HPLC

For the first time, inhibitors of a cyclooxygenase isoform were found in Allium ampeloprasum L., specifically the giant or “elephant” garlic grown in the Chiloé Archipelago.

At EAS 2023, Michael Dong and Szabolcs Fekete reviewed certain innovations in high performance liquid chromatography (HPLC), and in what ways scientists can continue to improve the quality of their experiments.

Scientists from Egypt have developed a green reversed-phase high-performance liquid chromatography (RP-HPLC) method to determine the amounts of methionine and paracetamol in different mixtures.

An efficient solvent system screening approach that streamlines fractionation of compounds with similar UV absorption eliminates the need for individual partition coefficient calculations, and coupled with preparative HPLC purification, led to the isolation of seven c-glycosyl flavonoids and an amino acid from barley seedlings.

Scientists from Ames National Laboratory in Ames, Iowa recently analyzed silver(I) and copper(I) ions to understand the environmental factors when they are altered.

We provide a high-level overview of Current Good Manufacturing Practice (cGMP) regulations and their impact on the analytical chemist in the pharmaceutical industry.

A team of scientists from Nanjing, China, have created a low-cost efficient online derivatization high-performance liquid chromatography (HPLC)-ultraviolet (UV) detector system, which they used to detect mono- and oligo-saccharides.

This month we interview Zhuoheng Zhou from the Vrije Universiteit Brussel in Belgium, about his focus on advancing instrumental and column technologies for modern chromatography and its applications in novel biopharmaceutical modalities and proteomics. We also spoke to him about his work developing a protocol to help with the design of polymer monolithic capillary columns.

In a recent study, a novel chiral column, based on a CC19-R porous organic cage, promises to enhance enantioselective HPLC separations.

Researchers have developed a meticulous method for analyzing impurities in a product used for early treatment of breast cancer in premenopausal and perimenopausal women, shedding light on the product’s degradation pathways.

A comparison between two types of detectors—variable wavelength (VWD) and fluorescence (FLD)—in quantifying azoxystrobin from water samples was conducted.

Researchers have developed a method for the simultaneous determination of betulinic acid, oleanolic acid, and ursolic acid in jujube extract, utilizing high performance liquid chromatography with ultraviolet detection (HPLC–UV) coupled with multilayer perceptron networks, offering a more efficient alternative to classical HPLC techniques.

Researchers have developed a highly sensitive and cost-effective HPLC-UV method for simultaneously detecting 36 D/L-amino acids in foods, opening new avenues for food quality control and shedding light on the significance of D-amino acids in nature.

A new ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) was used to detect purine metabolites in rat plasma, revealing more information about their role in several biological disorders.

Mobile phase degassing has become very useful for troubleshooting problems with pumps and detection.

GLP helps ensure the quality of different studies during drug development. Here, the authors outline practices relevant to analytical and bioanalytical chemists.

As chromatography technology evolves, our use of it should change also. This article outlines a number of protective and troubleshooting methods for LC and how these approaches have changed over time.

An overview of GLP regulations and related public standards.

In a recent study, researchers from China presented a novel approach that uses a supramolecular solvent (SUPRAS) method for detecting bioactive compounds.

This review of pharmaceutical analysis in the last three years includes three categories of chiral liquid chromatography stationary phases: saccharides, macrocyclic substances, and porous organic materials.

Researchers from Hebei University in China have developed and characterized three novel peptide chiral stationary phases (CSPs) for enantiomeric separation, demonstrating their potential in high performance liquid chromatography (HPLC).

Small modifications to the way measurements are approached can enable reductions in the waste generated and carbon dioxide produced during an analysis.

The author outlines multiple protective and troubleshooting methods for liquid chromatography and how these approaches have changed over time.

This retrospective focuses on trends and topics that were discussed at the annual HPLC symposium in Duesseldorf, Germany.

Understanding the relationship between a number of variables and analysis time, and their effects on other choices made during method development, is helpful for developing methods that are both effective and time-efficient.