HPLC

The results of a recent survery on stationary phases used in high performance liquid chromatography (HPLC) are revealed.

A mysterious autosampler problem is solved.

A systematic way to develop HPLC methods consists of building up and running a useful set of experiments (DOE, design of experiments) to evaluate the influence of several factors or method conditions on the selectivity of a chromatogram. The crucial part here is the peak tracking between the chromatograms.

Liquid chromatography is never immune to unexpected problems.

The 23rd International Symposium on Chiral Discrimination (Chirality 2011) was held in Liverpool, UK, this past July.

A look at situations in which retention times are too long, too short, or inconsistent

Phenazepam is becoming a drug of interest in forensic laboratories. This new procedure will enable forensic toxicologists to analyze phenazepam in biological fluids quickly and easily.

The practical aspects of hollow-fiber liquid-phase microextraction in the three-phase mode (HF3LPME) are described.

A discussion of how UHPLC is used to conduct intact protein–antibody analysis and glycoprofiling to characterize biopharmaceutical drugs

Janet Kelsey spoke to Stephen Luke, Product Manager at Thermo Fisher Scientific about the developments in HPLC columns and methods and the effect the growing tendency to use UHPLC has on the development of HPLC columns

Reversed-phase ultra high performance liquid chromatography (RP-UHPLC) columns are the most widely used UHPLC columns in analytical separations. As with other reversed-phase columns, analytes are separated in RP-UHPLC columns due to their differences in the degree of hydrophobic interaction with the stationary phase.

Too much variability in your liquid chromatography method?

Describes a new method to address the challenges of detection, analysis and characterization of low-abundant metabolites in metabolomic studies.

How to react to symptoms that indicate that your liquid chromatography column has major problems.

Too much variability in your liquid chromatography method?

Analysis of natural compounds is becoming increasingly important. One thriving area is the evaluation and application of environmental biopolymers, such as humic substances (HSs) in various applications, including medicine, industry, agriculture and environmental protection.

The optimum in proteomics research today is in validating the performance of promising biomarkers across large biological sample sets. Many new strategies have been developed in recent years to improve and accelerate targeted MS verification assays.

Liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) is often used for the identification of proteins from complex peptide mixtures made from enzymatic digests of selected proteomes.

Meeting review of HPLC 2011 held in Budapest, Hungary.

How to choose another column to try and solve a separation problem.

A novel approach for detection, analysis, and characterization of low-abundant herbicide metabolites is reported here.

Chrom Corner

A look at ways to estimate what normal system pressure should be and some likely causes of various pressure abnormalities

A method is presented for the determination of ?-amanitin, a toxic cyclic octapeptide of amanitin mushrooms, in human serum by solid-phase extraction coupled with high performance liquid chromatography with UV detection.

For method development or routine analysis the minimum gradient time may be a limit of throughput.