The Application Notebook-02-01-2019

Separation of Five Steroids on a Hamilton PRP-C18 Reversed-Phase HPLC Column

February 01, 2019

Medical/Biological

In this study, a reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.

Characterization of TSKgel FcR-IIIA-NPR HPLC Column by Top Down Mass Spectrometry

February 01, 2019

Medical/Biological

The purpose of this study is to demonstrate the use of mass spectrometry to characterize the elution profile of a typical IgG1 molecule separation on a TSKgel FcR-IIIA-NPR column, and verify the observations that certain glycan structures impart higher activity to the monoclonal antibody, especially as it relates to the presence of terminal galactose sugars.