Biopharmaceuticals and Protein Analysis

During the 18th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology, experts presented a workshop on different ways multidimensional liquid chromatography can be applied.

A recent paper discusses advances in hydrophilic interaction chromatography (HILIC) in the analysis of therapeutic oligonucleotides and their impurities.

Scientists from Changchun University of Chinese Medicine used UHPLC-Q-Orbitrap-MS/MS analysis to identify the active compounds in TongFu XieXia Decoction for treating intestinal obstruction.

Scientists from the Lunan Pharmaceutical Group explored the use of UHPLC-MS/MS to test the effects of Shouhui Tongbian capsules (SHTBs) on slow transit constipation (STC).

Scientists from Manipal Academy of Higher Education in India recently discussed different protein profile pattern analysis techniques and how they can help allow for universal screening.

Scientists from Vanderbilt University recently tested a new means of imaging mass spectrometry (IMS) for isotopically resolved intact proteins.

Scientists from Chongqing University Cancer Hospital recently developed a new system for analyzing the potential impact of retention time (RT) prediction on targeted LC–MS-based lipidomics.

Scientists from the Chinese Academy of Sciences recently tested out a new type of micro gas chromatographic column (µGCC) for separating light hydrocarbons.

Scientists from Florida State University used Marfey’s reagent and reversed-phase liquid chromatography–tandem mass spectrometry (LC–MS/MS) to analyze D-amino acids.

Michelle Colgrave of Edith Cowan University and Australia’s national science agency is the recipient of the 2024 Harvey W. Wiley Award.

Swedish and German scientists recently made a new system for detecting of selective androgen receptor modulators (SARMs) in human samples.

Scientists from the University of Córdoba in Córdoba, Spain recently used liquid chromatography–tandem mass spectrometry (LC–MS/MS) to comprehensively profile human ceramides to determine their reactions to diseases.

Here are the top five articles that the editors of LCGC International published this week.

In this edition of the LCGC Blog, Jonathan Edelman discusses the 43rd Annual International Symposium and Exhibit on the Separation, Purification, and Characterization of Biologically Important Molecules (ISPPP) and provides a timeline for the upcoming conference.

The 26th Norwegian Symposium on Chromatography was held 21–23 January 2024. The symposium has strong traditions in the Norwegian separation science community, serving as a forum for excellent scientific talks, networking, and social events.

Scientists from Auburn University recently investigated how natural deep eutectic solvents (NADES) can be incorporated into high-performance liquid chromatography (HPLC) mobile phases.

Scientists recently tested a new system for detecting and extracting favipiravir from plasma samples.

This article explores the key challenges analytics and mass spectrometry solve within the biopharmaceutical industry.

A recent study conducted protein analysis using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and a platform that utilizes copper oxide particles.

Scientists from Western Michigan University tested how amino acids can affect protein stability during electrothermal supercharging.

A review of advances in mRNA analysis using LC and LC–MS.

Biopharmaceutical analysis is a rapidly evolving field that requires the development of new technologies and methods to keep pace with the increasing complexity of biologics. One of the most promising areas of research is the use of single-cell omics and microfluidic chips for the analysis of biopharmaceuticals. Single-cell omics has revolutionized our understanding of cellular heterogeneity, while microfluidic chips have enabled high-throughput analysis of single cells that provide an understanding of the complex biological network that complements the genomics and transcriptomics studies. This article will explore some of the emerging trends and technologies in biopharmaceutical analysis, with a particular focus on single-cell omics and microfluidic chips. We will also discuss the developments in ambient ionization mass spectrometry such as sub nanoampere ionization and the potential of low current ionization in studying cell-to-cell heterogeneity and its role in metabolomics.

Scientists from Fudan University in Shanghai, China created a system for reducing retention-time variations in metabolomic analysis using ultrahigh-performance liquid-chromatography coupled with mass spectrometry (UPLC-MS).

An introduction from our guest editors.

Innovative native separation strategies coupled with native mass spectrometry techniques to characterize biopharmaceuticals close to their natural state are described.

The authors discuss key findings from recent biosimilarity assessments they conducted on biosimilars of granulocyte-colony-stimulating factor (G-CSF), insulin glargine, rituximab, and trastuzumab.

Researchers explore the benefits of a mobile affinity selection chromatography (MASC) luminon assay method to assess the quality of therapeutic monoclonal antibodies.

Scientists recently used laser desorption ionization mass spectrometry (LDI-MS) to detect toxic alkaloids in traditional Chinese medicines (TCM).

Two scientists from Baskent University in Ankara, Turkey analyzed multiple proteins in cream formulation using reverse-phase high-performance liquid chromatography.

Scientists from Zhejiang, China recently conducted experiments meant to properly characterize sucrose ester isomers from tobacco samples.