Biopharmaceuticals and Protein Analysis

An overview of different approaches for the qualitative and quantitative analysis of polysorbates.

In this interview, we explore the significance of this groundbreaking technology and its impact on mass spectrometry with Aaron Robitaille, Director of Marketing for Mass Spectrometry at Thermo Fisher Scientific in San Jose, California.

During the North American Chemical Residue Workshop, scientists discussed predicting liquid chromatographic retention times to identify contaminants.

Researchers compare the separation efficiency of olefins and paraffins using stationary phases containing silver(I) ions in ionic liquid (IL) and polymeric ionic liquid (PIL) matrices for gas chromatography techniques.

Researchers have introduced a novel primary method utilizing gas chromatography–isotope dilution infrared spectrometry (GC–IDIR) for accurate peptide purity analysis.

Researchers have developed a robust method utilizing high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) to accurately quantify amino acids in plasma, making it a valuable tool for clinical diagnostics and research in amino acid metabolism.

Scientists from the Pacific Northwest National Laboratory are using high-resolution ion mobility spectrometry-mass spectrometry to identify unknown compounds.

Researchers have discovered that phospholipids form doubly charged lipid-metal ion complexes during electrospray ionization (ESI), providing valuable insights into the analysis of phospholipid structures.

A research team has developed a new approach for selecting precursor ions in tandem mass spectrometry (MS/MS) experiments called DiffN, which increases the likelihood of biomarker discovery and provides a promising tool for lipidomic analyses.

New research reports on the development of a groundbreaking strategy that utilizes metal oxide-nanochannel arrays as templates for the in-situ growth of metal-organic frameworks (MOFs), revolutionizing the field of protein isolation, offering promising potential for high-efficiency protein recovery from complex biological samples.

In Alejandro Cifuentes's keynote lecture at HPLC 2023, he discussed the research he and his team have done to explore how foodomics can be effective in combating Alzheimer's disease.

A new study reveals that real-time library searching significantly improves the structural characterization of glycerophospholipids and sphingomyelins, offering enhanced selectivity and comprehensive analysis. By utilizing real-time identifications to trigger targeted MSn analyses, researchers gain deeper insights into these crucial lipid species.

Researchers have discovered diacylglycerols as potent biomarkers for the rapid diagnosis of breast cancer, using mass spectrometry imaging. This breakthrough study offers new possibilities for improved intraoperative assessment and personalized treatment strategies.

Scientists have developed a breakthrough MDLC–MS method for in-depth characterization of charge variants in therapeutic antibodies. The innovative approach enables the detection and evaluation of rare charge variants, facilitating improved safety and efficacy in antibody development.

The development of a newly-designed Girard's reagent, HBP, as charged isobaric mass tags has enabled selective capture and sensitive detection of aldehyde metabolites. The LC–dNLS-MS/MS screening approach using this reagent has successfully facilitated the discovery of natural aldehydes and their profiling in complex samples.

A temperature-controlled cryogenic distillation and gas chromatography, atom trap measurement system is used for precise analysis of atmospheric 85Kr abundance. This advanced technology enables online monitoring with high sensitivity and reduced sample size requirements.

Characterizing lipid nanoparticles (LNPs) is a developing delivery modality in biotherapeutic analysis. We address some of the current challenges and opportunities in this field, including an examination of the most common tools used to characterize LNPs.

Researchers in Iran utilize graphene oxide aerogels to significantly improve the extraction and analysis of risperidone from plasma samples, offering enhanced accuracy and efficiency in pharmaceutical analysis.

A new publication introduces a groundbreaking automated system for high-throughput mass spectrometry analysis, reshaping the future of biotherapeutic characterization.

A recent study has introduced comprehensive quality control procedures for large-scale plasma proteomics analyses. The research provides valuable insights into optimizing performance, enhancing reproducibility, and ensuring quantitative accuracy in proteomics investigations.

A novel analytical method combines charge inversion ion-ion reactions with ion mobility–mass spectrometry to enhance the separation and characterization of oligosaccharides.

A new tandem mass spectrometry technique has been developed for the rapid determination of complex lipid A structures, including novel variations from arctic bacterioplankton. This study enhances our understanding of bacterial adaptations and paves the way for the development of targeted therapies and antimicrobial agents.

A cutting-edge technique combining droplet microfluidics and mass spectrometry revolutionizes monoclonal antibody screening, enabling rapid and high-throughput analysis while setting a new standard for efficient clone selection in biopharmaceutical development.

Automation and high-throughput MALDI mass spectrometry revolutionize peptide metabolic stability screening, allowing for rapid analysis of a large number of samples and minimizing solvent waste.

A team of researchers have created an innovative analytical platform combining asymmetrical flow field-flow fractionation (AF4) with native mass spectrometry (nMS) to characterize non-covalent protein complexes, providing valuable insights into their stability and higher-order structures.