July 26th 2024
Scientists from Università Politecnica delle Marche reported on baseline separation of isotopologoues of some amphetamine (AMP) derivatives on achiral and polysaccharide-based chiral columns.
Scientists Reveal New Method for Soil Pesticide Detection Based on UHPLC-MS/MS
February 1st 2024Scientists in Mexico developed a new method for detecting herbicides in agricultural soil, being based on techniques like 9-fluorenylmethyl chloroformate (FMOC-Cl) and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).
Q&A: Unraveling the Dynamics of GM3 Gangliosides in Human Milk Across Lactation Stages
February 1st 2024Weronika Hewelt-Belka, an assistant professor at Gdańsk University of Technology in Poland, answers questions about her research analyzing GM3 gangliosides in human milk, elucidating their molecular distribution and dynamic changes throughout lactation.
HPLC Methods with Different Detection Types Determine Whey Protein Content in Cheese
February 1st 2024High performance liquid chromatography (HPLC) was paired with both diode array and fluorescence detection (DAD and FLD, respectively) to measure Edam cheese for its total and acid-soluble whey content.
HPLC-DAD Measures Phytochemical, In Vitro, and In Silico Properties of Polygonum cognatum Meissn
January 31st 2024This study of the perennial herb also known as madimak (or Indian knotgrass) aimed to simultaneously determine phenolic compounds to gain more insights into their antioxidant or antimicrobial capabilities.
Synthesizing Synthetic Oligonucleotides: An Interview with the CEO of Oligo Factory
January 15th 2024LCGC and Spectroscopy Editor Patrick Lavery spoke with Oligo Factory CEO Chris Boggess about the company’s recently attained compliance with Good Manufacturing Practice (GMP) International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) Expert Working Group (Q7) guidance and its distinction from Research Use Only (RUO) and International Organization for Standardization (ISO) 13485 designations.
pH-Responsive Deep Eutectic Solvent Enables Accurate Bisphenol A Detection in Water
January 12th 2024This experiment introduced a deep eutectic solvent developed in conjunction with a pH-induced phase transition combined with high performance liquid chromatography (HPLC) for efficient trace determination of bisphenol A (BPA) in water samples.
This review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
The Gradient Delay Volume, Part I: Theory
January 1st 2024The gradient delay volume is arguably one of the most important, yet least appreciated, parameters that affect how gradient elution separations in LC work. This has implications both for method development and for method transfer during the lifecycle of a LC method. In this installment, I will review the concept of gradient delay volume, its physical connection to the LC instrument, and how it can impact method development and separation quality.
Rising Stars of Separation Science: Zhuoheng Zhou
December 5th 2023This month we spoke to Zhuoheng Zhou from the Vrije Universiteit Brussel in Belgium about his research on advancing instrumental and column technologies for modern chromatography and its applications in novel biopharmaceutical modalities and proteomics.