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Separation Science: Past, Present, and Future

ByLaura Bush

LCGC North America

Milestone events, like LCGC's celebration of 30 years of covering separation science, always prompt reflection on the past and consideration of what the future might hold. Thus, for this 30th anniversary issue, we turned to the experts in five key areas of our coverage: sample preparation, gas chromatography (GC) columns, GC instrumentation, liquid chromatography (LC) columns, and LC instrumentation. Now, in the special group of five articles in this issue, we bring you their perspectives on the state of the art: what the most important recent advances have been, where things stand today, and where the field is likely to go next.

Understanding Ion-Exchange Chromatography

ByTony Taylor

LCGC North America

Ion-exchange chromatography (IEC) is based on the different affinities of analyte and eluent counterions for the oppositely charged ionic functional groups on the stationary-phase surface of an exchange resin. Depending on the charge of the surface electrostatic groups, the resin could be either an anion exchanger (positively charged stationary phase) or a cation exchanger (negatively charged stationary phase).

Can We Trust Experimental Standard Deviations?

ByVeronika R. Meyer

LCGC Europe

The statistical nature of experiments leads to the fact that standard deviation (SD) of replicate analysis is not constant but a number with some variability: a standard deviation has its own standard deviation. As a consequence, a SD calculated from three experiments is not rugged and even SDs from ten experiments show a great variability. Analysts should be aware that with 100 or more the results have low scatter

Agilent and Samsung Collaborate

LCGC Asia Pacific eNews

Agilent (Santa Clara, California, USA) will collaborate with the Samsung Medical Centre, one of South Korea's leading medical institutes, on medicine and genetics research projects.

Agilent and Samsung Collaborate

LCGC Europe eNews

Agilent (Santa Clara, California, USA) will collaborate with the Samsung Medical Centre, one of South Korea's leading medical institutes, on medicine and genetics research projects.

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Pharmaceutical Separations Technology Forum

LCGC Europe eNews

Experts from the pharmaceutical industry discuss current and emerging trends in detection techniques, the growing presence of UHPLC technologies and the impact of company consolidation. Participants in this forum include Simon Lomas, Brand Manager, HPLC and UHPLC products, Phenomenex, Frank Steiner, Manager, Small Molecule Solutions, Thermo Fisher Scientific and Dominic Moore, Senior Business Manager Pharmaceutical Business Operations, Waters Corporation.

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Redefining UHPLC and UHPLC/MS

LCGC Europe eNews

The new Thermo Scientific Dionex UltiMate 3000 XRS system—an extremely versatile solution for ultra-high resolution and high throughput UHPLC applications with 1250 bar and extreme low gradient delay volume.

LGC and HFL gear up for the Olympics

LCGC Asia Pacific eNews

HFL Sport Science (HFL), a part of the LGC Group (Teddington, Middlesex, UK) and one of the world’s largest sports drug surveillance laboratories, is preparing for its role testing the horses competing in equestrian events at the Olympic and Paralympic Games.

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New ACE® ExcelTM 2µm Ultra Robust UHPLC columns

LCGC Europe eNews

ACE Excel 2µm Ultra Robust UHPLC columns from Advanced Chromatography Technologies offer you all the benefits that chromatographers have come to expect from ACE columns (exceptional reproducibility and robustness, combined with excellent quality and efficiency) but have been especially engineered to withstand the demanding conditions of UHPLC.

Using LC-MS to Study Protein Expression in Crop Development

LCGC Europe eNews

As the need for improvements in the yield, insect resistance and abiotic stress tolerance of crop plants continues to increase, Gia C. Fazio and colleagues Wayne Skinner, Zina Dahmani, Yingzhi Lu and Jean C. Kridl have collaborated on a paper that describes the use of liquid chromatography–mass spectrometry (LC–MS) to quantify the expression of levels of a transgenic protein, barley alanine aminotransferase, across multiple rice lines.

LGC and HFL gear up for the Olympics

LCGC Europe eNews

HFL Sport Science (HFL), a part of the LGC Group (Teddington, Middlesex, UK) and one of the world’s largest sports drug surveillance laboratories, is preparing for its role testing the horses competing in equestrian events at the Olympic and Paralympic Games.

Agilent collaborates to identify causes of outbreaks

LCGC Europe eNews

Agilent (Santa Clara, California, USA), The U.S. Food and Drug Administration, the University of California, Davis, California, USA, and the Centres for Disease Control and Prevention have announced a collaboration to create a public database of 100,000 foodborne pathogen genomes to help speed identification of bacteria responsible for foodborne outbreaks.