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A method is illustrated for the simultaneous analysis of nicotine, anabasine, and three of nicotine’s major metabolites (cotinine, nornicotine, and 3-hydroxycotinine) using the Kinetex 2.6 μm EVO C18 column.

Achieve excellent peak shape and resolution between the broad spectrum antibiotic cefaclor and its degredants using the unique selectivity and high efficiency of a Kinetex EVO C18 core-shell.

Achieve excellent peak shape and resolution between the broad spectrum antibiotic cefaclor and its degredants using the unique selectivity and high efficiency of a Kinetex EVO C18 core-shell.

Rapid and comprehensive resolution of a cold medicine API screen that can easily be implemented on HPLC and UHPLC instrumentation using a high performance core-shell Kinetex 2.6 μm EVO C18 column

Rapid and comprehensive resolution of a cold medicine API screen that can easily be implemented on HPLC and UHPLC instrumentation using a high performance core-shell Kinetex 2.6 μm EVO C18 column

A high performance, pH stable core-shell Kinetex EVO C18 column obtains narrow peak shape and extremely useful resolution for a doxylamine formulation impurity profile.

Highly sensitive and reliable screen of Benzodiazepines, Opiates, Synthetic Opioids, Amphetamines, Analgesics and Illicit Drugs

A high performance, pH stable core-shell Kinetex EVO C18 column obtains narrow peak shape and extremely useful resolution for a doxylamine formulation impurity profile.

Enjoy the unique Kinetex EVO C18 selectivity now in 1.7µm and 2.6µm particle sizes for your UHPLC work. This extremely versatile core-shell phase offers the high performance you crave, a unique all-purpose selectivity, and ruggedness you can depend on.

Highly sensitive and reliable screen of Benzodiazepines, Opiates, Synthetic Opioids, Amphetamines, Analgesics and Illicit Drugs

Click the title above to open The Column June 05, 2015 Europe & Asia issue, Volume 11, Number 10, in an interactive PDF format.

Click the title above to open The Column June 05, 2015 North American issue, Volume 11, Number 10, in an interactive PDF format.

Click the title above to open the LCGC Europe June 2015 Recent Developments in HPLC and UHPLC Supplement, Vol 28, No s6, in an interactive PDF format.

Environmental sample analysis by large-volume injection (LVI) in combination with liquid chromatography–tandem mass spectrometry (LC–MS-MS) is described for polar and nonpolar analytes in both aqueous samples and organic extracts.

Unexpected results from calibration standards create confusion in a clinical liquid chromatography (LC) method.

Readers’ questions regarding problems related to internal standard calibration of liquid chromatography methods are addressed.

This instalment provides an overview of modern practices of high-throughput purification to support small-molecule drug discovery. It describes the use of reversed-phase liquid chromatography (LC) and supercritical fluid chromatography (SFC) for purifying diverse samples in a centralized laboratory setting. A case study is used to illustrate the principles and rationales for selecting operating parameters for these applications. This is the first instalment of four articles on “Separation Science in Drug Development” to describe the modern practice of separation science in supporting small-molecule drug discovery and development.

The HPLC Troubleshooting app is designed to help beginner HPLC users with HPLC troubleshooting.

Temperature affects not only retention but also relative retention in gas chromatography (GC) and therefore, when we change temperature, we also change the selectivity of the separation. This is true as we alter the isothermal separation temperature, but also as we change the slope of the temperature program gradient.

Enhanced-fluidity liquids are organic solvents or organic–aqueous solvents mixed with high proportions of liquefied gases, such as carbon dioxide.

Introduction from the Guest Editor.

A liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI–MS–MS) method has been developed to determine multiple preservatives in cosmetics and personal care products.

The capabilities of enhanced-fluidity liquid chromatography for highly polar compounds is described and the value of using the entire continuum of 0–100% carbon dioxide solvent systems is discussed in terms of changes in mobile‑phase properties and applications.

A novel approach to enhancing the selectivity of ionizable compounds using superficially porous particles that are stable in a wider pH range is reported here.

Internal standard methods are used to improve the precision and accuracy of results where volume errors are difficult to predict and control. A systematic approach has been used to compare internal and external standard methods in high performance liquid chromatography (HPLC). The precision was determined at several different injection volumes for HPLC and ultrahigh‑pressure liquid chromatography (UHPLC), with two analyte and internal standard combinations. Precision using three methods of adding the internal standard to the analyte before final dilution was examined. The internal standard method outperformed external standard methods in all instances.