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The most commonly applied detector in gel permeation chromatography/size-exclusion chromatography (GPC/SEC) is the differential refractive index detector, RI. How UV–vis detection, if applicable, adds true value to GPC/SEC applications is discussed in this instalment of Tips & Tricks.

Splitless injections are sometimes necessary for trace analyses, where the analyst hopes to recover 100% of the analytes that are injected. Unfortunately, splitless injections can be challenging and using an imperfect method can lead to loss of analytes and poor peak shapes. The choice of inlet liner can have an impact on the data and one must consider the effects of geometry, packing, deactivation, and volume on introduction of analytes into the system. Other important inlet parameters to consider include inlet temperature, splitless hold time, and initial oven temperature.

This article highlights the use of a fast and simple high performance liquid chromatography–ultraviolet (HPLC–UV) assay for separation and quantification of 11 important cannabinoids, including CBD in hemp oil.

Incognito asks if chromatographers are behind the curve when it comes to automation.

The key differences between a classical balance and currently available automated systems from the good weighing practice (GWP) perspective and within the scope of ISO9001:2015 quality standard are discussed. The systems under review are: (i) manual analytical balances; (ii) semi‑automatic systems; (iii) fully automatic systems; and (iv) integrated systems that use on-line coupling of the analytical balance with the instrument used for analytical measurements. The parameters defined in GWP guidelines, such as accuracy, uncertainty, minimum weight, and risks (including out‑of‑specification results), will be evaluated for all of these systems.

Researchers from Merck and Agilent Technologies have developed a simple and fast generic gas chromatography–flame ionization detection (GC–FID) method for the quantitation of volatile amines in pharmaceutical drugs and synthetic intermediates.

Agilent Technologies has announced that it has entered into a definitive agreement to acquire the privately held ProZyme, Inc., a provider of glycan analysis reagents, kits, and standards.

Hamilton Storage GmbH has announced the total relocation of its operations to a high-technology industrial park in Domat/Ems, Switzerland.

The Chromatographic Society has announced the “Grass Roots 3” Educational Event is to be held at the Waterhead Hotel, Ambleside, Cumbria, UK, Friday 5–Monday 8 October 2018.

Click the title above to open The Column July 2018 Europe & Asia issue, Volume 14, Number 7, in an interactive PDF format.

It is often possible to achieve better sensitivity and lower limits of detection and quantitation using standard gas chromatography (GC) equipment-here I’m referring to a standard split/splitless injection port and a Flame Ionization Detector (FID). Paying attention to some of the fundamental variables as well as some of the more esoteric considerations can lead to much improved method performance.

If given the need to determine drug A or its metabolite in blood, 99% of the time I would choose to start with liquid chromatography–mass spectrometry (LC–MS).

Click the title above to open the LCGC Europe June 2018 regular issue in an interactive PDF format.

Current Trends in Mass Spectrometry, July 2018

The use of superficially porous particles (SPPs) for modern high performance liquid chromatography (HPLC) is now very common. Initially, SPPs rose as an alternative to sub-2-µm fully porous particles (FPPs). In recent years, many column manufacturers have developed 2-µm and smaller SPP-based products. This article investigates the practical utility of these smaller SPP designs.

Many manufacturers now offer columns using superficially porous particles that are 2-µm and smaller. When should you use them?

While capillary gas chromatography has been undergoing a renaissance, with new columns, detectors, data systems, and multidimensional separations, the classical inlets have remained the same: We are still injecting liquid samples with syringes into split and splitless inlets, as we have for nearly 50 years. Split and splitless injections present several well-known and some not-so-well known challenges, mostly arising from heating of the inlet, that make sample injection and inlets a major hurdle for gas chromatographers. These challenges and some ideas for mitigating them are discussed and a case is made for renewed exploration of the cool inlets and injection techniques: cool on-column and programmed temperature vaporization.

The 32nd International Symposium on Chromatography (ISC 2018) will be held on 23–27 September 2018 in Cannes-Mandelieu, France.

We discuss the challenges of split and splitless injections, ideas for mitigating them, and the case for renewed exploration of cool inlets and injection techniques.

This is the first of three articles looking at the impact of the new United States Pharmacopeia (USP) chapter on Analytical Instrument Qualification (AIQ) on data integrity in a regulated chromatography labora-tory. In part 1, user specifications for chromatography systems and the relationship between users and sup-pliers will be discussed.

The Chromatographic Society and the Royal Society of Chemistry Analytical Division North East Region has organized a meeting to be held on 22 November 2018 in the Stadium of Light, Sunderland, UK.

To extend the life of your GC columns, follow these best practices.

You need to ensure that your calculations of reportable results don’t get you into data integrity trouble. Here’s how.

This study of PAHs makes it easy to compare this microextraction procedure to conventional approaches and to other procedures using magnetic composites.

What type of mass spectrometry (MS) instrumentation provides the best specificity during trace quantitative analysis from complex mixtures?

We review different approaches for measuring solute retention.