Application Notes: General

Chemical characterization of medical devices, including drug-device combination products, is crucial for ensuring regulatory compliance and patient safety by identifying and quantifying chemicals that may interact with the human body. This paper explores current trends, best practices, and regulatory developments in extractables and leachables (E&L) testing for medical devices.

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

Garlic has been found to exhibit a multitude of proliferative health benefits in the realm of cardiac events.

A reversed-phase HPLC method was developed to separate five steroid hormones, with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone).

Sulfide compounds found in garlic oils are used in therapeutics and prophylaxis of certain cancers, and are prevalent in cell health.

Identification of the five most common saccharides found in packaged foods, lactose, maltose, sucrose, fructose, and glucose, is easily resolved on the RCX-30 column.

The Raffinose Family of Oligosaccharides (RFO) is comprised of low molecular weight non-reducing sugars. Analysis of these compounds is not easily done unless using HC-75.

Isolation of six common allergens can be achieved by utilizing reversed-phase HPLC using an alkaline eluent.

With the growing utility of the K vitamin series of molecules, a need appears relevant for both food labeling of constituents and for research based separation of the natural and synthetic compounds of vitamin K. The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple efficient identification of the whole vitamin series (K2, K3, and K4).

SEC data demonstrates mRNA aggregate analysis for vaccine stability. Optimal separation occurs at 100 mM NaCl and low flow rates, enhancing mRNA production quality.

The data show that a separation between circular and linear plasmid DNA is possible and the performed linearization can be monitored using SEC-HPLC.

This application showcases the utility of SEC to resolve s.s. oligonucleotides from duplexes in mixtures of four oligonucleotide lengths (21, 40, 80, and 100mers).

Experience high recoveries from the first injection! YMC Accura BioPro IEX SF columns provide excellent peak shapes for the ion-exchange analysis of mAbs.

The optimized LC-MS/MS method covering 388 signaling lipids shows that the bioinert coated YMC Accura Triart C18 column combines high sensitivity with high resolution.

In this application note, the charge variant characterisation of IgG4-based mAbs was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.

The non-targeted screening of polar metabolites in human plasma with high sensitivity is achieved using a bioinert YMC Accura Triart Diol HILIC column.

The analysis of human plasma samples using a bioinert coated YMC Accura Triart C8 column demonstrates that real-life lipidomic analysis in under 10 minutes is possible.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.

This application note demonstrates the analysis of 9 halogenated acetic acids, which are regulated by the EPA and the European Union, using ICPMS/MS for detection.

The Axcend Focus LC®, a portable capillary liquid chromatograph, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for analysis of pharmaceutical drugs.

Compact HPLC Specification

Axcend’s revolutionary line-up of eco-friendly, compact and portable HPLC instruments and accessories are engineered to deliver consistent, high-quality performance, ensuring precise and dependable analytical results. Compact and portable, the Axcend Focus LC deliver HPLC Anywhere™.

A demonstration of emptying a waste container of a compact and portable LC system.

The Axcend Focus LC® stands out as an eco-friendly high-performance liquid chromatograph (HPLC) due to its innovative design and sustainable features. This compact system uses significantly less solvent, consuming under 10 mL per week, and produces roughly the same amount of waste. Additionally, its energy consumption is a fraction of that of larger, bench-top LCs. By minimizing solvent use and waste production, the Axcend Focus LC not only reduces operational costs but also aligns with green analytical chemistry principles, making it an environmentally responsible choice for laboratories

Hear how Eurofins SOFIA GmbH successfully implemented the TSQ 9610 GC-MS/MS into their workflow for trac contaminant analysis and increased higher sample throughput using the combination of instrumentations and software.