In this edition of The LCGC Blog, Jonathan Shackman discusses what factors to consider when choosing a mobile phase for liquid chromatography (LC) procedures.

High performance liquid chromatography (HPLC) is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture | Image Credit: © Love Employee - stock.adobe.com

Selecting Optimal Two-Dimensional Liquid Chromatography Columns

By Aaron Acevedo

January 30th 2025

University College London scientists recently created a new technique for selecting two-dimensional liquid chromatography (2D-LC) columns.

Enzyme structure | Image Credit: © Artur - stock.adobe.com

Detailed Glycosylation Analysis of Therapeutic Enzymes Utilizing Comprehensive 2D-LC–MS

By Koen Sandra
Kathleen Piens

Debby Bracke
Pat Sandra
Wouter Vervecken

January 3rd 2025

In this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.

Caitlin N. Cain is a postdoctoral research fellow at the University of Michigan. She is supported by the National Institutes of Health – National Research Service Award for Individual Postdoctoral Fellows. Her research specializes in the development of chemometric techniques to improve chromatographic non-targeted analyses. Previously, she earned her Ph.D. in Chemistry from the University of Washington in 2024 and B.S. degrees in Chemistry and Forensic Science from Virginia Commonwealth University in 2019. Her research efforts have been recognized by numerous accolades, including the inaugural LCGC Rising Stars of Separation Science Award. She proudly serves as an executive committee member for the ACS SCSC (Subdivision on Chromatography and Separations Chemistry).

The LCGC Blog: Reunifying Separation Science

By Caitlin N. Cain

January 3rd 2025

In the first LCGC Blog of 2025, Caitlin Cain of the University of Michigan highlights the divide between gas and liquid chromatographers, and why both types of techniques should be in the same conversations.

Thin layer chromatography equipments include jar, silica gel, capillary and compounds. TLC method used in purity analysis of compounds in chemistry laboratory. | Image Credit: © mehmet - stock.adobe.com

Next Generation Peak Fitting for Separations

By M. Farooq Wahab
Troy T. Handlovic

Daniel W. Armstrong

December 11th 2024

Separation scientists frequently encounter critical pairs that are difficult to separate in a complex mixture. To save time and expensive solvents, an effective alternative to conventional screening protocols or mathematical peak width reduction is called iterative curve fitting.

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