The Application Notebook-02-01-2019
Automated SPE to Improve the Recovery of Semivolatile Compounds in Compliance with EPA Method 8270
February 01, 2019
Environmental
This applicate note presents the use of an automated solid-phase extraction (SPE) system to improve the accuracy and precision of semivolatile extractions, in compliance with EPA Method 8270.
Why are C18s So Different? A Focus on the Contributions of Steric Selectivity
February 01, 2019
General
One of the fundamental tenets of chromatography is the interaction between an analyte and stationary phase.
Separation of Five Steroids on a Hamilton PRP-C18 Reversed-Phase HPLC Column
February 01, 2019
Medical/Biological
In this study, a reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.
Restek LC–MS/MS Analysis of Mycotoxins in Peanut Powder in 5.5 Min
February 01, 2019
Food & Beverage
This method showed excellent precision and accuracy for the 12 FDA regulated mycotoxins that were evaluated during a validation study that covered a variety of matrices.
An Analysis of Amino Acids in Oxidized and Unoxidized Feed Samples
February 01, 2019
Food & Beverage
Pickering Laboratories developed an analytical method to comply with all the chromatographic requirements of Commission Regulation (EC) No 152-2009.
Add More Confidence to Your UHPLC–MS Analyses
February 01, 2019
Pharmaceutical/Drug Discovery
For your highly sensitive UHPLC–MS analyses, how can you reduce noise and additional signals to a minimum?
An Absolute Molar Mass Analysis of Chitosans
February 01, 2019
Medical/Biological
Chitosans are analyzed by SEC-MALS to determine molar mass moments and distributions.
Thermal Extraction of Phthalates in Polymers Based on IEC Method 62321-8 Using a Pyroprobe
February 01, 2019
Polymer
This application note presents calibration plots, RSDs, and MDLs for IEC Method 62321-8 using a CDS Model 6150.
Characterization of TSKgel FcR-IIIA-NPR HPLC Column by Top Down Mass Spectrometry
February 01, 2019
Medical/Biological
The purpose of this study is to demonstrate the use of mass spectrometry to characterize the elution profile of a typical IgG1 molecule separation on a TSKgel FcR-IIIA-NPR column, and verify the observations that certain glycan structures impart higher activity to the monoclonal antibody, especially as it relates to the presence of terminal galactose sugars.
An Extraction of Loperamide and N-Desmethyl Loperamide from Blood Followed by LC–MS/MS Analysis
February 01, 2019
Medical/Biological
This application note effectively outlines a simple three-step method for the extraction of loperamide and its main metabolite, N-desmethyl loperamide, from blood.
Using the Develosil UHPLC C18 and C30 for Oligonucleotide Analysis
February 01, 2019
Pharmaceutical/Drug Discovery
Here we applied our newly developed UHPLC C18 and C30 columns to oligonucleotide analysis.
Simultaneous Analysis of Ten Water-Soluble Vitamins With a Polymer-Based Reversed-Phase Column– Shodex™ RSpak DE-413L
February 01, 2019
Pharmaceutical/Drug Discovery
In this application, a simple method to simultaneously analyze various water-soluble vitamins was developed.