Biopharmaceuticals and Protein Analysis

Recently, we spoke with Esther Olonimoyo from the University of Maryland, who recently published a study using high-performance liquid chromatography to analyze short chain fatty acids.

To better detect polycyclic aromatic hydrocarbons in the human body, a UHPLC–MS/MS method was developed and tested on urine samples from workers exposed to diesel exhaust.

To quantify antibiotics in ICU patients’ blood, dried blood spot analysis was sued alongside UHPLC–MS/MS to monitor these substances in a more convenient matter.

In a new study, researchers used GC–MS and LC–MS were used to detect composition fraud in food supplement creation.

Organic contaminant transformations in water were researched to understand the mechanisms behind such phenomena.

A gas chromatography–mass spectrometry-based approach was created and optimized to analyze reaction products of partial isobutane oxidation.

Researchers from Waters and Biospring studied the contribution of nucleotide type and modifications on the retention and resolution of 22–24 nt long oligonucleotides in different chromatographic methods.

Capillary gas electrophoresis was used recently to study what optimizes messenger RNA analysis.

Hydrophobic interaction chromatography (HIC) is useful, albeit costly, for purifying proteins. Researchers aimed to reformulate HIC isotherms using salt-dependent water activity to optimize the process.

Shea kernels contain bioactive secondary metabolites that can offer potential health benefits. Researchers aimed to learn more about these substances.

In the latest edition of "LC Troubleshooting", Dwight Stoll explores troubleshooting tips for liquid chromatography, particularly focusing on peak width challenges in large molecule separations and effective solutions.

To meet the increasing worldwide demand for monoclonal antibodies, it is crucial to develop purification methods that are both efficient and easily scalable. Membrane chromatography offers a promising solution for streamlining antibody purification, thanks to its ability to handle high processing volumes rapidly. Convective membrane chromatography has become popular for purifying monoclonal antibodies. Researchers created a new model for modeling mass transfer during purification.

To address multi-drug resistance (MDR) among harmful substances, liquid chromatography–tandem mass spectrometry was used to test fosfomycin as a means of bypassing growing MDR amongst microorganisms.

Detecting tryptophan and other metabolites can be done using various techniques. Researchers created a new UHPLC–MS/MS-based method for quantifying these substances in human plasma.

HIV-based virus-like particles can be difficult to characterize in samples. Recently, researchers from institutions in Germany enhanced anion-exchange chromatography procedures to better detect and treat these substances.

Here is some of the most popular content posted on LCGC International this week.

High-performance liquid chromatography (HPLC) was recently used to detect acylated impurities in artificial hormones meant to treat prostate cancer symptoms.

AI-powered tools are enhancing precision, efficiency, and decision-making in biopharmaceutical development. Recently, Jared Auclair and Anurag Rathore explored AI's evolving role in biopharmaceuticals in detail.

Bacteriophages are vital for addressing antibiotic-resistant bacteria, though phage therapies are currently rare. University of Lisbon researchers recently created a new purification workflow to address these concerns.

Separating stereoisomers is an extensively researched and challenging process, with approved substances being rare. A new SFC-based system was made to possibly ease some aspects of these procedures.

This study highlights the promising potential of LC–MS as a powerful tool for mAb quality control within the context of upstream processing.

Ceftazidime and avibactam are two anti-infective drugs that can prove difficult to analyze in serum. Determining them can assist in therapeutic drug monitoring or pharmacokinetic studies.

Highly reactive aldehydes can pose significant health concerns in polluted environments. HPLC was used in this study to simultaneously analyze carbonyl compounds hydrazones.

Texas Tech University researchers used liquid chromatography–tandem mass spectrometry to quantify glucose transporter 1 (GLUT1) in mammalian brain cells.

A recent roundtable discussion featuring Aloka Srinivasan of Raaha, Mayank Bhanti of the United States Pharmacopeia (USP), and Amber Burch of Purisys discussed the challenges surrounding nitrosamine analysis in pharmaceuticals.