The Application Notebook-07-02-2010

The fully automated combustion ion chromatography (CIC) system presented here combines a highly efficient combustion system with the separation power of ion chromatography (IC). CIC allows for the simultaneous, speciated trace analysis of halide (F, Cl, Br and I) and sulphur compounds (as sulphate) from sub-ppm to percent levels in any nonaqueous sample matrix.

Analysis of biological samples frequently involves the identification of peptides from low amounts of complex samples. Confident identification of these peptides requires rapid generation of high quality, high sensitivity MS and MS–MS data. The maXis TOF mass spectrometer incorporates novel technical innovations, which together produce an unprecedented level of data quality, resulting in a significant increase in the number of peptides identified from challenging samples.

Phenol formaldehyde resins are formed by a step-growth polymerization. GPC/SEC with RI and UV detection is often used to characterize resins and to quantify the amount of different oligomeric species.

Ingestion of DEG-adulterated glycerin excipients in pharmaceuticals and personal care products, such as toothpaste, mouth wash and medicinal syrups has caused systemic alcohol intoxication, acidosis and subsequent multiorgan failure that have led to hundreds of fatalities of children and adults.

Grapefruit juice was analysed by gradient UHPLC for naringin - a major flavanoid in grapefruit juice that gives it its bitter taste. A ZORBAX Rapid resolution HD (RRHD) StableBond-C18 2.1 ? 150 mm, 1.8 ?m column was able to resolve over 60 peaks, including naringin in 7 minutes, and was re-equilibrated in just three minutes by operating at a high flow rate (0.8 mL/min). The high pressure generated (965 bar) is well within the 1200 bar operating range of the RRHD column and 1290 Infinity LC.

The analysis of tea tree oil requires a severe quality control due to its potential to induce and elicit skin allergies. Tea tree oil is an essential oil and consists of a large number of constituents. Heart-cut Multidimensional GC–MS is very well suited to analysis of samples of such high complexity.

Pesticides are widely used in agriculture to protect crops and to improve efficiency of production. Consequently, governments, food producers and food retailers have a duty to ensure that any residues occurring in foods for human consumption are at or below statutory maximum residue levels (MRLs). Regulation EC 396/2005 adopted in the European Union sets MRLs for more than 500 different pesticides in over 300 different food commodities.

The release of certain chemicals from building products is of increasing concern due to their potentially detrimental effect on human health and the environment; therefore, new regulations requiring the production of low emission materials are currently being implemented. Novel sampling technology has been developed to help building product manufacturers obtain informative emissions data to develop compliant products and maintain product quality on a routine basis.

This application note deals with the development of a fast method for the determination of 13 aldehyde and ketone derivates with the Agilent 1290 Infinity LC-system. The method, which used acetone as organic co-solvent, separates the analytes within 3.5 minutes.

In pain management analysis, urine is often used as it is a copiously available bodily fluid that can be collected easily and used for analysis.

The costs associated with pharmaceutical testing are considerable, and many lab managers are seeking ways to reduce costs by reducing solvent usage and improving productivity while still using the LC instruments in their lab.

Danshen (Salvia miltrorrhiza) is widely used as a traditional Chinese medicine (TCM) and remedies containing Danshen are traditionally used to treat cardiac and vascular disorders. Danshen dropping pills are a compound preparation consisting of danshen, notoginseng, borneol and other components.

Over-expression of recombinant proteins is commonly used for the production of protein reagents in industry and academia. Problems often occur relating to the stress put on the cells to deal with this huge increase in synthesis.

Transferring LC methods between instruments and/or laboratory sites is a common practice that allows analytical scientists to maximize equipment usage.

For many years, the use of guard columns has been advocated by column manufacturers and other experts to protect and extend column lifetime and performance of analytical columns from potential damage caused by the presence of chemical contaminants and microparticulates in the sample and mobile phase.

Heating is a common and essential requirement in most biopharmaceutical production methods. Chitosan is an amino polysaccharide obtained by alkaline deacetylation of naturally abundant chitin.

Bisphenol A (BPA) is widely used in high production volume manufacturing of polycarbonate plastics (for example, food containers and plastic bottles) and epoxy resins.

Introduction

This paper describes the use of the AB SCIEX TripleTOF 5600 LC–MS–MS system for the screening and identification of unexpected contaminants in lake water, demonstrating the value of high-resolution, accurate-mass data and retrospective data processing for the identification of nontargeted compounds.

Quantification of substances, such as drug impurities or library compounds when pure standards are not available is difficult yet often necessary.

The new 1 mm i.d. ProSwift strong cation-exchange (SCX) column is a polymethacrylate-based monolithic column with sulfonate functionality.

Collision-induced dissociation (CID) and electron transfer dissociation (ETD) are complementary mass spectrometric fragmentation techniques. We have used CID and ETD in different approaches to analyse tyrosine phosphorylation using a Thermo Scientific LTQ Orbitrap XL equipped with ETD.

Methacholine chloride is a synthetic analogue of the neurotransmitter acetylcholine used to assess bronchial asthma of subjects in respiratory function labs and epidemiological field studies.

P-hydroxybenzoic acid esters, also called parabens, are widely used as antimicrobial preservatives in cosmetic products.

Use of UV filters in cosmetic formulations is regulated by international authorities. The concentration of these compounds in cosmetic products is controlled constantly. The following methods describe a simple, fast and ultra fast LC method for determination of UV absorbers. HALO C18* was used as column.

Carbonyl compounds, in particular aldehydes, are reactive volatile substances. They are of concern to the public, because they are emitted as air pollutants by a large range of industrial processes and other combustion sources.

Conclusively determine the make and manufacturer of gunpowders by characterizing their distinguishing ingredients.