Application Notes: Food and Beverage

An isocratic HPLC method showing the separation of an artificial sweetener in a cola beverage. The chromatogram shows the advantage of using 230nm instead of 254nm for improved detection but minimizing the baseline interferences.

195 pesticides were chosen to evaluate both the traditional time segment (TS) acquisition and dMRM acquisition structures. The use of dMRM provides the capability to better tackle large multi-analyte analysis.

A variety of alcoholic beverages purchased within the UK were analyzed for the presence of Ethylcarbamate. A 1 µL injection of these alcoholic beverages were analyzed on an Ellutia 200 GC followed by detection on an Ellutia 820 TEA working in nitrogen detection mode. This mode of analysis detects nitrogen containing compounds within a sample. The Ellutia 810/820 systems are able to analyse various nitro or nitroso compounds, utilizing the selectivity available through nitrogen or nitroso modes. Nitrogen mode utilizes the catalytic pyrolyzer tube, oxygen reactor, and higher temperatures to detect nitrogen compounds. Results for various alcoholic beverages are shown in Figure 3. The samples were found to have various low levels of ethylcarbamate, the system was optimized through levels of oxygen input and EHT sensitivity. The samples were then analyzed adopting a standard addition approach.

The standards are commercially available from Wellington Laboratories Inc. (Ontario, Canada): TF-TCDD-MXB.

Analysis of the six matrices processed yielded acceptable recoveries for all analytes with standard deviations below 20%. Analysis of an n-hexane blank sample resulted in no detectable target analytes measured within the calibration range of each respective compound.

Commission Regulation (EC) No 152-2009 published in the official Journal of European Union laid down the methods of sampling and analysis for the official control of feed. The Regulation describes methods of analysis to control the composition of feed materials and compound feed products. Establishing the amino acids profile is an important way to control quality and nutritional value of feeds.

Comprehensive 2D-LC enables the analysis and quantification of purine alkaloids and catechins in green and black tea. As expected, green tea contained higher amounts of the catechins epigallocatechin gallate and epicatechin than black tea.

Homogenize 2 g of dry tea leaves with 25 mL of Li220 for 5 min. Centrifuge and filter through 0.45 µm nylon filter.

Following sampling, tubes were transferred to a Markes UNITY™ thermal desorption system paired with an Agilent 7890B GC that was coupled to an Agilent 5977A MS detector. The UNITY™ system and GC–MS parameters are presented in Table I and Table II, respectively.

Theanine is a neurologically active amino acid found in tea plants. L-Theanine is a dominant amino acid in green tea and is responsible for its unique pleasant taste as well as known relaxation effect.

Mycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply