Liquid Chromatography (LC/HPLC)

This month's "LC Troubleshooting" discussion will center on the recommendations of the CDER document, especially in terms of what it means from a practical method performance standpoint.

Guest columnist Dick Henry traces the history of HPLC back more than 40 years, starting with his days at DuPont.

Using a fixed length-variant of the kinetic plot method, it is illustrated how an analysis that is performed near the optimal flow-rate of a given commercial column can, in many cases, be performed between 50–200% faster by switching to a longer column and operating it at a higher pressure - at least, if the available instrument pressure admits so. The present article aims to show that short columns are not always the best choice to get the fastest separation.

Dionex has entered into an agreement with Caliper Life Sciences to purchase Caliper's AutoTrace line of products...

Italian researchers in Rome (Universita Cattolica and Consiglio Nazionale delle Ricerche) and Cagliari (Universita di Cagliari) have used reversed-phase high performance liquid chromatography with electrospray ionization mass spectrometry to analyze naturally occurring salivary peptides from subjects with autism and compare the profiles with those obtained from age-matched control subjects.

At the beginning of the year, it is good to look back in time- are there things that we can learn from the past? After a series of troubleshooting and method development seminars in Istanbul, Turkey, and Amman, Jordan, John Dolan looks back.

This second part of the series describes the data loss inherent in most early method development experiments due to coelution, peak exchange, and the general difficulty of accurately identifying peaks across the experiment trial chromatograms.

Guest authors show how mixed modes can be used successfully in the optimization of protein purification, and discuss how various experimental parameters can be used to regulate the binding of proteins to mixed-mode sorbents.

Dr. Philip J. Wyatt, the CEO and founder of Wyatt Technology Corporation (Santa Barbara, California) has been awarded the 2009 Prize for Industrial Application of Physics by the American Physical Society (APS).

Phenomenex Inc., (Torrance, California), will present a workshop, "A Critical Analysis of Fast LC Technologies," at the 2009 Pittsburgh Conference in Chicago.

Pittcon announced the recipients of 11 prestigious awards recognizing the significant contributions of leaders in analytical chemistry and applied spectroscopy.

Goal: Positively identify trace amounts of cannabinoids in a complex food matrix quickly, with minimal sample preparation and no chemical derivatization.

A highly sensitive analytical method for the analysis of tamsulosin in human plasma has been developed for use in bioanalytical studies. The solid-phase extraction (SPE) and UPLC–MS–MS methodologies are described, as well as performance against validation parameters.

An ultrafast gradient LC separation method was developed to separate a 5-component drug mixture in 30 seconds, with peak widths of 1 second. maXis mass accuracy at sub-ppm levels and true isotopic pattern of the spectra from the peaks lead to a confident elemental formula assignment for each drug compound with the SmartFormula algorithm.

Cytosine (chemical name 4-amino-2-hydroxypyrimidine) is a pyrimidine derivative with a hetereocyclic aromatic ring and two substituents (amine and keto groups) attached and is a polar compound of significant biological and pharmaceutical interest. In response to the intended use of bulk cytosine as a raw material in pharmaceutical manufacturing, a method for the determination of the purity of cytosine was developed.

Scientist Cornelia Kasper and colleagues from the Gottfried Wilhelm Leibniz University of Hanover (Hanover, Germany) thought that a liquid chromatography method with short monolithic columns would be a quicker alternative to measure proteins in culture while also having had the potential to separate different proteins.

Thermo Fisher Scientific Inc., (San Jose, CA) has launched a program focused on assisting government and commercial food testing laboratories in the development and implementation of methods for the analysis of melamine in food products imported from China.

This month's "LC Troubleshooting" installment will take a look at the design of these durable pumps and also examine some of the potential weaknesses and how to overcome them.

This installation of "Validation Viewpoint" describes how statistically rigorous quality-by-design (QbD) principles can be put into practice to accelerate each phase of liquid chromatography (LC) instrument method development.

The 47th Eastern Analytical Symposium and Exposition presented its award for Outstanding Achievements in Separation Science to Professor Pat J. Sandra, of Ghent University, Belgium, on November 18, 2008.

All the buzz lately about LC columns packed with particles smaller than 3 mm often comes with warnings about extracolumn effects. This month's installment will take a look at what these effects are and more.

Celebrations for JASCO's 50th anniversary in the analytical instrumentation market will conclude with Jasco Europe, a subsidiary of Jasco Corporation, moving into its new facilities in Cremella, Italy in November 2008.

Varian Inc. (Walnut Creek, CA) and Flora Research Laboratories (Grants Pass, OR) have formed an alliance to advance the understanding and implementation of LC-MS solutions for complex dietary supplement analysis.

A research group from the University of Lisbon (Lisbon, Portugal) studied the use of stir-bar sorptive extraction (SBSE) with polyurethane and polydimethylsiloxane polymeric phases and high performance liquid chromatography with diode-array detection for the analysis of six acidic pharmaceutical compounds in environmental water matrices such as river, sea, and wastewater samples.

The David H. Murdock Research Institute (Kannapolis, NC) announced that Waters (Milford, MA) will be the primary supplier of analytical science solutions, including mass spectrometers, liquid chromatographs, and associated scientific data management software.