April 12th 2024
Here are the top five articles that the editors of LCGC International published this week.
September 13th 2023
Carotenoids are a class of natural pigments, widely distributed in vegetables and fruits. A comprehensive two-dimensional liquid chromatography (LC×LC) method, based on the use of a cyano and an octodecylsilica column, placed in the first and second dimension, respectively, was applied to evaluate carotenoid composition and stability in selected overripe fruits representing the waste generated by a local food market. This research also evaluates if post-climacteric biochemical changes are linked to carotenoid degradation in the investigated fruits. A total of 22 compounds was separated into seven different chemical classes in the two-dimensional space, and identified by photodiode array (PDA) and mass spectrometry (MS) detection. The results prove that the waste generated by the large distribution of food still represent an important source of bioactives that could be used for other purposes.
Carotenoids are a class of natural pigments, widely distributed in vegetables and fruits. A comprehensive LC×LC method, based on the use of a cyano and an octodecylsilica column, placed in the first and second dimension, respectively, was applied to evaluate carotenoid composition and stability in selected overripe fruits representing the waste generated by a local food market.
The Modulator in Comprehensive Two-Dimensional Liquid Chromatography
May 20th 2016The interface between the two separation dimensions is a key element of any comprehensive two‑dimensional liquid chromatography (LC×LC) system. LC×LC has typically been implemented by using one or more switching valves, equipped with either sampling loops or trap column(s). Temperature manipulation is a relatively unexplored yet promising route towards non-valve-based LC×LC. The fairly recent emergence of thermal modulation has provided a less conventional method for performing LC×LC separations. This article illustrates the variety of commonly used modulators, paying specific attention to focusing modulators.
Quantification of Purine Alkaloids and Catechins in Green and Black Tea Using Comprehensive 2D-LC
December 1st 2015This application note discusses the comprehensive 2D‑LC analysis of green and black tea. The purine alkaloids caffeine and theobromine, as well as the catechins catechin, epicatechin, and epigallocatechin gallate, are quantified.
Quantification of Purine Alkaloids and Catechins in Green and Black Tea Using Comprehensive 2D-LC
December 1st 2015Comprehensive 2D-LC enables the analysis and quantification of purine alkaloids and catechins in green and black tea. As expected, green tea contained higher amounts of the catechins epigallocatechin gallate and epicatechin than black tea.
Trace Analysis of Estrogen Using 2D LC–MS–MS
May 7th 2015Estrogen can have a big effect on physiological processes in the human body, even when present at trace levels. In this video, Kevin Schug from the University of Texas at Arlington, USA, talks about the advantages of using restricted access media for the on-line sample preparation of biofluids in a “trap-and-elute” LC–MS arrangement, and the potential of this approach for other applications.
Determination of Phenolic Compounds in Virgin Olive Oil Using Comprehensive 2D-LC
March 1st 2015This application note demonstrates how comprehensive 2D-LC can be used to resolve the complex mixture of hydrophilic phenols found in virgin olive oil and investigates differences in the phenol composition of several olive oils.
Determination of Phenolic Compounds in Virgin Olive Oil Using Comprehensive 2D-LC
June 1st 2014This application note demonstrates how comprehensive 2D-LC can be used to resolve the complex mixture of hydrophilic phenols found in virgin olive oil and investigates differences in the phenol composition of several olive oils.
Researchers Compare Configurations for Two-Dimensional LC for the Analysis of Peptides
August 21st 2013In new research, separation scientists from the University of Lyon, France, have compared different configurations of on-line two-dimensional liquid chromatography (2D LC) for the separation of charged compounds such as peptides.