HPLC

This article discusses useful approaches for the resolution of overlapping and superimposed peaks in HPLC.

A summary of new HPLC systems, modules, and software, focusing on innovations and unique features from a user's perspective.

Is it possible to get the same separation under isocratic and gradient conditions?

The first instalment of the Perspectives in Modern HPLC column highlights some of the new HPLC systems, accessories and related technology introduced at Pittcon this year.

A number of retention time issues can cause problems in HPLC. Here's advice on how to handle common ones, such as retention time drift, temperature variability, problems arising from column equilibration, and difficulties validating a method.

A novel, simple, rapid and effective method to determine pesticide residues in red wine samples is described.

Incognito found a 2D LC solution to a tricky separation problem but this technique did not meet the client's requirements. The magnanimous columnist admits to a rare defeat on this occasion but praises the thrill of seeking a solution to a perplexing method development problem. Could any readers suggest a more straightforward solution?

Click here to view the complete E-Separation Solutions newsletter from February 7, 2013

It is important to consider the influence of the sample matrix when setting up an analytical method

How to account for the sample matrix when developing a method

A description of how refractive index detectors work and some good practices to get the most out of this powerful detector

Two promising wide-pore stationary phases were recently introduced for the fast and high resolution separations of large biomoleculaes: the recently launched widepore 3.6?m superficially porous particle and the hybrid-type sub 2-?m fully porous particle. This article focuses on the achievable throughout and resolution for the characterization of biomolecules.

How can we tell if an LC method is working properly?

An improved liquid chromatography tandem mass spectrometry (LC–MS–MS) method for the screening and confirmation of clenbuterol in human urine in sports doping applications was developed and validated.

Quinine- and quinidine-derived anion-exchanger chiral stationary phases have proven to be versatile in enantiomer separation of acidic compounds in HPLC. In this article, the authors demonstrate their performance in specific HPLC applications involving enantiomer resolution and topoisomer separation.

How can we tell if a liquid chromatography method is working properly? How can a compendial method be adjusted to speed up the run time?

A preview of HPLC 2013.

The HPLC 2012 conference was abuzz with talk of "slip flow" following the talk given by Mary Wirth of Purdue University about her groundbreaking work involving protein column efficiency. In this interview, Wirth explains how “slip flow†works and what this discovery can contribute to the application of LC-MS to disease research.

Click here to view the complete E-Separation Solutions newsletter from October 11, 2012.

Click here to view the complete E-Separation Solutions newsletter from October 4, 2012.

Two promising wide-pore stationary phases were recently introduced for the fast and high-resolution separations of large biomolecules: the recently launched wide pore 3.6 ?m superficially porous particle and the hybrid-type sub-2-?m fully porous particle. This article focuses on the achievable throughput and resolution for the characterization of biomolecules.

How ethylene-bridged hybrid inorganic-organic (BEH) particles are prepared, and how their special quallities can be used to improve separations.

Some of the newer hydrophilic interaction liquid chromatography (HILIC) columns are reviewed, and experiments performed to categorize columns for optimized performance.

A discussion of new column packing materials– including sub-2-?m particles, superficially porous particles and second-generation monoliths– and their impact on instrumentation and laboratory productivity.

An overview of the principles and equipment required for scaling analytical separations to the preparative scale and considerations for the scale of analyte required.