Touchless packaging provides protection from cuts or breakage, reducing contamination during installation process.

We look at the fast and accurate GC–MS analysis of polycyclic aromatic hydrocarbon (PAHs) in rubber and plastic using a Zebron™ ZB-PAH-EU GC column.

The ZB-DHA-PONA GC column has excellent response and peak symmetry for polar oxygenates - for DHA, PONA, PIONA, PIANO, ASTM methods (D5134, D5441, D5501, D6729, D6730, D6733).

In this application, we look at the determination of residual solvents and terpenes in cannabis by GC–FID using the Zebron™ ZB-624plus™ GC column.

Incognito observes some unexpected emerging trends at Pittcon 2020.

LCGC Europe sponsored the 2020 HTC Innovation Award to highlight innovation in separation science. The winner, Ryan Kelly, from Brigham Young University, in Utah, USA, has introduced an impressive array of innovative approaches to advance proteomics research using nano-liquid chromatography–mass spectrometry (nano‑LC–MS), LC–MS, and two‑dimensional (2D)-LC–MS, including the development and application of nanodroplet processing in one pot for trace samples (nanoPOTS). This platform, when combined with nano-LC–MS/MS, can identify more than 3000 protein groups in 10 cells, a greater level of proteome coverage than was previously possible for samples containing 5000 cells.