Application Notes: General

Fully automated method for 3-MCPD, 2-MCPD and Glycidol determination in Edible Oil and Fat based on ISO 18363-4 - Zwagerman/Overman with validation data. A recent upgrade to PTV injection has further improved the quality and robustness of results. Fatty acid esters of 3- and 2-monochloropropanediol (3-MCPD-e, 2-MCPD-e) and glycidol (Gly-e) are process contaminants that are formed, for example, when edible oils and fats are refined. After ester cleavage during digestion in the human body they pose a relevant health risk and therefore need to be determined in edible oils and fats and in fat containing food.

The AppNote describes the fully automated determination of MOSH/MOAH in Food and Packaging extracts following DIN EN 16995. Industrial production, processing and transportation invariably put food at risk of contamination with MOSH/MOAH. To ensure a reasonable cost benefit balance, high laboratory productivity and good quality of results, leading contract laboratories increasingly strive to automate their processes. An example is the determination of MOSH/MOAH using a LC-GC-FID Coupling Platform. Depending on the sample matrix, additional automated sample preparation by aluminum oxide clean-up, epoxidation, and/or saponification is necessary prior to analysis. The dedicated evaluation software integrates the complex MOSH/MOAH chromatograms accurately and reproducibly.

When it comes to water analysis, it can be challenging for labs to keep up with ever-changing testing regulations while also executing time-efficient, accurate, and risk-mitigating workflows. To ensure the safety of our water, there are a host of national and international regulators such as the US Environmental Protection Agency (EPA), World Health Organization (WHO), and the European Union (EU) that demand stringent testing methods for drinking water and wastewater. Those methods often call for fast implementation and lengthy processes, as well as high sensitivity and reliable instrumentation. This paper explains how your ICP-MS, ICP-OES, and LC-MS-MS workflows can be optimized for compliance with the latest requirements for water testing set by regulations like US EPA methods 200.8, 6010, 6020, and 537.1, along with ISO 17294-2. It will discuss the challenges faced by regulatory labs to meet requirements and present field-proven tips and tricks for simplified implementation and maximized uptime.

Hamilton has produced a series of reference guides to assist science professionals in finding the best-suited products and configurations for their applications. The Syringe and Needle Reference Guide provides detailed information on Hamilton Company’s full portfolio of syringes and needles. Everything from cleaning and preventative maintenance to individual part numbers are available for review. It also includes selection charts to help you choose between syringe terminations like cemented needles and luer tips.

Magic mushrooms, once taboo, have recently experienced a renaissance. This new awakening is partially due to new findings that indicate the effects of psilocybin, and its dephosphorylated cousin psilocin may produce long lasting results for patients who might be struggling with anxiety, depression, alcohol and drug abuse, and post-traumatic stress disorder. Hamilton Company has developed a methodology for the isolation and identification of 5 common psychedelic compounds used in the potential treatment of disease. The PRP-1 HPLC column resin remains stable in the harsh alkaline conditions ideal for better separations.

Top quality materials and skilled workmanship ensure Hamilton syringes consistently deliver the highest possible performance for reliable analyses. With proper care and handling, Hamilton syringes provide unsurpassed performance year after year. This guide covers daily syringe use along with safety information and troubleshooting tips.

The G protein-coupled receptors (GPCR) are found throughout the entire body and have shown significance in the development of new therapeutic treatments. Isolation of seven classic GRPC peptides initiating in the GI tract highlights the benefits of using the polymer-based PRP-3, a reversed-phase resin. The covalent bonds found in the PRP-3 exhibit advantageous interactions between the biological π bonds found in the peptides and the available aromatic benzyl rings of the resin.

This application note demonstrates the superior resolution and recovery of Agilent AdvanceBio SEC 500 Å columns when compared with competitor products for AAVs.

This app note describes a non ion-pairing reversed-phase LC/MS method for highly modified ASOs, DNA, sgRNA, and siRNA ranging from 14- to 103-mer oligonucleotides.

This application note demonstrates a liquid chromatography/quadrupole time-of-flight mass spectrometer (LC/Q-TOF MS) workflow for the identification and characterization of therapeutic peptides.

An optimized liquid chromatography (LC) method offers improved impurity separation, no batch reproducibility issues, and is unaffected by changes in gradient delay volume settings.

Discover how Chong Kun Dang (CKD), a leading South Korea Pharmaceutical Company, has transformed the operation of chromatography systems in their Quality Control (QC) Lab with the implementation of waters_connect System Monitoring.

Frontage laboratories wanted to improve the efficiency and quality of their operations and invest in ways to streamline their workflows. They implemented Waters NuGenesis™ Lab Management System (LMS) to progress their digital transformation journey and combine synergistic data, workflow, and sample management capabilities to support the entire product lifecycle from discovery through manufacturing.

Discover the principles, technologies, and myriad of benefits arising from the interconnected nature of networked LC-MS systems within analytical laboratories. Learn how LC-MS systems can empower you in your lab to excel in optimizing data generation and enhance overall efficiency.

Shandong Loncom Pharmaceutical Co. implemented NuGenesis LMS to help with generation of reliable and compliant production data, address electronic data management challenges, achieve accurate and efficient sample tracking, and manage increasing amounts of data. Learn more about how NuGenesis™ LMS enables labs to collect, record, and analyze experimental data for improved operational efficiency and data accuracy.

This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.

Now there's an single-purpose HPLC with a simplified UI that makes it possible for anyone to perform HPLC testing with a sinple one-button pass/fail test function.

Welcome to our comprehensive selection of High-Performance Liquid Chromatography (HPLC) cartridges. Designed for precision and reliability, our cartridges cater to diverse analytical needs, ensuring optimal performance in your laboratory processes.

The solution is an automated reaction monitoring system capable of unattended sampling, identification, and quantification of reaction components via HPLC. Axcend system is the only separations Process Analytical Technology (PAT) system that’s small enough to be placed in a hood or immediately adjacent to the reaction vessel.

Application Notebook highlighting Liquid Chromatography techniques commonly used for monitoring Critical Quality Attributes (CQAs) in Bioprocessing Applications

This application note demonstrates how THF mobile phases can be replaced to implement greener, safer, and less toxic SEC methods for polymer characterization.

This application note highlights the benefits of the LipiFiltr® cartridge for multi-class, multi-residue pesticide analysis in complex, high-fat samples.

Agilent and Scitara are collaborating not only to increase the efficiency of laboratory processes, but to enable customers to leverage the value of the data for various stakeholders. This paper will present an ecosystem designed to support enterprises in accelerating data workflows and improving data availability for researchers, lab analysts, and data scientists.

This study demonstrates high-throughput analysis of BTEX compounds from several matrices (air, water and soil). Detection limits in the single-digit part-per-billion concentration range (by volume) are readily achievable within seconds using SIFT-MS because sample analysis is achieved without chromatography, pre-concentration, or drying. We also present a calibration approach that enables speciation of ethylbenzene from the xylenes in real-time.