HPLC

Governments are striving to implement policy changes towards a greater use of green technology, specifically around the generation of energy. Individuals and industrial organizations have also taken up the challenge, and now many companies are driving to significantly reduce their environmental footprint, or indeed become carbon negative within very short time frames.

An unexpected retention order for ketamine analogs was observed when using a biphenyl stationary phase for liquid chromatography-mass spectrometry (LC–MS).

In this edition of The LCGC Blog, Emanuela Gionfriddo discusses the two world-class scientists and trailblazer women in separation chemistry and the awards they received at Pittcon 2024.

Tony Edge, Stephane Moreau, Carsten Paul, and Fraser McLeod discuss the latest trends and developments in LC.

How long should you perform an extraction of woodruff leaves so that you don’t end up with a harmful amount of coumarin, but still get the characteristic flavor of the finished May wine?

LCGC’s Senior Technical Editor uses artificial intelligence (AI) platform ChatGPT to answer questions about the role of AI in separation science. Do you agree with ChatGPT?

Here are the top five articles that the editors of LCGC International published this week.

The flowmeter, Testa said in a press release, was compared favorably as an alternative to more traditional flow calibration methods for HPLC pumps.

Scientists from Wuhan Textile University recently tested a new solid-phase extraction system for analyzing covalent organic frameworks (COFs).

Scientists from the Shenyang Pharmaceutical University in China have developed a new quality assessment system for various types of traditional Chinese medicine (TCM).

In this installment, we apply the concepts developed in last month’s installment by demonstrating how they can be used to help troubleshoot problems in LC involving pressure and flow.

This article covers liquid chromatography columns and accessories commercially released after Pittcon 2023 through the 2024 conference.

A report estimates that the market for high performance liquid chromatography equipment will grow at a 4.61% CAGR between 2023 and 2033.

A recent study used ultrahigh-pressure liquid chromatography (UHPLC) to identify traces of drugs on smartphones.

As LCGC International celebrates Earth Day, we spotlight the current chromatographic research that is advancing environmental analysis and sustainability efforts. In this interview, we spoke to Daniel Armstrong, professor of chemistry and biochemistry at the University of Texas at Arlington, about his work using mathematical strategies to minimize analytical method greenness scores (AMGS) in HPLC enantioseparations.

In this interview, Angela Calderón of Auburn University discusses the importance of using more environmentally friendly analysis techniques.

Chromatographic and spectroscopic techniques alike were reviewed for their current effectiveness and future potential in this lecture session at Analytica 2024.

This article describes newly introduced high-performance liquid chromatography (HPLC), mass spectrometry (MS), chromatography data systems (CDS), and related products in 2023–2024. It summarizes their technical specifications and significant user benefits.

In the present study, a gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method has been designed and validated to quantify ornidazole (OZ) in the marketed formulation (oral gel) with the application of QbD.

The 26th Norwegian Symposium on Chromatography was held 21–23 January 2024. The symposium has strong traditions in the Norwegian separation science community, serving as a forum for excellent scientific talks, networking, and social events.

Scientists from Auburn University recently investigated how natural deep eutectic solvents (NADES) can be incorporated into high-performance liquid chromatography (HPLC) mobile phases.

Scientists recently tested a new system for detecting and extracting favipiravir from plasma samples.

This week, LCGC International published a variety of articles on the hottest topics in chromatography and beyond. Below, we’ve highlighted some of the most popular articles, according to our readers. Happy reading!

A recent study examined using high performance liquid chromatography coupled to mass spectrometry (HPLC–MS/MS) to optimize this key class of drugs that are used for the treatment of the common cold and flu.

A look at what’s in store for chromatographers who attend HPLC 2024 in Denver, Colorado, USA from 20–25 July 2024.

A recent study examined using high performance liquid chromatography (HPLC) with inductively coupled plasma–tandem mass spectrometry (ICP-MS) to detect ethylene-thiourea (ETU) and propylene-thiourea (PTU) in fruits and vegetables.

At Pittcon 2024, LCGC International editor Patrick Lavery sat down with Martina Catani of the University of Ferrara to talk about the work her laboratory group is conducting.

The concept of gradient delay volume (GDV) in liquid chromatography (LC) poses challenges for both beginners and experienced practitioners. The GDV, which affects the arrival time of mobile phase composition changes at the column inlet, can have a significant impact on method throughput, influencing the time required for mobile phase changes at both the beginning and end of the LC method. Different pump designs and column characteristics affect efficient use of the available analysis time, as well as overall throughput. Notably, achieving repeatable equilibration, rather than full equilibration of LC columns following mobile phase gradients, is often sufficient for many LC applications, which can also be leveraged to increase method throughput.

This article describes a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the analysis of coumarin in various tobacco matrices and electronic cigarette (E-cig) liquids, and highlights the importance of evaluating different MS/MS transitions of an analyte in complex sample matrices to overcome matrix effects. Matrix interfering components were separated from analyte using a C18 ultrahigh-pressure liquid chromatography (UHPLC) column with a larger inner diameter (3.0 mm, or 4.6 mm). Matrix suppressions on analyte responses were corrected by isotope dilution. Four different MS/MS transitions of coumarin were studied in each sample matrix to select a suitable MS/MS transition for analyte quantification based on matrix effects on each MS/MS transition. The method was validated using different tobacco matrices and E-cig liquids.

Scientists from Murcia, Spain recently created a new method for mycotoxin screening in human tissues using dispersive liquid–liquid microextraction and liquid chromatography–mass spectrometry.