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© 2020 MJH Life Sciences and Chromatography Online. All rights reserved.
© 2020 MJH Life Sciences™ and Chromatography Online. All rights reserved.
June 01, 2020
When you restart liquid chromatography (LC) instrumentation that was idle during the COVID-19 shutdown, you need to follow a systematic approach. Otherwise, problems may appear in days or weeks following startup.
May 01, 2020
There are still many methods in use that have been developed for use at “room temperature.” With such a method, can one reasonably expect to obtain the same separation in Anchorage, Alaska, as in Mumbai, India?
March 01, 2020
How do I know when bioinert liquid chromatography columns, systems, or components are needed for my separations of biomolecules?
December 01, 2019
A deeper theoretical understanding the relationship between peak area and flow rate will help analysts diagnose problems when using UV absorbance detection.
November 01, 2019
Many users have questions about how long LC columns should be re-equilibrated following solvent gradient elution separations. Here we show satisfactory results with short re-equilibration periods, for both reversed-phase and HILIC separations.
October 01, 2019
The authors provide a technical overview of the design and operating principles of variable wavelength and photodiode array detectors, and
include historical perspectives and common practices in operation and maintenance.
In this installment, tips, tricks, and suggestions are provided for best practices in reversed-phase separations, in both 1D and 2D LC, with gradient elution for minimum variations in retention time.
September 01, 2019
Carefully diluting a sample with weak solvent can mitigate the impact of sample solvent on peak shape in both reversed-phase and HILIC separations, but we need to understand how the choice of sample diluent can affect analyte recovery.
August 01, 2019
The high levels of non-volatile salts typically used in ion-exchange separations
are incompatible with mass spectrometry. But the desire to directly couple these two powerful techniques has prompted researchers find solutions to this problem. Here are three approaches.
We explain recent trends in HPLC autosampler design, provide recommendations for selecting one, and offer guidelines for operation and troubleshooting.