Application Notes: General

An extraction method with an optional fluorenylmethyloxycarbonyl chloride derivatization step to allow for the analysis of glyphosate on a C18 HPLC column.

Evaporation enhances method sensitivity. This study investigates the impact of gas temperature and flow rate on blowdown evaporation of solvents and their mixtures

In collaboration with the organizers of the Recent Advances in Food Analysis 2024 conference in Prague, Czech Republic, LCGC International has created a comprehensive e-book featuring cutting-edge topics in food analysis. This collection includes articles on the latest analytical and bioanalytical methods for ensuring food quality and safety, along with interviews with this year’s presenters. Readers will gain valuable insights into current trends, challenges, and innovative solutions in food and natural products analysis.

Switching the GPC/SEC solvent from THF to 2-methyl-THF provides an easy swap to provide a greener, safer, and less toxic option.

Inside the Laboratory is a joint series with LCGC International and Spectroscopy, profiling analytical scientists and their research groups at universities all over the world. This series spotlights the current chromatographic and spectroscopic research their group is conducting, and the importance of their research in analytical chemistry and specific industries.

Top-down fragmentation enables rapid characterization of phosphorylated proteins without extensive sample preparation and digestion. In this study, electron capture dissociation (ECD) was used to fragment proteoforms of the cell death-related protein, Bcl-xL. Using these methods, 85–90% sequence coverage was achieved for Bcl-xL proteoforms, allowing for effective localization of phosphorylation within minutes.

Enhanced antibody analysis using electron capture dissociation (ECD) allows for precise glycan localization in low-abundance glycopeptides. This study compares the fragmentation of trastuzumab tryptic digests using ECD and collision-induced dissociation (CID). While CID generates abundant glycan HexNAc ions at 204 m/z, ECD preserves the labile glycan group, enabling accurate site localization.

Electron capture dissociation produces distinct fragments of amino acid side chains, enabling the identification of isomeric amino acids such as leucine and isoleucine. This application note demonstrates the isomer identification workflow for peptides and intact proteins using the new Agilent ExD cell and ExDViewer software for fragment analysis.

This application note provides an overview of JEOL’s innovative msFineAnalysis AI and its capabilities for enhancing GC-MS analysis.

Chemical characterization of medical devices, including drug-device combination products, is crucial for ensuring regulatory compliance and patient safety by identifying and quantifying chemicals that may interact with the human body. This paper explores current trends, best practices, and regulatory developments in extractables and leachables (E&L) testing for medical devices.

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

Garlic has been found to exhibit a multitude of proliferative health benefits in the realm of cardiac events.

A reversed-phase HPLC method was developed to separate five steroid hormones, with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone).

Sulfide compounds found in garlic oils are used in therapeutics and prophylaxis of certain cancers, and are prevalent in cell health.

Identification of the five most common saccharides found in packaged foods, lactose, maltose, sucrose, fructose, and glucose, is easily resolved on the RCX-30 column.

The Raffinose Family of Oligosaccharides (RFO) is comprised of low molecular weight non-reducing sugars. Analysis of these compounds is not easily done unless using HC-75.

Isolation of six common allergens can be achieved by utilizing reversed-phase HPLC using an alkaline eluent.

With the growing utility of the K vitamin series of molecules, a need appears relevant for both food labeling of constituents and for research based separation of the natural and synthetic compounds of vitamin K. The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple efficient identification of the whole vitamin series (K2, K3, and K4).

This technical note demonstrates the SEC-based separation and quantification of Cas9 IVT mRNA (with a total length of approximately 4500 nucleotides) and its aggregates.

SEC data demonstrates mRNA aggregate analysis for vaccine stability. Optimal separation occurs at 100 mM NaCl and low flow rates, enhancing mRNA production quality.

The data show that a separation between circular and linear plasmid DNA is possible and the performed linearization can be monitored using SEC-HPLC.

This application showcases the utility of SEC to resolve s.s. oligonucleotides from duplexes in mixtures of four oligonucleotide lengths (21, 40, 80, and 100mers).

Experience high recoveries from the first injection! YMC Accura BioPro IEX SF columns provide excellent peak shapes for the ion-exchange analysis of mAbs.

The optimized LC-MS/MS method covering 388 signaling lipids shows that the bioinert coated YMC Accura Triart C18 column combines high sensitivity with high resolution.

In this application note, the charge variant characterisation of IgG4-based mAbs was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.

The non-targeted screening of polar metabolites in human plasma with high sensitivity is achieved using a bioinert YMC Accura Triart Diol HILIC column.