Application Notes: General

Shodex™ has SB-800 HQ series for the analysis of water-soluble molecules and for the determination of molecular weight distribution.

This technical article introduces an example of multi-faceted analysis and monitoring of an EV sample purification process using a SEC column, Shodex™ OHpak SB-806 HQ, with 3 different detectors.

When selecting a suitable analytical SEC column, not only the analytical range for the fractionation, but adsorption of the target compounds, which influences the quantification and yield, should also be considered.

mRNA coupled to EGFP (enhanced green fluorescent protein) is analyzed using a widepore YMC-Accura Triart Bio C4 RP column as well as a non-porous BioPro IEX QF column.

This application note explains how SEC coupled with MALS can be used for the determination of structural changes in polysaccharides.

This application note outlines an extraction method for four natural cannabinoids and two major metabolites of Δ9-THC in blood using Clean Screen® THC SPE column.

A robust analysis method for steroids was developed that can readily be implemented by clinical, forensic, or anti-doping laboratories.

This application note features a unique, simple, and robust solid-phase extraction method for cannabinoids in urine followed by analysis with LC–MS/MS.

This application note introduces a simple targeted extraction method for the analysis of nine nitazene compounds from urine and blood using the Clean Screen® DAU column.

Explore how a systematic screening strategy allows chromatographers to rapidly identify ideal method conditions.

This white paper explores the theory behind solid-core particles and the advantages they offer for small molecule analysis.

This article discusses the impact and theory of column temperature and how it can be a powerful tool for fine-tuning a separation during method development.

An increased number of samples demands increased throughput. This article discusses ways to achieve high throughput analysis with LC–MS analysis methods.

In LC separations, the majority of analytes are ionizable, so controlling the state the analytes are in is critical. Discover how to harness pH for method development.

As more gene therapy applications make inroads into research and clinical practice, the development and safe manufacturing of the therapeutic products involved have demanded more from associated analytical technologies. One of the most widely used gene-therapy vectors is AAV, and key technologies for the characterization of AAVs include CE and LC-MS.

This application note introduces some new application data obtained by using FlexFire SEC that is adopted by newly developed silica gel.

In this app note, a two-dimensional separation is conducted on an oxidative lignin depolymerization mixture using centrifugal partition chromatography.

Tecan’s proprietary NBE™ format enables digestion or incubation AND elution in a single SPE column. Read this app note to learn more and streamline your workflows!

This application note demonstrates analysis of fruit peels by performing DHS-GC-MS with a Pyroprobe 6200.

The fast identification of tocopherols are important for various applications including free radical scavenging for human health and food stuff preservation.

We demonstrate the utility of size-exclusion chromatography (SEC) for the detection of high-order structure of single-stranded DNA oligonucleotides.

The use of resistor tubing and 200 mM ammonium acetate in the mobile phase was implemented to measure loading capacity of a Biozen 1.8 μm dSEC-2 column.

Protein standards were explored with known hydrodynamic radii, showing how this can be used to determine ideal size exclusion conditions for optimal method performance.

Using standard phosphate containing buffers with different percentages of isopropanol for aggregate analysis of an antibody-drug conjugate (ADC), Kadcyla

Temperature, gradient program, and flow rates are explored and optimized for intact mass applications for mAbs when using Biozen WidePore C4 column with bioTi hardware.

The hydrophilic nature of the Biozen dSEC-2 stationary phase shows favorable aggregate profiles for forced degradation work under high salt conditions.

The Clarity OTX 2 mg/well format is used for the isolation, characterization, and quantitation of current antisense oligonucleotide therapeutics from small biological fluids.