HPLC

LCGC and Spectroscopy Editor Patrick Lavery spoke with Oligo Factory CEO Chris Boggess about the company’s recently attained compliance with Good Manufacturing Practice (GMP) International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) Expert Working Group (Q7) guidance and its distinction from Research Use Only (RUO) and International Organization for Standardization (ISO) 13485 designations.

Scientists in Xi’an, China have created a novel system for the analysis of cell membranes, based around haloalkane dehalogenase protein tag (HALO)-tag technology. Their research was published in Talanta.

This experiment introduced a deep eutectic solvent developed in conjunction with a pH-induced phase transition combined with high performance liquid chromatography (HPLC) for efficient trace determination of bisphenol A (BPA) in water samples.

In this LCGC Blog, the author explores stationary phases that incorporate permanent or induced charges within, or alongside, hydrophobic alkyl silica type bonded ligands.

Scientists from Hunan University of Chinese Medicine and Zhengzhou University in Zhengzhou, China recently created a new system for extracting catecholamines (CAs) in urine samples.

The research conducted by Jing Dong and team unveils a pioneering approach that not only unravels the complexities of Valeriana jatamansi, but also showcases the potential of advanced analytical techniques in comprehensively studying natural compounds.

This research introduces a novel chromatographic method for the simultaneous determination of bisoprolol fumarate, carvedilol, and furosemide in both their pure and pharmaceutical dosage forms, offering a cost-effective, time-saving solution for routine drug quality control analysis.

In a recent study out of the University of Gdańsk, immobilized artificial membrane chromatography (IAM) was tested for its ability to evaluate small molecular behavior in the human body.

This paper proposes a new method of flash qualitative identification (FQI) to qualitatively identify a certain target component from a mixture within half a second by disusing the analytical column, which is a time-consuming unit in current chromatography instruments. First, a Noised Spectrum Identification (NSI) model was constructed for the data set generated directly by diode array detector (DAD) without the process in an analytical column. Then, a method called vector error algorithm (VEA) was proposed to generate an error according to the DAD data set for a mixture and a specific spectrum for the target component to be identified. A criterion based on the error generated by the VEA is used to give a judgement of whether the specific spectrum exists in the DAD data set. Several simulations demonstrate the high performance of the FQI method, and an experiment for three known materials was carried out to validate the effectiveness of this method. The results show that the NSI model concurs with the real experiment result; therefore, the error generated by the VEA was an effective criterion to identify a specific component qualitatively, and the FQI method could finish the identification task within half a second.

You're invited to publicize your 2023–2024 new product introductions in LCGC. Here's how to get your products included.

A group of scientists from Xi’an, China have created a new system for analyzing cell membranes based around HALO-tag technology.

A novel analytical method for the analysis of OLED materials using SFC is presented.

There are three important habits and practices that can improve the effectiveness and efficiency of any troubleshooter.

We explore advancements in silica particles for liquid chromatography, focusing on the shift toward improved monodispersity and its impact on chromatographic parameters, efficiency, backpressure, and sensitivity in high-performance liquid chromatography (HPLC).

The authors explore computer-aided workflows and machine learning, aimed at optimizing LC parameters, focusing on kinetic and thermodynamic aspects, and proposes closed-loop optimization strategies.

This article provides a high-level overview of Current Good Manufacturing Practice (cGMP) regulations and related public quality standards.

A novel study conducted at Nicolaus Copernicus University in Poland explores the potential of diatom-derived biosilica as a stationary phase in high-performance liquid chromatography (HPLC).

In a recent study conducted by two researchers from the CSIR-Indian Institute of Toxicology Research and the Academy of Scientific and Innovative Research, an analytical method was used to reveal coccidiostat residues in poultry.

Two scientists from Baskent University in Ankara, Turkey analyzed multiple proteins in cream formulation using reverse-phase high-performance liquid chromatography.

For the first time, inhibitors of a cyclooxygenase isoform were found in Allium ampeloprasum L., specifically the giant or “elephant” garlic grown in the Chiloé Archipelago.

At EAS 2023, Michael Dong and Szabolcs Fekete reviewed certain innovations in high performance liquid chromatography (HPLC), and in what ways scientists can continue to improve the quality of their experiments.

Scientists from Egypt have developed a green reversed-phase high-performance liquid chromatography (RP-HPLC) method to determine the amounts of methionine and paracetamol in different mixtures.

An efficient solvent system screening approach that streamlines fractionation of compounds with similar UV absorption eliminates the need for individual partition coefficient calculations, and coupled with preparative HPLC purification, led to the isolation of seven c-glycosyl flavonoids and an amino acid from barley seedlings.

Scientists from Ames National Laboratory in Ames, Iowa recently analyzed silver(I) and copper(I) ions to understand the environmental factors when they are altered.

We provide a high-level overview of Current Good Manufacturing Practice (cGMP) regulations and their impact on the analytical chemist in the pharmaceutical industry.

A team of scientists from Nanjing, China, have created a low-cost efficient online derivatization high-performance liquid chromatography (HPLC)-ultraviolet (UV) detector system, which they used to detect mono- and oligo-saccharides.

This month we interview Zhuoheng Zhou from the Vrije Universiteit Brussel in Belgium, about his focus on advancing instrumental and column technologies for modern chromatography and its applications in novel biopharmaceutical modalities and proteomics. We also spoke to him about his work developing a protocol to help with the design of polymer monolithic capillary columns.

In a recent study, a novel chiral column, based on a CC19-R porous organic cage, promises to enhance enantioselective HPLC separations.

Researchers have developed a meticulous method for analyzing impurities in a product used for early treatment of breast cancer in premenopausal and perimenopausal women, shedding light on the product’s degradation pathways.

A comparison between two types of detectors—variable wavelength (VWD) and fluorescence (FLD)—in quantifying azoxystrobin from water samples was conducted.