Biopharmaceuticals and Protein Analysis

Curcumin is a polyphenol believed to have anticancer effects. To better quantify its existence in plasma, reversed-phase high-performance liquid chromatography (RP-HPLC) was used to create a new analytical approach for quantifying this substance.

In this article, you’ll find some of the top content that was published on LCGC this week, including calls for new chromatography technologies and interviews with rising stars in the field.

To create a new approach for monitoring and quantifying free fatty acids (FFAs), Chinese researchers combined stable isotope derivatization coupled with liquid chromatography–triple quadrupole mass spectrometry (ID-LC-QQQ-MS).

Enantiomer self-disproportionation can occur during various scientific processes. To understand temperature’s relationship with this phenomenon, researchers studied how temperature affected SDE in chromatographic separation.

Researchers from Comenius University Bratislava used LC–MS/MS to analyze albendazole substances in plasma samples.

Researchers at the Institute of Marine Research in Bergen, Norway used liquid chromatography–mass spectrometry to analyze resolvins in fish and human cells.

A new approach for purifying immunoglobulin-based therapies was created by Pfizer researchers.

To detect popular party drugs in human waste, researchers combined headspace solid-phase microextraction and solid-phase microextraction to detect these substances and their metabolites.

University of Geneva researchers led a collaboration for testing different size-exclusion chromatography columns to compare the capability for characterizing mRNA.

Researchers at LUT University highlight challenges in calculating adsorption values during aqueous chromatography and explore new methods to close this knowledge gap.

To better analyze polyphenols, scientists from Sapienza University of Rome examined the capabilities of supercritical fluid chromatography (SFC), comparing it to traditional methods.

Countercurrent chromatography systems were tested and combined to optimize their capacity for handling non-polar and polar substances.

A new study done by scientists in Switzerland and Sweden investigated the use of automated systems to develop multispecific antibodies.

To create a technique suited for analyzing proanthocyanidins and their properties, scientists combined high-speed countercurrent chromatography (HSCCC) and electrospray ionization–tandem mass spectrometry (ESI–MS/MS).

Circular dichroism and fluorescence were used to create a platform for monitoring changes in monoclonal antibodies.

Researchers tested immobilized metal affinity chromatography’s potential for broad applications.

LCGC International interviewed Professor Torgny Fornstedt, Professor in Analytical Chemistry & Leader of the Fundamental Separation Science Group at Karlstad, Sweden at HPLC 2025 in Bruges, Belgium.

LCGC International interviewed Torgny Fornstedt, Professor in Analytical Chemistry & Leader of the Fundamental Separation Science Group at Karlstad University, Sweden at HPLC 2025 in Bruges, Belgium on modern separation methods to analyse oligonucleotides.

Bo Zhang, associate professor at Xiamen University, China, spoke to LCGC International at HPLC 2025 in Bruges, Belgium, about his recent research on a novel elastic connection technology—macroporous polyacrylamide hydrogel septum (MAPS)—which was developed as a complementary solution for capillary column interfacing in liquid chromatography.

Using two-dimensional liquid chromatography–mass spectrometry, a new workflow was developed to characterize phosphorodiamidate morpholino oligomers, which can help treat infectious diseases.

Recently, we spoke with Esther Olonimoyo from the University of Maryland, who recently published a study using high-performance liquid chromatography to analyze short chain fatty acids.

To better detect polycyclic aromatic hydrocarbons in the human body, a UHPLC–MS/MS method was developed and tested on urine samples from workers exposed to diesel exhaust.

To quantify antibiotics in ICU patients’ blood, dried blood spot analysis was sued alongside UHPLC–MS/MS to monitor these substances in a more convenient matter.

In a new study, researchers used GC–MS and LC–MS were used to detect composition fraud in food supplement creation.

Organic contaminant transformations in water were researched to understand the mechanisms behind such phenomena.