The use of superficially porous particles (SPPs) for modern high performance liquid chromatography (HPLC) is now very common. Initially, SPPs rose as an alternative to sub-2-µm fully porous particles (FPPs). In recent years, many column manufacturers have developed 2-µm and smaller SPP-based products. This article investigates the practical utility of these smaller SPP designs.
Several new materials and columns have been introduced in recent years for reversed-phase separations of proteins. How do I know which one to choose, and which separation conditions will be best for my protein separation?
LCGC (LCGC North America, LCGC Europe, and LCGC Asia Pacific) seeks to uphold the highest standards of editorial ethics.
The Chinese American Chromatography Association (CACA) invites nominations for the 2019 CACA Young Investigator Award.
The Chinese American Chromatography Association (CACA) invites nominations for two awards.
Incognito looks to a paradigm shift.
Researchers from ESPCI Paris and the Institut de Recherche Criminelle de la Gendarmerie Nationale have developed and optimized a comprehensive two-dimensional GC–MS method for the forensic profiling of human hand odour.
Agilent Technologies and the University of Duisburg-Essen will collaborate to combine the company’s analytical technologies with the university’s researchers.
Sciex has donated $17,500 to the World Cancer Research Fund (WCRF) to help fund urgently‑needed research investigating the relationships between food, nutrition, and physical activity with cancer prognosis and outcome in cancer survivors.
Researchers from the College of the Holy Cross have developed an ultrafast gas chromatography (UFGC) and chemometric method for the analysis of biodiesel blends.
Click the title above to open The Column August 2018 North American issue, Volume 14, Number 8, in an interactive PDF format.
Click the title above to open The Column August 2018 Europe & Asia issue, Volume 14, Number 8, in an interactive PDF format.
Chromatographic methods often require that the analyte response is calibrated (and validated) over a wide concentration range when the analyte concentration in the sample is either unknown or is expected to vary widely. Bioanalysis, environmental, and clinical applications are just a few examples of where this may be the case.
The 2018 Uwe D. Neue Award in Separation Science was presented to Christopher Pohl of Thermo Fisher Scientific (Sunnyvale, California) during the opening plenary session on Sunday, July 29, at HPLC 2018 in Washington, D.C.
For several years, our group has been working on a concept that we have termed multipath liquid chromatography (LC). The main idea is to target multiple classes of compounds following a single injection of a sample, the components of which are segregated on-line and directed to separate appropriate paths for simultaneous separation; the streams are then recombined for detection. I believe that this approach would be powerful for biomarker quantitation, where it would be more informative to track both metabolite and protein biomarkers to better define a disease state, or in the case of antibody–drug conjugate (ADC) development, where the metabolism of the ADC might involve understanding both the levels of the released drug and the remaining protein.
Peter Nemes of the University of Maryland, College Park (Maryland), was awarded the Georges Guiochon Faculty Fellowship at HPLC 2018 during the opening plenary session on Sunday, July 29, in Washington, D.C.
Click the title above to open the LCGC Europe July 2018 regular issue in an interactive PDF format.
Click the title above to open the LCGC North America August 2018 regular issue in an interactive PDF format.
Blanks are important in the pursuits of quality control and robust quantitative analytical methods, but many analysts don’t fully understand their value, or how to use them correctly.
We examine instances where contaminants are problematic in LC–MS, as well as successful methods to decrease their influence.
We look at a process that can overcome the shortcomings associated with liquid–liquid extraction.
Several new materials and columns have been introduced in recent years for reversed-phase separations of proteins. How do I know which one to choose, and which separation conditions will be best for my protein separation?
This instalment describes ten corollaries in high performance liquid chromatography (HPLC) and pharmaceutical analysis that most practitioners are likely aware of but may overlook the rationales behind them.
Too often, analysts follow prescribed methods, including the processing of “blanks”, without fully understanding the rationale behind the various steps. This month we’ll look at the types of blanks used in an analytical procedure and why they are used. We will focus on those defined by U.S. regulatory agencies.
