Application Notes: General

Quantifies affinity, stoichiometry of biomolecular assemblies, helping understand the mechanism of interaction and develop therapeutics that target and modulate them.

Quantifies affinity, stoichiometry of biomolecular assemblies, helping understand the mechanism of interaction and develop therapeutics that target and modulate them.

The advantage of UV spectroscopy as an analytical method to detect protein aggregation is that it is non-destructive and uses low sample volumes, minimal sample preparation requirement and easy sample analysis. A quick QC method to indicate the presence of aggregation uses an Agilent Cary 60 UV-Vis spectrophotometer to identify aggregation of mAb solutions resulting from different stress conditions.

This study shows that the organic compounds giving rise to the flavor of beverages can be identified by high-capacity sorptive extraction using PDMS probes, wih analysis by thermal desorption–GC–MS.

Smaller particle size stationary phases have seen increased use in the modern analytical laboratory due to their ability to exhibit greater resolution while enabling the use of smaller column geometries. Shorter column lengths and narrower bores allow for faster run times and lower flow rates, decreasing laboratory costs in terms of solvent consumption and man-hours needed to run the analyses. This application note examines the effects of smaller particle sizes in various column geometries on size-exclusion chromatography (SEC) methods and the method variables affecting the characterization of antibody products.

This work focused on scaling-down a monoclonal antibody SEC method, starting with a typical analytical-size SEC column (YMC-Pack Diol 5um, 300Å, 300x8.0mm) and ending with a small particle, shorter length, narrower-bore column (YMC-pack Diol 2um, 300Å, 150x4.6mm). The purpose of this experiment was to detail the differing performance characteristics exhibited by each column as physical properties were altered, one variable at a time.

In the United States, the increasing acceptance of medical and recreational cannabis at the state level has created a need for accurate, precise and efficient analysis of cannabinoids in marijuana flower, extracts, and formulated products.

The poster describes how different suppressors (MSM and MCS) work and mentions possible applications.

Chromatographic conditions were developed for a fast GC-MS glycol ether analysis on the Rxi®-1301Sil MS column. This cyanobased thin film column provides better resolution and faster run times than the thick film cyanopropylphenyl-type columns commonly used for speciation of the glycol ethers. The glycol ethers are high production volume industrial chemicals that often occur as complex mixtures of isomers. The Rxi®-1301Sil MS column is uniquely matched for the separation of these isomers while still producing narrow and symmetric peaks for the low molecular weight ethylene glycol ethers.

This poster presents an approach that couples a Particle-Into-Liquid-Sampler (PILS) to a dual-channel ion chromatograph (IC) for measurement of aerosol anions and cations and a voltammetric measuring stand (VA) to determine the heavy metals. Feasibility of the PILS-IC-VA online system was demonstrated by collecting aerosol samples in Herisau Switzerland, at defined time intervals; air pollution events were simulated by burning lead- and cadmium-coated sparklers.

The combination of ion chromatography (IC) and inductively coupled plasma mass spectrometry (ICP/MS) provides a rapid, reliable, and sensitive speciation analysis of wastewater-relevant free and complexed gadolinium compounds. IC-ICP/MS proceeds without costly sample preparation and provides important information on the supply, degradation, and fate of the contrast agents in the (waste)water. The method is also highly suitable for determining compounds containing gadolinium in biological matrixes such as urine or blood. Additionally, IC-ICP/MS is a powerful tool for monitoring inorganic and iodine-containing ionic oxidation by-products that form during ozonation of iodinated X-contrast media.

This poster looks at the possibility to modify the existing EPA Method to meet California's rigorous public health goal (PHG) of 0.02 µg/L. After optimizing instrument settings and method parameters, a method detection limit (MDL) of 0.01 µg/L is obtained.

This poster discusses results showing the influence of pH, temperature of the post-column reactor, eluent composition, and iodide concentration on the sensitivity of the triiodide method.

The Combustion IC system presented allows the automated determination of organic halogen and sulfur compounds in all flammable samples. Both combustion digestion, which is automatically controlled with a flame sensor, and the professional Liquid Handling guarantee highest precision and trueness. This poster describes the determination of the halogen and sulfur content in a certified polymer standard, a coal reference material as well as in latex and vinyl gloves.

Method transfer or method scaling across different LC instruments can be affected by both dwell volume and extra-column dispersion. Each characteristic will have a different effect on the chromatographic separation.

With the increase of research with Eudragit, consistent molecular weight measurements have become more prevalent. Shodex has shown a repeatable SEC method using the GF-510 HQ column.

This application note shows how the combination of column chromatography (SEC/GPC) with Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and RI detection can be applied as a powerful tool to identify monomers, dimers, hexamers and higher aggregates of insulin.

This application note shows how the combination of column chromatography (SEC/GPC) with Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and RI detection can be applied as a powerful tool to identify monomers, dimers, hexamers and higher aggregates of insulin.

Traditional extraction typically involve intensive manual LLE shakes at both pH 2 and 12 prone heavy emulsions and low recoveries or CLLE for traditional base, neutral, and acid for semivolatiles extractions can take up to 24hrs. By incorporating automated SPE with multi-bed sorbents, base, neutral, and acid for semivolatiles samples can be extracted with a single pass procedure without emulsions, centrifuging, and hours of manual labor. The FMS TurboTrace ABN SPE system is designed to handle multiple cartridges and provides a fully automated solution for the semivolatile extraction process.