Biopharmaceuticals and Protein Analysis

Countercurrent chromatography systems were tested and combined to optimize their capacity for handling non-polar and polar substances.

A new study done by scientists in Switzerland and Sweden investigated the use of automated systems to develop multispecific antibodies.

To create a technique suited for analyzing proanthocyanidins and their properties, scientists combined high-speed countercurrent chromatography (HSCCC) and electrospray ionization–tandem mass spectrometry (ESI–MS/MS).

Circular dichroism and fluorescence were used to create a platform for monitoring changes in monoclonal antibodies.

Researchers tested immobilized metal affinity chromatography’s potential for broad applications.

LCGC International interviewed Professor Torgny Fornstedt, Professor in Analytical Chemistry & Leader of the Fundamental Separation Science Group at Karlstad, Sweden at HPLC 2025 in Bruges, Belgium.

LCGC International interviewed Torgny Fornstedt, Professor in Analytical Chemistry & Leader of the Fundamental Separation Science Group at Karlstad University, Sweden at HPLC 2025 in Bruges, Belgium on modern separation methods to analyse oligonucleotides.

Bo Zhang, associate professor at Xiamen University, China, spoke to LCGC International at HPLC 2025 in Bruges, Belgium, about his recent research on a novel elastic connection technology—macroporous polyacrylamide hydrogel septum (MAPS)—which was developed as a complementary solution for capillary column interfacing in liquid chromatography.

Using two-dimensional liquid chromatography–mass spectrometry, a new workflow was developed to characterize phosphorodiamidate morpholino oligomers, which can help treat infectious diseases.

Recently, we spoke with Esther Olonimoyo from the University of Maryland, who recently published a study using high-performance liquid chromatography to analyze short chain fatty acids.

To better detect polycyclic aromatic hydrocarbons in the human body, a UHPLC–MS/MS method was developed and tested on urine samples from workers exposed to diesel exhaust.

To quantify antibiotics in ICU patients’ blood, dried blood spot analysis was sued alongside UHPLC–MS/MS to monitor these substances in a more convenient matter.

In a new study, researchers used GC–MS and LC–MS were used to detect composition fraud in food supplement creation.

Organic contaminant transformations in water were researched to understand the mechanisms behind such phenomena.

A gas chromatography–mass spectrometry-based approach was created and optimized to analyze reaction products of partial isobutane oxidation.

Researchers from Waters and Biospring studied the contribution of nucleotide type and modifications on the retention and resolution of 22–24 nt long oligonucleotides in different chromatographic methods.

Capillary gas electrophoresis was used recently to study what optimizes messenger RNA analysis.

Hydrophobic interaction chromatography (HIC) is useful, albeit costly, for purifying proteins. Researchers aimed to reformulate HIC isotherms using salt-dependent water activity to optimize the process.

Shea kernels contain bioactive secondary metabolites that can offer potential health benefits. Researchers aimed to learn more about these substances.

In the latest edition of "LC Troubleshooting", Dwight Stoll explores troubleshooting tips for liquid chromatography, particularly focusing on peak width challenges in large molecule separations and effective solutions.

To meet the increasing worldwide demand for monoclonal antibodies, it is crucial to develop purification methods that are both efficient and easily scalable. Membrane chromatography offers a promising solution for streamlining antibody purification, thanks to its ability to handle high processing volumes rapidly. Convective membrane chromatography has become popular for purifying monoclonal antibodies. Researchers created a new model for modeling mass transfer during purification.

To address multi-drug resistance (MDR) among harmful substances, liquid chromatography–tandem mass spectrometry was used to test fosfomycin as a means of bypassing growing MDR amongst microorganisms.

Detecting tryptophan and other metabolites can be done using various techniques. Researchers created a new UHPLC–MS/MS-based method for quantifying these substances in human plasma.

HIV-based virus-like particles can be difficult to characterize in samples. Recently, researchers from institutions in Germany enhanced anion-exchange chromatography procedures to better detect and treat these substances.

Here is some of the most popular content posted on LCGC International this week.