Multidimensional LC

The 18th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology will take place from 28–31 May 2024 in Leuven, Belgium.

This month we interview Shijia Tang, principal scientist at Genentech, South San Francisco, CA, USA, about her innovative research focusing on the analysis of polymers and nanomaterials used in pharmaceutical analysis. Shijia decribes the benefits of an innovative in-line mixing modulation method for 2D-LC she developed for polymer and oligonucleotide analysis. The advantages of SEC–MALS to explore aggregation mechanisms for therapeutic constrained peptides and mRNA-lipid nanoparticles and their aggregates are also discussed.

This review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.

The 15th Multidimensional Chromatography Workshop is a free event involving keynote and contributed presentations, a poster session, and discussion groups on all multidimensional techniques, and will be held in-person on the campus of California State University, Los Angeles, from January 10 to 12, 2024.

A comprehensive analytical approach utilizing online two-dimensional liquid chromatography coupled with hybrid linear ion trap orbital trap mass spectrometry, has enabled the in-depth analysis of Huangqi Guizhi Wuwu decoction, identifying 170 chemical constituents and offering insights into its complex chemical composition and potential therapeutic properties.

At HPLC 2023, John Langley, a professor at the University of Southampton in Southampton, UK, presented a talk called "(R)evolution of Hyphenations, Through Open-Access RP-HPLC–MS, Open-Access UHPSFC–MS and 2D-LC–IMS-MS in Academia". He spoke with LCGC on the importance of open-access hyphenation, and how to implement it in your research.

The methodologies demonstrated here open the way to fully compliant at-line monitoring of monoclonal antibody quality attributes.

Researchers from Corteva Agriscience tested a new approach for separating and quantifying small molecule structure elucidation.

The 27th International Symposium on Separation Sciences (ISSS 2023) will be held 24–27 September 2023 in Cluj-Napoca, Romania. Here’s a sneak preview of what attendees can look forwards to.

This instalment of “Column Watch” presents many of the highlighted topics and trends observed at this exceptionally well-run symposium.

Analyzing representative standard mixtures, APIs, and synthetic impurities shows that when TRLC is combined with RPLC in 2D-LC, separation performance is improved. We explain why.

Increases and decreases in anthocyanin concentration, which relates to the level of sugars found in red wine, were observed at specific weekly intervals of the grape ripening process.

On a fundamental level, equilibrium and diffusion define chemical separations.

High-throughput proteomic research has been hampered by the limited peak capacity of one-dimensional liquid chromatography (1D-LC) separation performance.

The two-dimensional liquid chromatography (2D-LC) separations were supplemented by the use of capillary-channeled polymer (C-CP) fiber columns.

Researchers have developed a trapping mode two-dimensional liquid chromatography (2D-LC) workflow for the enrichment and quantification of low-level impurities in pharmaceutical development, offering a promising approach for enhancing impurity monitoring in the pharmaceutical industry.

Authentication of popular ginseng-based products has been difficult and has caused confusion in quality control between traditional Chinese medicine (TCM) and Chinese patent medicine (CPM).

At HPLC 2023, LCGC spoke with Andrea Gargano of the Van 't Hoff Institute for Molecular Science at the University of Amsterdam in The Netherlands. These clips comprise one of several HPLC interviews that were conducted at the conference.

At HPLC 2023, Paola Dugo held a lecture on how combining two-dimensional liquid chromatography (LC×LC) with mass spectrometry (MS) can help scientists analyze food samples.

Talks by Dwight R. Stoll, Tadeusz Górecki, André de Villiers, and Andrea Gargano highlighted the after-lunch presentations at HPLC 2023 on June 20.

Peter Schoenmakers of the University of Amsterdam presented a talk at HPLC 2023 on multidimensional liquid chromatography (LC).

Scientists have developed a breakthrough MDLC–MS method for in-depth characterization of charge variants in therapeutic antibodies. The innovative approach enables the detection and evaluation of rare charge variants, facilitating improved safety and efficacy in antibody development.

Organic acids, flavonoids, iridoids, and saponins were among the compounds discovered, with significant α-glucosidase inhibitory behavior in vitro that was inconsistent with substance content level.

Researchers have developed an innovative strategy for compound annotation in offline 2D-LC–MS analysis. The approach combines hand-in-hand alignment with targeted molecular networking (TMN) and was successfully applied to analyze the chemical profile of Yupingfeng (YPF), a traditional Chinese medicine prescription, resulting in the identification of 497 compounds and demonstrating its efficiency and scalability in complex sample analysis.

This study suggests a 2D-LC screening method to evaluate peak purity and ensure specificity in pharmaceutical analysis, thereby promoting the safe production of medicines by detecting active pharmaceutical ingredients (API) and related substances.