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Articles

This article discusses the main influences in oligonucleotide analysis using ion-pair (IP) reversed-phase chromatography. 

Improving Chromatographic Results for Oligonucleotides with Column Hardware

A column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.

Analysis of siRNA with Denaturing and Non-Denaturing Ion-Pair Reversed-Phase Liquid Chromatography Methods

In this first TRLC instalment, our recent developments, new possibilities, and current limitations of the use of 1D TRLC are discussed, while the column performance is described with respect to the fundamentals of HPLC. 

Perspectives in Hydrophobic Interaction Temperature-Responsive Liquid Chromatography

In this latest instalment of our series on recurring challenges in liquid chromatography (LC), we turn our attention to peak widths that are wider than expected. Estimating the expected peak widths can help determine when a column and LC system are not working quite right, and to decide when troubleshooting should be initiated.

Essentials of LC Troubleshooting, Part 6: How Wide Should Those Peaks Be?

This month we interview Martina Catani from the University of Ferrara, Italy, about her work on the separation of enantiomers by high performance liquid chromatography/ultrahigh-pressure liquid chromatography (HPLC/UHPLC) and the study of the kinetic and thermodynamic properties of chiral stationary phases, and the separation and purification using LC analysis (single-column and continuous multi-column) of biomolecules of high therapeutic interest.

Rising Stars of Separation Science:  Martina Catani

Webcasts

Webinar Date/Time: Thursday, February 16th, 2023 at 8am PST | 11am EST | 4pm GMT | 5 pm CET


20 Steps to a Reliable Mobile Phase

When considering different ways to improve an established LC method, it helps to start by reviewing some essential concepts.

What Are My Options to Improve My Separation? Part I: Foundational Concepts

Tony Taylor is Group Technical Director of Crawford Scientific Group and CHROMacademy. His background is in pharmaceutical R&D and polymer chemistry, but he has spent the past 20 years in training and consulting, working with Crawford Scientific Group clients to ensure they attain the very best analytical science possible. He has trained and consulted with thousands of analytical chemists globally and is passionate about professional development in separation science, developing CHROMacademy as a means to provide high-quality online education to analytical chemists. His current research interests include HPLC column selectivity codification, advanced automated sample preparation, and LCâMS and GCâMS for materials characterization, especially in the field of extractables and leachables analysis.

Robots outperform humans in five areas, and thus can be particularly beneficial for performing sample preparation for HPLC.

Viewpoints: The Rise of Automation—Evolve or Die?

A harmonization of all conventional hold-up volume methods was successfully applied to RPLC, HILIC, AEX, and RP-AEX mixed-mode chromatography, regardless of the mobile phase.

Harmonization of Experimental Methods Used to Measure the True Hold-Up Volume of Liquid Chromatography Columns

Developing a short list of the likely causes of these results can help streamline our troubleshooting experience when sensitivity-related problems occur.

Liquid Chromatography (LC/HPLC) | Topics