January 10th 2024
In this LCGC Blog, the author explores stationary phases that incorporate permanent or induced charges within, or alongside, hydrophobic alkyl silica type bonded ligands.
December 14th 2023
You're invited to publicize your 2023–2024 new product introductions in LCGC. Here's how to get your products included.
December 8th 2023
A group of scientists from Xi’an, China have created a new system for analyzing cell membranes based around HALO-tag technology.
December 5th 2023
A novel analytical method for the analysis of OLED materials using SFC is presented.
December 1st 2023
This article provides a high-level overview of Current Good Manufacturing Practice (cGMP) regulations and related public quality standards.
Deployable Mass Spectrometry for Rapid On-Site Bioanalysis
With the advent of ambient ionization and portable mass spectrometers, the ability to perform rapid, on-site analysis is fast becoming a reality. We review the critical recent developments enabling this capability as well as remaining challenges that must be tackled to enable widespread adoption.
Whence the Next Generation of Macromolecular Separations Scientists?
Developing and building a talent base in macromolecular separations should be a priority for employers. Here’s why.
Pursuing the Polar Routes with Penguins and Polar Bears: Balancing the Choice of Chromatographic Mode
Using Earth as a figurative analogy, we explore the merits of flexibility when considering which liquid chromatography (LC) modality to use for any particular bioanalysis.
Essentials of LC Troubleshooting, Part III: Those Peaks Don’t Look Right
We explain several of the more frequent causes of bad peak shapes in liquid chromatography and provide tips on how to remedy them.
Column Overload in PLOT Columns
Knowing the variables that most affect the sample loading capacity in a porous layer open tubular (PLOT) column is vital to determining how much sample to inject.
The Pharmaceutical Industry and the Separation Scientist: Perspectives, Trends, and Career Opportunities
We kick off a new article series that offers an insider’s view of the pharmaceutical industry, focusing on the role of separation science and separation scientists.
Recent Developments in HPLC and UHPLC: From Our Guest Editor
In this issue, we feature fundamental and innovative efforts from within the walls of some the leading companies in the separation science industry. The theme was inspired by the continuous output of essential separation tools and the seemingly endless streams of pertinent information that these companies provide the scientific community.
An Assessment of Stationary Phase Selectivity in SFC
Sustainability concerns are renewing interest in SFC, often as a substitute for HPLC. With the broader application of SFC, we need a better understanding of selectivity in SFC, where stationary phase classification is not well established. This study assessed and measured the selectivity differences afforded by three prototype SFC phases.
Developing a Fast Purification Method for a Natural Product with a Preparative LC Column Packed with Superficially Porous Particles
Superficially porous particles (SPPs) are routinely used in analytical liquid chromatography (LC) columns, but they also provide benefits for preparative LC columns for use in pharmaceutical discovery laboratories and high-throughput environments. In this study, a preparative method using SPP-packed column had a 45% decrease in run time over a method using columns packed with fully porous particles, at standard flow.
Modifying the Metal Surfaces in HPLC Systems and Columns to Prevent Analyte Adsorption and Other Deleterious Effects
A novel surface modification technology has been developed to reduce interactions between analytes and metal surfaces in HPLC instruments and columns. We demonstrate the impact of this technology on peak symmetry, peak area, and injection-to-injection and column-to-column reproducibility for several metal-sensitive analytes.
Dealing with Wandering First-Dimension Peaks in 2D-LC Separations
Retention time fluctuations are challenging to deal with, especially in two-dimensional liquid chromatography (2D-LC) separations. Here, two approaches are presented that can help navigate this challenge.
New Liquid Chromatography Columns and Accessories in 2022
Our yearly report on liquid chromatography (LC) columns and accessories commercially released over the past year.
Cracking the Code of Complex Drug Modalities via Multidimensional Liquid Chromatography Coupled to Mass Spectrometry
Multidimensional liquid chromatography (MDLC) methods have revolutionized the characterization of complex drug modalities like antibodies–drug conjugates, antisense oligonucleotides, and small interfering RNA therapeutics.
Essentials of LC Troubleshooting, Part II: Misbehaving Retention Times
Developing a short list of likely causes of retention-time problems in reversed-phase LC makes troubleshooting easier.
New HPLC, MS, and CDS Products Introduced in 2021–2022: A Brief Review
Our annual review of new high performance liquid chromatography instruments, mass spectrometry systems, and data processing software.
Gaining New Insights in Advanced Polymeric Materials Using Comprehensive Two-Dimensional Liquid Chromatography
Two-dimensional LC, with advanced modulation techniques, can advance the analysis of advanced polymeric materials, assisting in characterizing copolymer composition heterogeneity and identifying ingredients in complex products.
But Why Doesn’t It Get Better? Kinetic Plots for Liquid Chromatography, Part III: Pulling It All Together
With kinetic plots, you can make better-informed column choices. Here’s how.
Critical Evaluation of Chromatography Methods: Essential Detective Skills
A little preparation eases the challenges of new methods—and increases the fun.
A Suitable Therapeutic Drug Monitoring Method for Amoxicillin in Plasma by High Performance Liquid Chromatography–UV (HPLC–UV) in Neonates
This simple, rapid, and accurate HPLC-UV method can facilitate routine therapeutic drug monitoring in neonates.
Improving Chromatography for Analysis of Biological and Pharmaceutical Molecules: Barry L. Karger, the 2022 winner of the Lifetime Achievement in Chromatography Award
Barry L Karger is Director Emeritus of the Barnett Institute, and is the James L. Waters Emeritus Chair and Distinguished Professor Emeritus at Northeastern University in Boston.
But Why Doesn’t It Get Better? Kinetic Plots for Liquid Chromatography, Part II: Making and Interpreting the Plots
A kinetic plot is a powerful tool, but how do you construct one—from either experimental data or data from other sources? We explain.
The Current Status of Metal-Organic Frameworks (MOFs) for Use in Liquid Chromatography
New developments in metal-organic frameworks (MOFs) for LC seek to control MOF distribution on the silica surface, improve understanding of the role of the substrate, and enhance robustness and reproducibility.
Perspectives and Pitfalls in Potency Testing of Cannabinoids by High Performance Liquid Chromatography (HPLC)
HPLC is emerging as the preferred analytical method for potency testing of cannabinoids, but fundamental work is needed to solve critical issues and contribute to advancing knowledge.
The 2022 Winners of the Lifetime Achievement and Emerging Leader in Chromatography Awards
Barry L. Karger and James P. Grinias are the winners of the 15th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively, for 2022. Here, we review their achievements.
A Quick and Accurate High Performance Liquid Chromatography (HPLC) Method to Determine the Amount of Trimethylamine in Fish Oil Softgels and Multivitamin Softgels Containing Fish Oil
A HPLC method was developed to measure trimethylamine in fish oil supplements, to determine if the level of this compound affects product quality attributes such as rancidity.
The LCGC Blog: Mentorship in the Time of COVID-19: Finding the Silver Lining and Announcing ACS SCSC Initiatives
An undeniable effect of the COVID-19 pandemic is that we have learned how to adapt (quickly) to sudden changes.
But Why Doesn’t It Get Better? Kinetic Plots for Liquid Chromatography, Part I: Basic Concepts
Kinetic plots can help us understand how different combinations of parameters will perform in relation the time needed to acquire a particular column efficiency—and thus resolution.
The QuEChERSER Mega-Method
QuEChERS has been updated to suit modern instrumentation. Now also “efficient and robust,” QuEChERSER is a “mega-method” that covers a wider polarity range.
Two-Dimensional Liquid Chromatography (2D-LC): Analysis of Size-Based Heterogeneities in Monoclonal Antibody–Based Biotherapeutic Products
We review different approaches and coupling strategies for analyzing monoclonal antibody aggregates with 2D-LC.
Exploring the Implementation of Compact Chromatographic Instrumentation in Common Analytical Workflows
Compact instrumentation offers important advantages for many workflows, as illustrated by these examples.
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