May 13th 2024
In this edition of The LCGC Blog, Katelynn Perrault and James Harynuk discuss the technological needs one faces when setting up a workstation for chromatography data analysis.
Validation in the Chromatography and Spectroscopy Laboratories
July 1st 2006Validation is one of the most critical issues facing today?s chromatographers and spectroscopists. With developments in this area moving for-ward at a staggering pace, it is more critical than ever that scientists have the most up-to-date information possible on validation.
Accurate Mass Compound Identification with Single-Quadrupole Gas Chromatography-Mass Spectrometry
June 30th 2006Gas chromatography-mass spectrometry using a single-quadrupole instrument is the workhorse technique of the environmental lab. It normally falls short for applications that require high mass accuracy. It is shown here that with proper calibration techniques, this technique can indeed readily obtain high mass accuracies to within a few millidaltons and become a powerful tool for unknown compound identification.
Validation in Biotechnology and Well-Characterized Biopharmaceutical Products
June 1st 2006This article describes the current situation in FDA-regulated areas, as well as characterization of these products. Finally,the author discusses the various stages of early- and late-phase product developments.
Stephen Dal Nogare: The Person Behind the Award
June 1st 2006The Stephen Dal Nogare award is one of the oldest and most prestigious awards given in chromatography. Little has been written about Stephen Dal Nogare "the man" or his contributions to scientific knowledge, including his unique contributions to separations science. This article describes his scientific career and how it has influenced the practice of chromatography.
Market Profile: Pharmaceutical Market Demand for Life Science Instrumentation
May 1st 2006Globally, 2005 sales of pharmaceuticals have been estimated at approximately $550 billion. A significant fraction of this amount was due to the top pharmaceutical companies in the world. The top five companies alone were responsible for total revenues of $168 billion, or 30% of the entire market.
The Rise and Fall of Expertise in Gas Chromatography
May 1st 2006Guest columnist Walter Jennings reflects on the early days of capillary gas chromatography (GC) and how chromatographers become experts in the technology by constructing their own columns, thereby achieving a more thorough understanding of the chromatographic process.
Chromatography for Bioanalytical Chemists
May 1st 2006This article looks at current practices in bioanalytical chemistry by examining and critically assessing the various parameters that can be altered to achieve high-speed results with high resolution in LC–MS applications. The decision to opt for gradient or isocratic elution is also discussed.
Novel Applications and New Developments in Gas Chromatography
March 14th 2006Exciting new uses for gas chromatography and its hyphenated forms show that there is an uncharted territory of applications and that there are limitations that still need to be overcome. The Tuesday afternoon oral session at Pittcon 2006, "GC-MS: Developments and Applications," gave the audience an idea of advances in GC and some new areas for the technique's application. John Chalmers of VS Consulting (Stokesley, United Kingdom) presided over the session.
Successful Gas Chromatography Using Fused-Silica Capillary Columns
March 1st 2006Capillary gas chromatography (GC) is a well-established separation technique. Rick Parmely reviews some of the capillary column basics by investigating a standard test mixture and observing some of the chromatographic effects that can affect peak response, peak shape and column bleed. He provides guidelines for obtaining the best performance from a capillary column. He discusses peak tailing, column overload, ghost peaks and column bleed and speculates on "when to give up." He concludes with a set of fundamental steps to be used in achieving better GC analyses.
Analysis of Volatile Bacterial Metabolites by Gas Chromatography–Mass Spectrometry
A method for the identification of key volatile organic compound (VOC) markers associated with infection by Neisseria meningitidis bacteria by gas chromatography–mass spectrometry (GC–MS) was developed. Headspace samples of bacterial VOCs were trapped on triple-sorbent bed tubes and then thermally desorbed into a laboratory GC–MS system for separation. Identification was carried out by comparison of GC retention time and electron ionization mass spectra to the National Institute of Standards and Technology (NIST) database. Further confirmation was obtained by GC–MS of known standard chemicals. A total of 75 VOCs were detected, five of which can be considered key VOC markers for Neisseria meningitidis. These peaks were identified as 1,2-dimethylcyclopropane, 2-methylpropanal, methacrolein, N-2-dimethyl-1-propanamine, and 3-methylbutanal by the NIST database.
Successful Gas Chromatography Using Fused-Silica Capillary Columns
November 1st 2005guest Columnist Rick Parmely takes a look at some capillary column basics of a well-established separation technique. He discusses peak tailing, column overload, ghost peaks, and column bleed, and speculates on "when to give up."
How To Buy Gas Calibration Mixes
October 1st 2005In January 2004, LCGC Europe published an article on methods for selecting pure gases for analytical applications. 1 Here, the authors expand on this topic by addressing questions regarding gas mixtures - defined simply as more than one gas in a cylinder.
Benchmarking for Analytical Methods: The Horwitz Curve
October 1st 2005Analytical chemists are concerned with the quality of their methods and results. An important question in this context is whether the precision of a newly developed and validated method is up to standard. In other words: is the precision of the newly developed method comparable to what could be expected? This article looks at how the Horwitz equation can answer this. It also describes the results of an extensive study involving 10000 laboratories which indicates that the relative reproducibility approximately doubles for every 100-fold decrease in concentration and that, surprisingly, it does not depend on the type of material or method.
Cost Beneficial Validation of a Site-wide Chromatography Data System
September 1st 2005The implementation and validation of a new site-wide chromatography data system for a major active pharmaceutical ingredient custom manufacturer provided the opportunity to map and optimize the GMP and ISO business processes to use electronic signatures effectively. This article describes the process optimization and how it integrates with the validation activities of the system. This overall approach provides substantial business benefits from the use of electronic signatures as evidenced by the savings resulting from process improvement and by the fact that the non-GMP laboratories implemented a similar process to analyse and approve results electronically.