Biopharmaceuticals and Protein Analysis

Scientists have developed a breakthrough MDLC–MS method for in-depth characterization of charge variants in therapeutic antibodies. The innovative approach enables the detection and evaluation of rare charge variants, facilitating improved safety and efficacy in antibody development.

The development of a newly-designed Girard's reagent, HBP, as charged isobaric mass tags has enabled selective capture and sensitive detection of aldehyde metabolites. The LC–dNLS-MS/MS screening approach using this reagent has successfully facilitated the discovery of natural aldehydes and their profiling in complex samples.

A temperature-controlled cryogenic distillation and gas chromatography, atom trap measurement system is used for precise analysis of atmospheric 85Kr abundance. This advanced technology enables online monitoring with high sensitivity and reduced sample size requirements.

Characterizing lipid nanoparticles (LNPs) is a developing delivery modality in biotherapeutic analysis. We address some of the current challenges and opportunities in this field, including an examination of the most common tools used to characterize LNPs.

Researchers in Iran utilize graphene oxide aerogels to significantly improve the extraction and analysis of risperidone from plasma samples, offering enhanced accuracy and efficiency in pharmaceutical analysis.

A new publication introduces a groundbreaking automated system for high-throughput mass spectrometry analysis, reshaping the future of biotherapeutic characterization.

A recent study has introduced comprehensive quality control procedures for large-scale plasma proteomics analyses. The research provides valuable insights into optimizing performance, enhancing reproducibility, and ensuring quantitative accuracy in proteomics investigations.

A novel analytical method combines charge inversion ion-ion reactions with ion mobility–mass spectrometry to enhance the separation and characterization of oligosaccharides.

A new tandem mass spectrometry technique has been developed for the rapid determination of complex lipid A structures, including novel variations from arctic bacterioplankton. This study enhances our understanding of bacterial adaptations and paves the way for the development of targeted therapies and antimicrobial agents.

A cutting-edge technique combining droplet microfluidics and mass spectrometry revolutionizes monoclonal antibody screening, enabling rapid and high-throughput analysis while setting a new standard for efficient clone selection in biopharmaceutical development.

Automation and high-throughput MALDI mass spectrometry revolutionize peptide metabolic stability screening, allowing for rapid analysis of a large number of samples and minimizing solvent waste.

A team of researchers have created an innovative analytical platform combining asymmetrical flow field-flow fractionation (AF4) with native mass spectrometry (nMS) to characterize non-covalent protein complexes, providing valuable insights into their stability and higher-order structures.

Researchers have developed a novel liquid chromatography–mass spectrometry method for analyzing sialic acid linkage in N-linked glycopeptides, offering insights into glycosylation patterns.

Scientists have developed an automatic and highly efficient method to authenticate single herbal powders using microscopy-guided in situ auto-sampling combined with matrix-assisted laser desorption–ionization mass spectrometry (MALDI–MS).

New statistical framework developed by researchers at the University of California in Riverside allows confident identification of isomeric molecules by mass spectrometry, even when no unique mass-to-charge peaks are generated between the isomeric pairs.

The evolution of biomolecule analysis is discussed in this article.

A new article discusses the benefits and drawbacks of RPLC and RPLC×RPLC chromatography techniques. It provides recommendations for selecting the best approach for analyzing small pharmaceuticals and peptides.

A recent study analyzed the composition and metabolism of steroidal alkaloids in Solanum nigrum fruits of different maturities and identified steroidal glycoalkaloids and steroidal alkaloids, providing a foundation for further research on biosynthesis and discovery of new steroidal alkaloid anticancer drugs.

A new published study introduces a simple and fast way to differentiate between animal species based on their hair. The in-sample digestion method coupled with mass spectrometry analysis was successfully tested on multiple species.

A new study has identified potential quality-markers for Eucommiae Folium (Duzhongye), a traditional Chinese medicine, using UHPLC–MS/MS analysis, and has recommended isoquercitrin as a new quality-marker candidate.

Researchers at Clemson University have developed a computational framework that integrates commercial software components to perform customizable technoeconomic feasibility analyses of monoclonal antibody capture chromatography platforms.

Researchers combine a single-probe single cell mass spectrometry technique with a bioinformatics software package to quantify cell subpopulations and prioritize metabolite biomarkers, providing a label-free approach to studying cell heterogeneity and improving understanding of cell metabolism in human diseases and response to therapy.

Researchers have developed a PAMAM-functionalized paper substrate that boosts the sensitivity of protein analysis by paper spray mass spectrometry (PS-MS), potentially revolutionizing clinical diagnosis of diseases.

This article compares the performance of wide-pore silica monolithic, sub-2µm FPP, and SPP columns, addresses the question of whether 1000 Å or 400 Å SPP columns are more suitable for reversed-phase LC-type protein separations, and presents a kinetic performance comparison of different columns.

A micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.