Application Notes: General

This poster presents an approach that couples a Particle-Into-Liquid-Sampler (PILS) to a dual-channel ion chromatograph (IC) for measurement of aerosol anions and cations and a voltammetric measuring stand (VA) to determine the heavy metals. Feasibility of the PILS-IC-VA online system was demonstrated by collecting aerosol samples in Herisau Switzerland, at defined time intervals; air pollution events were simulated by burning lead- and cadmium-coated sparklers.

The combination of ion chromatography (IC) and inductively coupled plasma mass spectrometry (ICP/MS) provides a rapid, reliable, and sensitive speciation analysis of wastewater-relevant free and complexed gadolinium compounds. IC-ICP/MS proceeds without costly sample preparation and provides important information on the supply, degradation, and fate of the contrast agents in the (waste)water. The method is also highly suitable for determining compounds containing gadolinium in biological matrixes such as urine or blood. Additionally, IC-ICP/MS is a powerful tool for monitoring inorganic and iodine-containing ionic oxidation by-products that form during ozonation of iodinated X-contrast media.

This poster looks at the possibility to modify the existing EPA Method to meet California's rigorous public health goal (PHG) of 0.02 µg/L. After optimizing instrument settings and method parameters, a method detection limit (MDL) of 0.01 µg/L is obtained.

This poster discusses results showing the influence of pH, temperature of the post-column reactor, eluent composition, and iodide concentration on the sensitivity of the triiodide method.

The Combustion IC system presented allows the automated determination of organic halogen and sulfur compounds in all flammable samples. Both combustion digestion, which is automatically controlled with a flame sensor, and the professional Liquid Handling guarantee highest precision and trueness. This poster describes the determination of the halogen and sulfur content in a certified polymer standard, a coal reference material as well as in latex and vinyl gloves.

Method transfer or method scaling across different LC instruments can be affected by both dwell volume and extra-column dispersion. Each characteristic will have a different effect on the chromatographic separation.

With the increase of research with Eudragit, consistent molecular weight measurements have become more prevalent. Shodex has shown a repeatable SEC method using the GF-510 HQ column.

This application note shows how the combination of column chromatography (SEC/GPC) with Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and RI detection can be applied as a powerful tool to identify monomers, dimers, hexamers and higher aggregates of insulin.

This application note shows how the combination of column chromatography (SEC/GPC) with Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and RI detection can be applied as a powerful tool to identify monomers, dimers, hexamers and higher aggregates of insulin.

Traditional extraction typically involve intensive manual LLE shakes at both pH 2 and 12 prone heavy emulsions and low recoveries or CLLE for traditional base, neutral, and acid for semivolatiles extractions can take up to 24hrs. By incorporating automated SPE with multi-bed sorbents, base, neutral, and acid for semivolatiles samples can be extracted with a single pass procedure without emulsions, centrifuging, and hours of manual labor. The FMS TurboTrace ABN SPE system is designed to handle multiple cartridges and provides a fully automated solution for the semivolatile extraction process.

This application note demonstrates that Markes International’s automated thermal desorption systems offer excellent results for monitoring volatile organic compounds (VOCs) in stationary source emissions in accordance with the updated version of the European standard method CEN/TS 13649 released in 2014. The value of repeat analysis for method development and result verification is also demonstrated.

What is thermal desorption and how can it extend the capability of GC? In this podcast, you’ll hear the answers to these questions directly from Elizabeth Woolfenden, a leading expert on this sample introduction technique. As a co-founder of Markes International, widely acknowledged as the world leaders in TD, Elizabeth has been right at the heart of the development of thermal desorption, and will draw on her experience to highlight the scope of the technique, along with key technical innovations and emerging applications. So whether you’re new to TD and want a quick introduction, or an experienced analyst interested in hearing a personal perspective, don’t miss this unique opportunity to get the lowdown on thermal desorption from a major player in the area!

This application note describes a UPLC-MSE analysis of monoclonal antibody (mAb) disulfide bond. This study demonstrates that UNIFI-a compliance-ready software-is designed to support biopharmaceutical laboratories in performing fast peptide mapping and monitoring of mAb disulfide linkages.

This whitepaper provides a summary of the impact of PIC/S, and compliance with the PIC/S document PE 009-12 “Guide to Good Manufacturing Practice for Medicinal Products Annexes“ Annex 11 Computerised Systems. It includes a high-level foundation to understand how pharmaceutical manufacturing companies are being regulated, what PIC/S means for common requirements across different countries, and how these requirements relate to expectations for data from computerised systems/electronic records.

The quick capture and accurate titer analysis over a wide concentration range of monoclonal antibody is demonstrated using a TSKgel® Protein A-5PW, 20 μm analytical column.

Reflectron lenses are used in time-of-flight (TOF) mass spectrometers to create an electrostatic field to alter ion flow, providing for a longer flight path and therefore greater resolution.

The development of an automated online SPE–LC–MS-MS method for the analysis of 25-OH-vitamin D2, 25-OH-vitamin D3, and 3-Epi-25-OH-vitamin D3 from human serum.

Enhancing the extraction of vitamin A and E in serum using simplified liquid extraction (SLE) significantly improves the LC–MS-MS detection of target compounds without an overly complex method or sacrificing robustness.

The development of an automated online SPE–LC–MS-MS method for the analysis of 25-OH-vitamin D2, 25-OH-vitamin D3, and 3-Epi-25-OH-vitamin D3 from human serum.

A full ESI+/ESI- 52 drug panel was performed in 7 min and 30 s, including re-equilibration (44 in ESI+ and 8 in ESI-). This rapid analysis showed great resolution and no loss in sensitivity compared to slower methods.

A full ESI+/ESI- 52 drug panel was performed in 7 min and 30 s, including re-equilibration (44 in ESI+ and 8 in ESI-). This rapid analysis showed great resolution and no loss in sensitivity compared to slower methods.

Enhancing the extraction of vitamin A and E in serum using simplified liquid extraction (SLE) significantly improves the LC–MS-MS detection of target compounds without an overly complex method or sacrificing robustness.

A simple automated extraction method using a Tecan Freedom EVO® 100 liquid handler and Phenomenex’s Novum simplified liquid extraction (SLE) 96-well plate. Analysis of isomeric cortisone and prednisolone by UHPLC by Kinetex 2.6 μm, 50 x 3 mm core-shell Biphenyl column was used to successfully separate these two compounds and their isomers.

A simple automated extraction method using a Tecan Freedom EVO® 100 liquid handler and Phenomenex’s Novum simplified liquid extraction (SLE) 96-well plate. Analysis of isomeric cortisone and prednisolone by UHPLC by Kinetex 2.6 μm, 50 x 3 mm core-shell Biphenyl column was used to successfully separate these two compounds and their isomers.

Using a single LC–MS-MS application, 12 steroids were separated. However, three separate panels are also run as an alternative method.

This application note describes the GC¬¬–MS analysis of 23 drugs of abuse from various compound classes such as amphetamines, benzodiazepines, cannabinoids, and other illicit drugs.