In this edition of “Inside the Laboratory,” Betsy Stone, PhD, a professor of chemistry at the University of Iowa, discusses her group’s current research endeavors, including developing a new liquid chromatography–mass spectrometry (LC–MS) method to track secondary organic aerosol that forms in the atmosphere from D5.
The introduction of comprehensive two-dimensional gas chromatography (GC×GC) to the sample screening toolbox has substantially increased the ability to comprehensively characterize complex mixtures. However, for many gas chromatography (GC) users, the thought of having to learn to develop methods on a new technology is daunting. Developing a basic GC×GC method for most (nonspecialized) applications can be accomplished in minimal time and effort given parameter suggestions and ranges to target analytes in a sample of interest. In this article, the authors work describe a simple workflow to develop a GC×GC method for a specific sample upon initial use, with the aim of decreasing the time to accomplish functional workflows for new users.
Researchers built a reliable breath collection and analysis method using thermo-desorption gas chromatography-mass spectrometry (TD-GC–MS) that can produce a comprehensive list of known volatile organic compounds (VOCs) in the breath of a heterogeneous human population.
This year, the ISC’s theme is “Imagine a world of Chromatography." Here, we will see conference attendees discuss a wide variety of disciplines where chromatography plays a crucial role.
Petroleum contamination from leaking underground storage tanks, for example, is a significant concern for both the environment and human health. Thorough characterization of the contamination is required to form appropriate risk assessments and remediation strategies, but until now, the determination of total petroleum hydrocarbons (TPHs) in soil has typically involved a convoluted and labour-intensive process. In this article, the analysis of TPH in environmental media is simplified using flow-modulated GC×GC–FID with quantitation based on pre-defined compound groupings. This approach overcomes the drawbacks of conventional solvent fractionation approaches, by eliminating the need for sample fractionation and automating data processing workflows.
Column insertion distance is critical to good chromatography. What happens if the column is installed too low in the injection port? Is insertion distance more important when performing split injection or splitless injection? Does the position of the column in the injection port impact reproducibility?
A concise summary and celebration of the role of sample preparation for quantitative analysis in bioanalytical applications.
Researchers analyzed 31 different brands of hand sanitizers using headspace gas chromatography-mass spectrometry (GC–MS) to assess volatile impurities and ethanol content in ethanol-based hand sanitizers.
As data volumes and expectations for fast scientific discovery continue to increase, laboratory-based research organizations can no longer rely on a siloed approach to data management. To remain competitive, scientific organizations need to connect all their data, from discovery through manufacturing, in a unified informatics platform.
To gain insight into the effect of drying temperature on its composition, headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC–MS) was used for the identification of volatile organic compounds (VOCs) of 86 mushroom samples that were divided into five groups and dried at different temperatures.
A recent journal article provided an overview of a dataset that includes 10,000 experimental retention times, derived from 30 different reversed-phase liquid chromatography (LC) methods.
Researchers from the University of British Columbia have introduced an assay developed and validated to measure steroids in human primary teeth using liquid chromatography-tandem spectrometry (LC-MS/MS).
Click here to download the North America PDF of The Column September 2024 issue.
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Plasma samples from 47 patients with stages 1– 4 chronic kidney disease were analyzed with liquid chromatography-mass spectrometry to identify early biomarkers of the disease’s progression through metabolic pathway analysis.
A multi-faceted approach consisting of targeted quantitation, chemical profiling, and suspect screening via ultra-high performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) determined the environmental and health risks stemming from crumb rubber recycled from end-of-life tires and repurposed for a variety of uses, including artificial turf fields.
A recent study of 56 Italian ciders utilizing comprehensive two-dimensional gas chromatography coupled to time-of-flight-mass spectrometry (GC×GC–TOF-MS) detected the presence of approximately 130 semi-volatile organic compounds.
A recent study analyzed nearly 100 CBD products to test the accuracy of advertised cannabinoid content.
In a new study, researchers explored the dynamics of immune gene expression, ruminal metabolome, and gut microbiota in cows.
At HTC-18 in Leuven, Executive Editor of LCGC International, Alasdair Matheson, spoke to Brett Paull from the University of Tasmania and HyTECH about his talk entitled: “Analytical Performance and Disruptive Potential of Fully Portable Portable and Field Deployable LC–MS Technology.”
Using nanoflow liquid chromatography electrospray ionization in tandem with mass spectrometry (LC-ESI-MS), researchers were able to assess baleen whale blubber and proteins extracted from that blubber.
The Agricultural Biotechnology Research Institute of Iran (Tabriz, Iran) recently utilized gas chromatography-mass spectrometry (GC-MS) to explore the impact of different animal sources and regions in Iran on the metabolome profiles of traditional yogurts.
A recent study used liquid chromatography tandem-mass spectrometry (LC-MS/MS) for a targeted proteomics analysis to establish cerebrospinal fluid-based proteomic panels for accurate primary central nervous system lymphoma diagnosis and differentiation.
A recent report from Frost & Sullivan predicts that the expanding use of chromatography in pharmaceuticals, especially in drug development and clinical research, coupled with the food and beverage industry’s heavy reliance on chromatography for quality control, is expected to accelerate market expansion to $6.60 billion in 2028.
LCGC International recently asked analytical scientists in industry and academia to share how mentorship has impacted their careers.
The editors of LCGC International explore how chemists can set themselves apart in an ever-competitive job market, highlighting the latest data in salary and employment statistics.
The article discusses the classification and characterization of GC×GC column sets based on polarity and orthogonality, introducing a standardized approach using a reference mixture called the Century Mix to evaluate the selectivity and performance of these column sets.
In this article, we discuss the fundamentals of headspace extraction, including static versus dynamic extraction, establishing equilibrium in the vial, consequences of the partition coefficient, temperature, pressure, and transfer to the gas chromatograph.
A rapid, accurate, and quantitative method for the analysis of glucosinolates (GSLs) has been achieved through the combining of column-free liquid chromatography (LC) with direct-infusion mass spectrometry (DIMS).
Recent research utilizing tandem mass spectrometry has yielded a complete set of expressed globulin genes, which code for the main storage protein in oats as well as their chromosomal positions.
