October 9th 2024
As part of our ISC 2024 coverage, we recently interviewed Anneli Kruve of Stockholm University about her scientific background and her presentation about eludicating toxic chemicals' structures using LC/HRMS.
Known and Novel Proteins in Pseudomonas stutzeri Detected by Bottom-Up Proteomics
August 18th 2023The combination of the “bottom-up” approach with digest-free, direct-sequencing proteomics, along with the addition of a proteogenomics pipeline that identified missing data, yielded the determination of almost 3000 proteins.
Review of Packed Sorbent Solid-Phase Extraction and Microextraction Methods From Manual to Automated
August 15th 2023Studies published over the last five years have focused on fully or semi-automated approaches and their applications in extraction of both organic and inorganic pollutants, while modern robotic tools and 3D printing are starting to make impacts.
Enantioselectivity in LC Evaluated by Variations in Modifiers and Modifier Concentration
August 11th 2023Since similar chiral recognition mechanisms were observed for benzoin (B) and methyl mandelate (MM), a three-site enantioselectivity model was proposed to interpret the data being sought by researchers.
Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease Digest
August 1st 2023Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.
A Modern Mineral Oil Hydrocarbon Analysis
August 1st 2023Giorgia Purcaro of the University of Liège discusses the advantages offered by the liquid chromatography–comprehensive multidimensional gas chromatography time-of-flight mass spectrometer/flame ionization detector (LC–GCxGC–TOF-MS/FID) method in the analysis of mineral oil.
Polysorbate Analysis in Biopharmaceutical Applications—A Snapshot of the Analytical Toolbox
An overview of different approaches for the qualitative and quantitative analysis of polysorbates.
CIL-UHPLC–HRMS Profiles Carbonyl Compounds in Baijiu Daqu Samples
July 27th 2023Sauce-flavor or sauce-aroma baijiu, one variant of a popular Chinese liquor, is treated with a fermentation starter called daqu, giving the concoction a unique taste profile, and scientists want more information about how it can inform the manufacturing of higher-quality baijiu.