January 10th 2024
In this LCGC Blog, the author explores stationary phases that incorporate permanent or induced charges within, or alongside, hydrophobic alkyl silica type bonded ligands.
December 14th 2023
You're invited to publicize your 2023–2024 new product introductions in LCGC. Here's how to get your products included.
December 8th 2023
A group of scientists from Xi’an, China have created a new system for analyzing cell membranes based around HALO-tag technology.
December 5th 2023
A novel analytical method for the analysis of OLED materials using SFC is presented.
December 1st 2023
This article provides a high-level overview of Current Good Manufacturing Practice (cGMP) regulations and related public quality standards.
Rising Stars of Separation Science: Zhuoheng Zhou
This month we interview Zhuoheng Zhou from the Vrije Universiteit Brussel in Belgium, about his focus on advancing instrumental and column technologies for modern chromatography and its applications in novel biopharmaceutical modalities and proteomics. We also spoke to him about his work developing a protocol to help with the design of polymer monolithic capillary columns.
Innovative Chiral Column Boosts Enantioselective HPLC Separations
In a recent study, a novel chiral column, based on a CC19-R porous organic cage, promises to enhance enantioselective HPLC separations.
HPLC and LC–MS Estimation of Goserelin Acetate Sustained-Release Implant Impurity
Researchers have developed a meticulous method for analyzing impurities in a product used for early treatment of breast cancer in premenopausal and perimenopausal women, shedding light on the product’s degradation pathways.
Quantification of Azoxystrobin in Water Using a Variable Wavelength and Fluorescence Detectors
A comparison between two types of detectors—variable wavelength (VWD) and fluorescence (FLD)—in quantifying azoxystrobin from water samples was conducted.
MLP–HPLC–UV Method Simultaneously Determines Three Triterpenic Acids in Jujube Fruit
Researchers have developed a method for the simultaneous determination of betulinic acid, oleanolic acid, and ursolic acid in jujube extract, utilizing high performance liquid chromatography with ultraviolet detection (HPLC–UV) coupled with multilayer perceptron networks, offering a more efficient alternative to classical HPLC techniques.
New HPLC-UV Method Detects Amino Acids in Foods with High Precision
Researchers have developed a highly sensitive and cost-effective HPLC-UV method for simultaneously detecting 36 D/L-amino acids in foods, opening new avenues for food quality control and shedding light on the significance of D-amino acids in nature.
UHPLC-MS Method Used to Detect Purine Metabolites and Study Severe Biological Disorders
A new ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) was used to detect purine metabolites in rat plasma, revealing more information about their role in several biological disorders.
The Evolution of LC Troubleshooting: Degassing
Mobile phase degassing has become very useful for troubleshooting problems with pumps and detection.
Good Laboratory Practice (GLP): An Overview for the Analytical Chemist
GLP helps ensure the quality of different studies during drug development. Here, the authors outline practices relevant to analytical and bioanalytical chemists.
Evolution of LC Troubleshooting: Protective Devices and Column Repair
As chromatography technology evolves, our use of it should change also. This article outlines a number of protective and troubleshooting methods for LC and how these approaches have changed over time.
Good Laboratory Practice: An Overview for the Analytical Chemist
An overview of GLP regulations and related public standards.
Supramolecular Solvent Method Enhances Herbal Compound Analysis Using HPLC–MS/MS
In a recent study, researchers from China presented a novel approach that uses a supramolecular solvent (SUPRAS) method for detecting bioactive compounds.
Recent Chiral Stationary Phase Advancements in HPLC Analysis
This review of pharmaceutical analysis in the last three years includes three categories of chiral liquid chromatography stationary phases: saccharides, macrocyclic substances, and porous organic materials.
Newly Devised Peptide Chiral Stationary Phases Evaluated for Enantioselectivity in HPLC Applications
Researchers from Hebei University in China have developed and characterized three novel peptide chiral stationary phases (CSPs) for enantiomeric separation, demonstrating their potential in high performance liquid chromatography (HPLC).
The Paradox of Sustainability in Separation Science
Small modifications to the way measurements are approached can enable reductions in the waste generated and carbon dioxide produced during an analysis.
The Evolution of LC Troubleshooting: Protective Devices and Column Repair
The author outlines multiple protective and troubleshooting methods for liquid chromatography and how these approaches have changed over time.
Highlights from the 51st International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2023)
This retrospective focuses on trends and topics that were discussed at the annual HPLC symposium in Duesseldorf, Germany.
What Are Options to Improve My Separation? Part 4: Solutions to Consider for Improving Separation Speed
Understanding the relationship between a number of variables and analysis time, and their effects on other choices made during method development, is helpful for developing methods that are both effective and time-efficient.
Validation of an Aqueous Normal Phase Chromatography Method for the Analysis of Ergothioneine in Commercial Mushrooms
The authors developed a new analytical HPLC method using a silica hydride-based column to analyze mushrooms.
Highlights from the 51st International Symposium on High Performance Liquid Phase Separations and Related Techniques
This instalment of “Column Watch” presents many of the highlighted topics and trends observed at this exceptionally well-run symposium.
Functional Copolymers Analysis Using Liquid Chromatography (LC)—Sort It Out
The latest developments and challenges of using liquid chromatography (LC) for copolymer analysis are discussed.
FDA Selects New Mobile HPLC System for Cannabis Analysis
Orange Photonics said the agency will use its LightLab 3 as part of a broader effort to regulate and test the safety of food-related cannabis products.
Review of Packed Sorbent Solid-Phase Extraction and Microextraction Methods From Manual to Automated
Studies published over the last five years have focused on fully or semi-automated approaches and their applications in extraction of both organic and inorganic pollutants, while modern robotic tools and 3D printing are starting to make impacts.
Extracting Hypothalamic Peptides with A New Magnetic Approach
HRPs, while vital to creating drugs meant to fight serious diseases, are very difficult to quantify. To streamline the process, a group of scientists used a new approach for analyzing HRPs using MSPE and HPLC-UV.
Strongly Adsorbing Analytes: What, Why, and How to Fix It
We present two cases where understanding the specific interactions between particular analyte functional groups and LC instruments and columns is critical for successful separations.
Polysorbate Analysis in Biopharmaceutical Applications—A Snapshot of the Analytical Toolbox
An overview of different approaches for the qualitative and quantitative analysis of polysorbates.
HPLC Market Predicted to Reach $7.9 Billion by 2030
A new report from Meticulous Research attributes the growth to the development of antibody-based therapeutics and biosimilars.
New Method Enables Online Measurement of Tetraethyllead in Aqueous Samples
A research team has developed a new method for the online measurement of tetraethyllead (TEL) in aqueous samples. The approach utilizes magnetism-enhanced in-tube solid phase microextraction (ME/IT-SPME) coupled with high performance liquid chromatography (HPLC) and diode array detection (DAD).
Solvent-Based Extraction for Lignan HPLC Analysis in Forsythiae Fructus
Forsythiae fructus is a traditional Chinese medicinal herb that has been used to treat various ailments such as inflammation, fever, and infections.
HPLC 2023 Opening Ceremony Highlights
LCGC was on the ground at HPLC 2023.
2 Commerce Drive Cranbury, NJ 08512