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Extraneous peaks, unrelated to the solute to be characterized, are quite common in gel permeation chromatography/size-exclusion chromatography (GPC/SEC), especially when refractive index (RI) detection is used. This instalment of Tips & Tricks explains why system or ghost peaks appear and how to minimize their appearance.

A surge in the development and commercialization of monoclonal antibody (mAb)-based ther-apeutics has driven the requirement for accurate and reproducible analytical methods for pro-tein characterization. Monoclonal antibodies are popular biologic drug candidates, but are sus-ceptible to a myriad of modifications during manufacture and complex degradation pathways during purification and storage, often leading to distinct charge variants that require character-ization and quantification. Ion‑exchange liquid chromatography (IEX) is a well-accepted and widely used technique to separate various mAb charge variant species for the sake of charac-terization and profiling. With the most recent advances in analytical technologies, IEX can be used to help ensure the selection of stable and efficacious mAb drug candidates, from discov-ery through manufacturing.

Researchers from Postnova have used asymmetrical flow field-flow fractionation coupled to AF4-MALS to successfully characterize a polydisperse cellulose nanocrystal for size and thickness.

Researchers from the PUC-Rio in Rio de Janeiro, Brazil, have found unexpectedly high uranium concentrations in groundwater samples from the mountainous region near Rio de Janeiro City.

Chiral Technologies Inc. has announced the acquisition of Arbor Biosciences.

Researchers from Hacettepe University, Ankara, Turkey, have developed an UHPLC–MS/MS method to determine serotonin in raw and roasted nuts and nut products, including bars, chocolate, and spreads.

Click the title above to open The Column February 2019 North American issue, Volume 15, Number 2, in an interactive PDF format.

Click the title above to open The Column February 2019 Europe & Asia issue, Volume 15, Number 2, in an interactive PDF format.

Two-dimensional gas chromatography (GCxGC) is becoming the technique of choice for analysis of highly complex samples such as petroleum, pharmaceuticals, biological materials, food, flavors, and fragrances. Here, we explain how GCxGC works and provide examples that illustrate its advantages.

Data integrity is paramount when working in a regulated environment. Data process mapping is an excellent way to identify and mitigate data gaps and record vulnerabilities in a chromatographic process. This approach is simple and practical.

The contributions of Milos V. Novotny and Ken Broeckhoven, the winners of the 2019 LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, are presented.

It is often possible to achieve better sensitivity and lower limits of detection and quantitation using standard gas chromatography instrumentation. Paying attention to fundamental variables-like sample solvent, initial oven temperature hold, carrier gas operating mode, and thermal gradients- can lead to much improved method performance.

When can we use completely aqueous eluents with reversed-phase stationary phases, and what happens if we make a mistake?

This instalment of “GC Connections” begins with a brief introduction to GC×GC, follows with examples of how GC×GC opens additional avenues of analysis, and it concludes with information about how to learn more.

Tips to improve signal-to-noise ratio when using standard gas chromatography (GC) equipment.

The third meeting in the “Emerging Separations Technologies” series will take place on Thursday 28 March 2019 at RSC Burlington House, Piccadilly, London.

LCGC Asia Pacific spoke to Yong Liu and Adam Socia from MSD about the cost-saving benefits of implementing green chromatography in the pharmaceutical sector, the importance of analytical method volume intensity (AMVI), and effective practices to reduce solvent consumption and replace harmful solvents, including supercritical fluid chromatography (SFC), fast chromatography, and “cocktail chromatography”.

Click the title above to open The Column January 2019 North American issue, Volume 15, Number 1, in an interactive PDF format.

Click the title above to open The Column January 2019 Europe & Asia issue, Volume 15, Number 1, in an interactive PDF format.

I’ve written several times on the operational principles and variables used in UV detection, but I also get asked questions on the basic theory of UV detection, and this time I’m going to address some of those FAQs to give a little more background theory as well as some operational tips and tricks.

The UK Department of International Trade (DIT) has awarded Markes International with a trade accolade in recognition of its success in exporting scientific instrumentation.

A novel flow-confinement concept designed to remove the performance sacrifice associated with current comprehensive two-dimensional liquid chromatography techniques has been tested by researchers.

The EAS Award for Outstanding Achievements in Separation Science was awarded to Christopher Pohl at EAS on 12 November.

The EAS Award for Outstanding Achievements in the Fields of Analytical Chemistry was presented to Linda B. McGown at EAS on 14 November.

The 9th SCM symposia (SCM-9) will take place from 30 January to 1 February 2019, in Amsterdam, The Netherlands. Focusing on the separation and characterization of natural and synthetic macromolecules, SCM is the place where academia is challenged by industry to help solve real problems using analytical tools.

The EAS Young Investigator Award was presented to Kerri Pratt at the EAS, in Princeton, New Jersey, USA, on 12 November.

You may be one of the many analytical scientists who look with envy at those laboratories who are equipped with sophisticated automated HPLC method development systems. These systems are indeed very nice and can be very efficient in narrowing down choices, however, they aren’t a universal panacea and one can achieve a lot with a simple, paired down approach.