Oligonucleotides—therapeutic agents for difficult-to-treat diseases—rely on precise molecular weight confirmation to ensure quality during development. Experts from Agilent Technologies explore the challenges of liquid chromatography-mass spectrometry (LC/MS) analysis in oligonucleotide synthesis, as well as strategies for accurate deconvolution, method development, and optimal equipment selection.
This application note examines the selectivity differences between three L1 columns. The CORTECS Premier C18, CORTECS Premier C18+ and CORTECS Premier T3 Columns were used to separated paracetamol and its impurities using EP monograph conditions.
In this application note, a USP monograph is modernized to a shorter column using 5 µm particles, which can be allowable under USP <621> as long as the N value is within the guidelines. By using highly efficient CORTECS 5 μm particles, this type of modernization is possible and reductions in solvent usage and run times are achieved.
This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.
In this work the development of two key attributes of the 5um CORTECS Columns is examined. First column efficiency is compared across CORTECS and other solid-core 5 μm columns. Next scalability from sub-2 μm to 5 μm particles is examined between the CORTECS Column lines and competitive column lines. It was found that CORTECS columns have higher efficiency compared to other solid-core columns and that CORTECS particles are fully scalable.
Trace-level analysis of VOCs in a tomato product using headspace extraction with large volume preconcentration (LVP) and multi-step enrichment (MSE)
This study demonstrates the Centri® sample extraction and enrichment platform’s unique multi-step enrichment feature to enhance the discovery of trace-level, packaging-derived contaminants in food and beverage samples by GC–MS.
Efficient separation N-glycans and isomers by HPAEC and quantification with Pulsed Amperometric Detection (PAD) and MS for identification.
This study demonstrates highly sensitive detection of residual fumigants in seeds and spices using MSE–HS–trap with GC–MS, achieving detection limits as low as 0.0018 mg/kg, well below EU regulatory limits. Excellent linearity (R² > 0.99), reproducibility, and throughput (40 samples/day) are achieved. Enhanced sensitivity with single-ion-monitoring (SIM) is also demonstrated for ethylene oxide and 2-chloroethanol.
Various samples, ranging from short-chain FOS to long-chain inulin including heterogenous blends of inulin-type fructans, can be easily analyzed using HPAEC-PAD.
Separation of oligo- and polysaccharides in honey up to DP40 within 25 minutes run time, enabling fast profiling of fraudulent/adulterated honey.
Optimized method for oligo– and polysaccharides profiling by separating fructo– and maltooligosaccharides for honey adulterations (fraud) measurements.
Fast separation (within 8 min) and quantification of lactose and its isomers in commercial lactose-free products using HPAEC-PAD.
Comprehensive analysis of fermentable an non-fermentable sugars in beer using the ALEXYS™ Carbohydrate Analyzer equipped with SweetSep™ AEX200 column.
Fast and sensitive method for the quantification of sucralose within 8 min in beverages and chewing gum using HPAEC-PAD with SweetSep™ column.
Application note outlining the methodology for assessing significant levels of PFAS emissions from food contact materials under typical usage conditions. PFAS were detected across a range of temperatures for the items tested, with concentrations varying from 1 pg/g to over 6000 pg/g.
This study shows how GC–MS performance for the sampling of aroma compounds and off-odours in beverages can be enhanced by using techniques incorporating trap-based preconcentration. The first part of the study focuses on SPME, and how trapping and enrichment can improve peak symmetry, qualitative analysis and sensitivity. The second part of the study compares these methods against automated robe-based high-capacity sorptive extraction, which as well as being operationally robust, ffers improved recovery for higher-boiling compounds and an extended analyte range.
High-resolution separation of maltodextrin with outstanding linearity, repeatability, and sensitivity, enabling the precise quantification of its components.
LPGC-MS Multiresidue Pesticides Analysis
An extraction method with an optional fluorenylmethyloxycarbonyl chloride derivatization step to allow for the analysis of glyphosate on a C18 HPLC column.
Evaporation enhances method sensitivity. This study investigates the impact of gas temperature and flow rate on blowdown evaporation of solvents and their mixtures
In collaboration with the organizers of the Recent Advances in Food Analysis 2024 conference in Prague, Czech Republic, LCGC International has created a comprehensive e-book featuring cutting-edge topics in food analysis. This collection includes articles on the latest analytical and bioanalytical methods for ensuring food quality and safety, along with interviews with this year’s presenters. Readers will gain valuable insights into current trends, challenges, and innovative solutions in food and natural products analysis.
Switching the GPC/SEC solvent from THF to 2-methyl-THF provides an easy swap to provide a greener, safer, and less toxic option.
Inside the Laboratory is a joint series with LCGC International and Spectroscopy, profiling analytical scientists and their research groups at universities all over the world. This series spotlights the current chromatographic and spectroscopic research their group is conducting, and the importance of their research in analytical chemistry and specific industries.
Top-down fragmentation enables rapid characterization of phosphorylated proteins without extensive sample preparation and digestion. In this study, electron capture dissociation (ECD) was used to fragment proteoforms of the cell death-related protein, Bcl-xL. Using these methods, 85–90% sequence coverage was achieved for Bcl-xL proteoforms, allowing for effective localization of phosphorylation within minutes.
Enhanced antibody analysis using electron capture dissociation (ECD) allows for precise glycan localization in low-abundance glycopeptides. This study compares the fragmentation of trastuzumab tryptic digests using ECD and collision-induced dissociation (CID). While CID generates abundant glycan HexNAc ions at 204 m/z, ECD preserves the labile glycan group, enabling accurate site localization.
Volatile flavor and aroma compounds are extracted and determined in e-liquids for regulatory compliance purposes using SBSE-TD-GC-MS, in an efficient solvent-free workflow.
Electron capture dissociation produces distinct fragments of amino acid side chains, enabling the identification of isomeric amino acids such as leucine and isoleucine. This application note demonstrates the isomer identification workflow for peptides and intact proteins using the new Agilent ExD cell and ExDViewer software for fragment analysis.