HPLC

This article explores what this transition to newer technology looks like, with an example applied to determining organic impurities of pramipexole dihydrochloride.

Several factors strongly influence separation speed, including the pressure available to drive the separation, column temperature, particle size, and column length. Developing efficient methods to improve speed while not sacrificing accuracy require an understanding of the abovementioned variables and analysis time.

Nonspecific adsorption leads to poor peak shapes and poor performance from HPLC systems, but coating-enabling technologies can serve as a potential solution. We explain how.

What you need to know about pharmaceutical regulations as an analytical chemist is provided here, including an explanation about how working in a Good Manufacturing Practice (GMP) environment can yield positive results.

Are analytical chemists really operating sustainable laboratories, or are they being too quick in marketing their “green” laboratory credentials?

This study suggests a 2D-LC screening method to evaluate peak purity and ensure specificity in pharmaceutical analysis, thereby promoting the safe production of medicines by detecting active pharmaceutical ingredients (API) and related substances.

Nonspecific adsorption leads to poor peak shapes and poor performance from HPLC systems, but coating-enabling technologies can serve as a potential solution. We explain how.

Superficially porous particle (SPP) column technology is leveraged to optimize high-throughput HPLC methods for small-molecule drug discovery, resulting in improved efficiency and reduced runtime.

Researchers in Iran utilize graphene oxide aerogels to significantly improve the extraction and analysis of risperidone from plasma samples, offering enhanced accuracy and efficiency in pharmaceutical analysis.

A novel system combining hydrogel-modified human umbilical vein endothelial cell membrane chromatography with high-performance liquid chromatography-mass spectrometry revolutionizes the screening and purification of active ingredients in herbal medicines.

Researchers have developed a novel liquid chromatography–mass spectrometry method for analyzing sialic acid linkage in N-linked glycopeptides, offering insights into glycosylation patterns.

We interviewed an AI program (ChatGPT) for LCGC North America asking questions about AI and its role in various applications for separation science to include data analysis, and high performance liquid chromatography (HPLC), hydrophilic-interaction chromatography (HILIC), reversed-phase liquid chromatography (RPLC), liquid chromatography–mass spectrometry (LC–MS), gas chromatography–mass spectrometry (GC–MS), high resolution mass spectrometry (HRIM–MS), high resolution tandem mass spectrometry (HRMS/MS), and related topics.

The evolution of biomolecule analysis is discussed in this article.

The 51st International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2023) will be held at the Conference Center Düsseldorf (CCD) in Germany, from 18–22 June 2023.

A newly proposed concept in liquid chromatography (LC) positions spherical particles, either individually or stacked, in pockets forming microgrooves that act as perfectly aligned chromatographic columns.

A new study has identified potential quality-markers for Eucommiae Folium (Duzhongye), a traditional Chinese medicine, using UHPLC–MS/MS analysis, and has recommended isoquercitrin as a new quality-marker candidate.

Trace amounts of polychlorinated biphenyls (PCBs) in a river and lake in China were detected using a custom coating for stir bar sorptive extraction (SBSE) followed by high performance liquid chromatography with diode array detection (HPLC-DAD).

The reliability of column chromatography for GC–MS analysis of hopanes was tested in oil samples of three different types, using neutral alumina as a solid-phase adsorbent and a Pasteur pipette as a separation device.

Researchers have developed a new approach to extract chloramphenicol (CAP) from milk and chicken samples using magnetic nanofluid-based deep eutectic solvents (DES), which was combined with high performance liquid chromatography-ultraviolet (HPLC-UV) analysis. The method was found to be low-cost, green, efficient, and effective in detecting even trace amounts of CAP.

Scientists have developed a magnetic ionic liquid hypercrosslinked polymer composite for use in magnetic solid-phase extraction (MSPE) of non-steroidal anti-inflammatory drugs (NSAIDs), which was coupled with high-performance liquid chromatography (HPLC) for the detection of trace amounts of NSAIDs in water and urine samples.

This article compares the performance of wide-pore silica monolithic, sub-2µm FPP, and SPP columns, addresses the question of whether 1000 Å or 400 Å SPP columns are more suitable for reversed-phase LC-type protein separations, and presents a kinetic performance comparison of different columns.

A micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.

This article highlights an advantage of porous graphitic carbon columns, that is their resistance at high temperatures, for the analysis of parabens in foodstuffs.

Researchers have developed a new method for detecting pyrethroid pesticide residues in tobacco using Schiff base networks (SNWs) coated stir bar sorptive extraction (SBSE) coupled with high performance liquid chromatography-ultraviolet detection (HPLC-UV), which exhibited high extraction efficiency and good mechanical stability even when reused more than 50 times.

High performance liquid chromatography–mass spectrometry and gas chromatography–mass spectrometry, along with numerous extraction methods, are helping to find organosulfur compounds in food more easily, providing researchers better information about the health benefits of these substances.

What are the options available when adjusting selectivity (peak spacing or elution pattern) when dealing with relatively simple mixtures?

Exploring the impact of green chemistry on analytical chemistry and GC is the focus of this instalment of "GC Connections".

The combination of an untargeted approach using ultrahigh pressure liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF) and a targeted approach using UHPLC–tandem mass spectrometry (MS/MS) are presented as an ideal method for detecting per- and polyfluroalkyl substances (PFAS) in fast-food packaging.

Everyone is talking about sustainability, and organizations are creating sustainability programs. But what does green chemistry really mean, and how does it apply to gas chromatography?

Researchers have shown the effectiveness of ion chromatography (IC) in identifying markers of forest fire emissions in rainfall samples, providing valuable insights into the signature of forest fire emissions and aiding in the development of models to predict and control air pollution resulting from these events.