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Dynamic light scattering (DLS), widely recognized for its ability to detect aggregation in purified protein solutions, is actually far more versatile and may be applied to the evaluation of stability and viscosity of therapeutic proteins and other biopharmaceuticals. In a fully automated, microwell plate reader format, DLS constitutes a high-throughput biophysical screening technique for early-stage assessment of candidate developability and optimal formulation conditions.

Click the title above to open The Column June 20, 2017 Europe & Asia issue, Volume 13, Number 9, in an interactive PDF format.

Click the title above to open The Column June 20, 2017 North American issue, Volume 13, Number 9, in an interactive PDF format.

When you want to adjust a United States Pharmacopeia (USP) method for a different size column or to meet system suitability criteria that fail, how much of a change can you make without revalidating the method?

The quantitative extraction and subsequent purification of trace contaminants from (semi-)solid environmental and food matrices of regular size (that is, a few grams) is still recognized as a challenging task, typically accomplished through relatively complex off-line multistep treatment procedures. When these conventional sample preparation procedures are applied to the treatment of size-limited samples (of less than 1 g), the difficulties increase. This review discusses the different analytical strategies that can be adopted to overcome (or at least reduce) these difficulties when chromatographic techniques are involved for final instrumental determination.

hermo Fisher Scientific has announced its intention to buy Patheon NV.

A novel unit that integrates sampling and analysis for the determination of pest insect sexual pheromones in environmental air using fabric phase sorptive extraction (FPSE) and headspace gas chromatography coupled to mass spectrometry (HSGC–MS) has been developed at the University of Córdoba in Spain.

Peak Scientific has expanded its operations in Latin American (LATAM), moving to a new, larger premise with the aim of developing its support capabilities in the growing LATAM market.

Molecular mass is one of the central parameters required for product registration. Compared to low molar mass substances, the molar mass determination of macromolecular products is more difficult because the product is a mixture of chains with different lengths and, therefore, molar masses. Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) is the standard technique to separate macromolecules by size and to measure the complete molar mass distribution as well as the molar mass averages. This technique therefore provides crucial information for product registration, including REACH.

A preview of HPLC 2017, which will be held from 18 to 22 June 2017 at the Prague Congress Centre, Prague, Czech Republic.

Analysis of residual antimicrobials in animal products is crucially important to ensure food safety. This article presents a simple, fast, and highly sensitive high performance liquid chromatography (HPLC) assay of regulated antimicrobials, as well as sample preparation and purification methods. Twenty-four different analytes of interest were investigated in beef, pork, and chicken meat samples.

Successful therapeutic intervention often requires chiral medicines because of the intrinsic chirality of protein drug targets, which consist of L-amino acids. Potency, efficacy, and safety can be highly dependent on the precise stereochemical geometry of the molecules. Determining the biological profile of individual enantiomers in the early stages of drug discovery is important for successful optimization towards clinical candidates. Here we demonstrate the benefits of supercritical fluid chromatography (SFC) with three chiral stationary phases exemplified by high frequency resolution of 41 out of 50 chiral derivatives of eight commonly used drug discovery scaffolds including 1,3-thiazoles, 1,3-benzothiazoles, pyranoquinolones, indoles, and leucolines.

A preview of the HPTLC 2017 symposium, which will take place this July.

Click the title above to open The Column June 06, 2017 Europe & Asia issue, Volume 13, Number 8, in an interactive PDF format.

Click the title above to open The Column June 06, 2017 North American issue, Volume 13, Number 8, in an interactive PDF format.

I’m often asked “what reproducibility should I expect to get from my [insert instrument manufacturer and model]?” So, most folks are referring to the repeatability aspects of precision, as in: “what relative standard deviation (usually expressed at %RSD) for peak area or quantitative result should I be able to achieve from repeat injections from a single vial of sample?”

Click the title above to open the LCGC North America June 2017 Application Notebook, Vol 35 No s6, in an interactive PDF format.

Precise and accurate quantitative analysis based on chromatographic measurements has historically relied very heavily on careful peak integration. Seasoned analysts know that while automated algorithms exist in modern chromatography software, it is a best practice to manually check that the integration points-the points at the beginning and end of a peak, between which the peak will be integrated to obtain a peak area-are appropriately specified.

This information is supplementary to the article “Utility of the Summation Chromatographic Peak Integration Function to Avoid Manual Reintegrations in the Analysis of Targeted Analytes” that was published in the June 2017 LCGC North America issue (1). 

An excerpt from LCGC’s e-learning tutorial on high performance liquid chromatography (HPLC) methods at CHROMacademy.com

When you want to adjust a United States Pharmacopeia (USP) method for a different size column or to meet system suitability criteria that fail, how much of a change can you make without revalidating the method?

This yearly report on new products introduced at Pittcon 2017 (or in the preceding year) covers sample preparation instrumentation, supplies, and accessories.

In reversed-phase liquid chromatography (LC), C18 alkyl-based stationary phases have been the favourite of method developers. Phenyl stationary phases are an alternative that are thought to benefit from additional π-π mechanisms. Recently, there has been a growing interest in the use of phases based on the biphenyl moiety. This instalment of “Column Watch” looks at the retention mechanisms of biphenyl phases and contrasts them with those of more-common alkyl phases.

The 24th International Symposium on Electro- and Liquid Phase- Separation Techniques (ITP 2017) will be held in Sopot, Poland, at the Sheraton Hotel, 10–13 September 2017.

In biphenyl phases, the surface chemistry often makes it possible to separate compounds not well resolved by C18 or phenyl phases.

How much of a change can you make to a USP method without revalidating the method?